Studies on the metabolism of pyruvic acid in normal and vitamin B1-deficient states

Lu, G. D.; Platt, B. S.

doi:10.1042/bj0331538

Cited by 27 publications

(15 citation statements)

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“…It has been shown by several investigators (16)(17)(18) that in thiamine deficiency pyruvic acid gets accumulated in various tissues and body fluids, thus causing an enormous increase in the pyruvic acid level. Thiamine pyrophosphate helps in the oxidative decarboxylation of pyruvic acid to acetyl CoA which on oxidation via Krebs tricarboxylic acid cycle produces a large amount of ATP.…”

Section: Methodsmentioning

confidence: 99%

Influence of Thiamine on Protein Biosynthesis

Chakrabarti

Pandit

1969

J. Vitaminol

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The incorporation experiments with labeled serine in the crude microsomal protein fraction of normal and thiamine deficient rat liver were carried out. The liver microsomal fraction of animals kept on the thiamine deficient diet showed less incorporation of lebeled serine than the normal animals.The injection of thiamine in the thiamine deficient animals two hours before killing increased to an appreciable extent the incor poration of labeled serine in microsomal protein, indicating a possible role of thiamine in protein biosynthesis. Mulgaonkerr and Sreenivasan (6) observed that in a deficiency of vitamin B12 and folic acid in rats there is a significant reduction in total serum proteins.Raskin and Kirzhner (7) reported that patients receiving vitamin Bs and B12 showed more rapid return to normal in serum protein than the patients not receiving vitamin B6 and B12. These studies indicate clearly that besides vitamin B12 there are other vitamins of B groups which may influence protein synthesis.Since much work has not been carried out on the effect of thiamine on microsomal incorporation of labeled amino acid, it has been thought worth-while to investigate whether thiamine has any in fluence on the microsomal biosynthesis of protein. EXPERIMENTALSixty male albino rats weighing between 40 to 50g were selected for this experiment.The animals were divided into two groups o£ thirty each. The animals of group I were fed a normal diet and the animals of group II were given thiamine deficient diet. The composition of the diet for group I and group II is shown in Table 1. The pair-feeding techniques were followed in feeding the animals. The animals were weighed weekly. The normal and thiamine deficient animals were killed periodically by decapitation. The thiamine content of different tissues was estimated by the method of Harris and Wong (8). The blood pyruvic acid was estimated according to the method of Klein (9). The results are shown in Tables 2 and 3.

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Section: Methodsmentioning

confidence: 99%

Influence of Thiamine on Protein Biosynthesis

Chakrabarti

Pandit

1969

J. Vitaminol

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“…Although the probable cause was a temporary disorganization of metabolism following birth, other possibilities have to be considered. In rabbits (Lu, 1939) and in man (Johnson and Edwards, 1937) the blood pyruvic acid is raised after exercise. It is likely that during labour the blood pyruvic acid in the mother will be raised, and that this increase would be shown in the blood of the newbom.…”

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confidence: 99%

“…As the pyruvic acid estimation following transfision was usually performed after an interval of two to three days, adequate time was allowed for stabilization to take place. In normal animals an excess of pyruvate can be removed from the blood in half an hour (Lu, 1939). It seems, therefore, probable that the trnsfud blood, Pyruvk acid in m& per 100 mL by removing or mitigating one or more of the causes contributing to the raised level in the patient's blood, was able to produce a temporary fall in the patient's blood level in the not too severe cases (B.…”

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“…It is suggsted that, where in any case the resting blood pyruvic acid is raised, the response of the level to the administation of vitamin B would constitute a therapeutic test for the presence or otherwise of a deficiency of the vitamin. Lu (1939) found no abnormality of the resting blood pyruvic acid in cases of the 'atrophic type ' 15 16 ARCHIVES OF DISEASE IN CHILDHOOD of beriberi, which presented only nervous manifestations, although after exercise the raised level retuned to normal more slowly than in healthy individuals (Lu and Platt, 1939). Bueding et al (1941) reported that when glucose was given to thiamine-deficient subjects, the blood pyruvic acid rose to a higher level and remained elevated for a longer period than was the case with controls.…”

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Further Observations on the Significance of the Blood Pyruvic Acid Level in Infancy

Allibone¹

1948

Archives of Disease in Childhood

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“…of blood. Lu (1939b) Adults Davis and Bauer (1944) Adults The isolation of pyruvic acid from the blood of vitamin B1 deficient animals was first reported by Johnson (1936). Platt and Lu (1939) found the blood pyruvic acid to be raised in acute beri-beri.…”

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confidence: 99%