Studies on the Catabolism of the Hydroxylysine-Linked Disaccharide Units of Basement Membranes and Collagens

Sternberg, M.; Spiro, Robert G.

doi:10.1159/000172732

Cited by 9 publications

(14 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An age-dependent increase in ␤-galactosidase activity (at pH 6) has been reported (97), and a cell surface inactive ␤-galactosidase functions as an elastin and laminin receptor (98). An ␣-glucosidase that removes glucose from (Glc-Gal)Hyl has been characterized (99). However, we found no evidence that deglycosylation could be performed extracellularly, because triple-helical glycopeptides were not substrates for purified ␤-galactosidase or melanoma cells.…”

Section: Discussionmentioning

confidence: 62%

Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen

Stawikowski

Aukszi

Stawikowska

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 62%

Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen

Stawikowski

Aukszi

Stawikowska

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The complement, activated by the surgical procedure (Dvorak et al 1988), causes an increase in Clq, the degradation of which produces GGHyl (Krane et al 1977). In rats, GGHyl can in turn be transformed into GHyl by a kidney a-glucosidase (Sternberg & Spiro 1979). Indeed, the excretory patterns of GHyl and GGHyl are parallel, highly correlated and insensitive to the bone remodeling inhibition induced by estrogen administration, thus supporting the hypothesis that both glycosides are of extra-osseous origin.…”

Section: Discussionmentioning

confidence: 87%

“…The GHyl to GGHyl ratio varies depending on different tissues. In rats, bone contains higher levels of GGHyl than GHyl (Michalsky et al 1993) and a specific a-glucosidase in the kidney can convert the glucosyl fraction into the galactosyl one (Sternberg & Spiro 1979), so that both urinary GHyl and GGHyl should be measured when they are being used as indices of bone résorption. In contrast with pyridinium cross-links (Black et al 1989, Tordjman et al 1994, urinary glycosides have not yet been vali¬ dated as markers of increased bone résorption induced by estrogen deficiency.…”

Section: Introductionmentioning

confidence: 99%

Comparison between urinary pyridinium cross-links and hydroxylysine glycosides in monitoring the effects of ovariectomy and 17β-estradiol replacement in aged rats

Pecile

Netti

Sibilia

et al. 1996

Journal of Endocrinology

View full text Add to dashboard Cite

This study was undertaken to assess the sensitivity of hydroxylysylpyridinoline (HP), lysylpyridinoline (LP), galactosylhydroxylysine (GHyl) and glucosylgalactosylhydroxylysine (GGHyl) to monitor bone response to estrogen deficiency and replacement by comparing their excretory patterns in ovariectomized aged (11-14 months old) rats. The ovariectomized (OVX) rats were randomized into two groups: (1) OVX plus vehicle; (2) OVX plus 17 beta-estradiol (17-beta E, 10 micrograms/kg, s.c., 4 days/week). Treatment with 17-beta E started immediately after OVX and continued for 60 days. The collagen catabolites were measured in urine for 1 month before OVX and thereafter for 60 days. In temporal coincidence with urine collection, bone area and bone mineral density (BMD) of lumbar vertebrae, femoral diaphysis and distal metaphysis were measured by dual-energy X-ray absorptiometry. In the untreated rats, BMD of the femoral metaphysis and lumbar vertebrae decreased significantly and the urinary excretion of LP, HP, GHyl and GGHyl increased with different patterns. In the treated rats, 17-beta E replacement prevented the increment in LP excretion, partially prevented the increase in HP excretion, but had no effect on the excretion of GHyl and GGHyl. In conclusion pyridinolines and glycosides have different sensitivities to the bone response to OVX. Glycoside excretion after OVX also reflects metabolic processes not strictly related to bone loss and, in contrast with LP, is not sensitive to estrogen replacement.

show abstract

“…In addition, there is the possibility that the decrease of both glycosides could be ascribed, at least in part, to clodronate inhibition of intracellular degradation of newly synthetised collagen (30) since GGHyl and GHyl might also derive from osteoblast synthetic activity and osteoblast intracellular degradation (3,29,31). Although rat bone contains more GGHyl than GHyl (32), the parallel changes of urinary glycosides are not unexpected since rat kidney cortex displays a specific a-glucosidase which converts the glucosyl fraction into the galactosyl form (33). However, we cannot exclude the possibilty that the reduction in urinary glycosides induced by clodronate might also be due to bisphosphonate effects on extraosseous sources of GGHyl e.g.…”

Section: Discussionmentioning

confidence: 99%

Responsiveness of urinary markers of bone resorption to orchiectomy and clodronate treatment in mature rats: a comparative study

Rubinacci¹,

Villa

Sibilia³

et al. 1998

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: The aim of this study was to assess the responsiveness of two classes of bone resorption markers to the enhancement of osteoclastic activity induced by orchiectomy and to its inhibition by clodronate treatment in mature rats. Design: Bone mineral density (BMD) at femural metaphysis, femural diaphysis, lumbar vertebrae, and the urinary excretion of pyridinoline (Pyr), deoxypyridinoline (D-Pyr), galactosylhydroxylysine (GHyl) and glucosylgalactosylhydroxylysine (GGHyl) were monitored at regular intervals for 30 days prior to and for 60 days following orchiectomy in eleven rats, divided into two groups: five rats untreated and the other six treated with clodronate. Results: Prior to orchiectomy, a significant (P < 0.01) decrease in BMD was observed only at the distal femural metaphysis. This decrease appeared to be associated with a time-dependent increase in the urinary excretion of all markers. Following orchiectomy, the BMD of the untreated group decreased significantly (P < 0.01) at all bone sites. The bone loss was accompanied by a significant (P < 0.01) increase in Pyr and D-Pyr concentrations in urine, whereas urinary GHyl and GGHyl did not change significantly. In the clodronate-treated group, the BMD of the three skeletal sites did not change significantly, while the urinary excretion of all urinary biochemical markers decreased significantly (P < 0.001). Conclusion: This study showed that pyridinolines are able to monitor the bone response to orchiectomy and to clodronate treatment response in androgen-deficient mature male rat, whereas glycosides appear prone to confounding factors.

show abstract

Studies on the Catabolism of the Hydroxylysine-Linked Disaccharide Units of Basement Membranes and Collagens

Cited by 9 publications

References 1 publication

Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen

Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen

Comparison between urinary pyridinium cross-links and hydroxylysine glycosides in monitoring the effects of ovariectomy and 17β-estradiol replacement in aged rats

Responsiveness of urinary markers of bone resorption to orchiectomy and clodronate treatment in mature rats: a comparative study

Contact Info

Product

Resources

About