Studies on the biology of fish bone—II bone matrix changes during resorption

Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones

“…The type B cell populates the demineralized zone which is packed with collagen fibers, devoid of crystals. This agrees with the chemical data of an increased percentage of organic material in resorbed bone [12,14]. It appears that the net collagen content of bone does not change during resorption as evidenced by both ultrastructural observations and amino acid analysis [12][13][14].…”

“…In mammalian bone resorption via osteocytes (osteolysis), the mineral is removed before the matrix [57,58]. In our previous study of fish bone resorption, carbohydrate decrease and protein change occurred prior to mineral removal [12,14]. The present electron micrographs give some clue as to the sequence of events, namely, possible ground substance and mineral removal before collagen followed by collagenolysis.…”

“…It appears that these cells remove ground substance from the osseous zone. This correlates with the decrease in carbohydrate observed in the soluble component of the organic matrix [12,14]. Eventually the type A cell further invades the osseous zone for further demineralization.…”

“…Also, our previous studies [12][13][14] indicated changes in organic matrix and bone resorption did take place in these fish after scale removal. The bone weight did not decrease after scale removal although calcium decreased by 75% (Table 1).…”

Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones

“…The type B cell populates the demineralized zone which is packed with collagen fibers, devoid of crystals. This agrees with the chemical data of an increased percentage of organic material in resorbed bone [12,14]. It appears that the net collagen content of bone does not change during resorption as evidenced by both ultrastructural observations and amino acid analysis [12][13][14].…”

“…In mammalian bone resorption via osteocytes (osteolysis), the mineral is removed before the matrix [57,58]. In our previous study of fish bone resorption, carbohydrate decrease and protein change occurred prior to mineral removal [12,14]. The present electron micrographs give some clue as to the sequence of events, namely, possible ground substance and mineral removal before collagen followed by collagenolysis.…”

“…It appears that these cells remove ground substance from the osseous zone. This correlates with the decrease in carbohydrate observed in the soluble component of the organic matrix [12,14]. Eventually the type A cell further invades the osseous zone for further demineralization.…”

“…Also, our previous studies [12][13][14] indicated changes in organic matrix and bone resorption did take place in these fish after scale removal. The bone weight did not decrease after scale removal although calcium decreased by 75% (Table 1).…”

Effects of skeletal element identity, delipidation and demineralization on the analysis of stable isotope ratios of C and N in fish bone

“…If acid soluble proteins represented a significant proportion of total protein in bulk fish bone and were lost during demineralization, the δ 15 N value of demineralized bone would be that of collagen and the δ 15 N value of untreated bone would be that of total protein. Published information is scarce, but available data suggest that non‐collagenous proteins may represent up to 16% of organic matrix of cortical bone (Weiss & Watabe, ). As the δ 15 N value of collagen may differ from that of other proteins the loss of acid soluble proteins may modify δ 15 N dml compared with δ 15 N bulk .…”

Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones

“…The type B cell populates the demineralized zone which is packed with collagen fibers, devoid of crystals. This agrees with the chemical data of an increased percentage of organic material in resorbed bone [12,14]. It appears that the net collagen content of bone does not change during resorption as evidenced by both ultrastructural observations and amino acid analysis [12][13][14].…”

“…In mammalian bone resorption via osteocytes (osteolysis), the mineral is removed before the matrix [57,58]. In our previous study of fish bone resorption, carbohydrate decrease and protein change occurred prior to mineral removal [12,14]. The present electron micrographs give some clue as to the sequence of events, namely, possible ground substance and mineral removal before collagen followed by collagenolysis.…”

“…It appears that these cells remove ground substance from the osseous zone. This correlates with the decrease in carbohydrate observed in the soluble component of the organic matrix [12,14]. Eventually the type A cell further invades the osseous zone for further demineralization.…”

“…Also, our previous studies [12][13][14] indicated changes in organic matrix and bone resorption did take place in these fish after scale removal. The bone weight did not decrease after scale removal although calcium decreased by 75% (Table 1).…”

Effects of skeletal element identity, delipidation and demineralization on the analysis of stable isotope ratios of C and N in fish bone

“…If acid soluble proteins represented a significant proportion of total protein in bulk fish bone and were lost during demineralization, the δ 15 N value of demineralized bone would be that of collagen and the δ 15 N value of untreated bone would be that of total protein. Published information is scarce, but available data suggest that non‐collagenous proteins may represent up to 16% of organic matrix of cortical bone (Weiss & Watabe, ). As the δ 15 N value of collagen may differ from that of other proteins the loss of acid soluble proteins may modify δ 15 N dml compared with δ 15 N bulk .…”

Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones

“…The type B cell populates the demineralized zone which is packed with collagen fibers, devoid of crystals. This agrees with the chemical data of an increased percentage of organic material in resorbed bone [12,14]. It appears that the net collagen content of bone does not change during resorption as evidenced by both ultrastructural observations and amino acid analysis [12][13][14].…”

“…In mammalian bone resorption via osteocytes (osteolysis), the mineral is removed before the matrix [57,58]. In our previous study of fish bone resorption, carbohydrate decrease and protein change occurred prior to mineral removal [12,14]. The present electron micrographs give some clue as to the sequence of events, namely, possible ground substance and mineral removal before collagen followed by collagenolysis.…”

“…It appears that these cells remove ground substance from the osseous zone. This correlates with the decrease in carbohydrate observed in the soluble component of the organic matrix [12,14]. Eventually the type A cell further invades the osseous zone for further demineralization.…”

“…Also, our previous studies [12][13][14] indicated changes in organic matrix and bone resorption did take place in these fish after scale removal. The bone weight did not decrease after scale removal although calcium decreased by 75% (Table 1).…”

Effects of skeletal element identity, delipidation and demineralization on the analysis of stable isotope ratios of C and N in fish bone

“…If acid soluble proteins represented a significant proportion of total protein in bulk fish bone and were lost during demineralization, the δ 15 N value of demineralized bone would be that of collagen and the δ 15 N value of untreated bone would be that of total protein. Published information is scarce, but available data suggest that non‐collagenous proteins may represent up to 16% of organic matrix of cortical bone (Weiss & Watabe, ). As the δ 15 N value of collagen may differ from that of other proteins the loss of acid soluble proteins may modify δ 15 N dml compared with δ 15 N bulk .…”