Lipoxidase demonstrated a typical protein inactivation response to heat as a function of pH when heated at 65°C and 0.1 molar buffer. The enzyme was found to be very sensitive to ionizing energy when irradiated in pH 7 buffer. This sensitivity was shown by a relatively low D, value (9 × 104 rad) and a rapid loss of enzyme activity during post‐irradiation storage. The results of combined treatment of the enzyme, in buffer, by heat and radiation showed that treatment order was important. Heating prior to…
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