Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminata , a threatened orchid of nutraceutical importance

Bose, Biswajit; Choudhury, Hiranjit; Tandon, Pramod; Kumaria, Suman

doi:10.1016/j.jphotobiol.2017.07.010

Cited by 88 publications

(77 citation statements)

References 79 publications

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“…To date, studies have shown that the roles of phytochemicals could be exploited in dermatological products, mainly to prevent the occurrence of skin cancers and other dermatological pathologies promoted by the sun and/or free radicals (Bosch et al 2015;Dzialo et al 2016;Martins et al 2016;Bose et al 2017;Andrade et al 2019).…”

Section: Synergism Between Natural Products and Synthetic Sunscreensmentioning

confidence: 99%

Can natural products improve skin photoprotection?

et al. 2020

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Due to increased UV radiation on the Earth’s surface, caused by depletion of the stratospheric ozone, people have become more susceptible to different types of skin damage, such as erythema, sunburns, and cancer; this is especially of concern in tropical countries. Thus, efforts to improve awareness as well as the use of sunscreen are increasing worldwide. However, synthetic UV filters have been associated with deleterious effects such as photosensitization. Natural products have been used by ancient cultures for several purposes, including protecting the skin from the sun. However, there is still doubt today whether photoprotection is a real phenomenom or whether it is simply tanning of the skin. Plants have self-protective mechanisms and produce secondary metabolites that can protect themselves from UV radiation. Yet, can phytochemical compounds protect human skin? This review discusses the paradoxical effect of chemical UV filters and the influence of phytochemicals in in vitro and in vivo tests of photoprotection.

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Section: Synergism Between Natural Products and Synthetic Sunscreensmentioning

confidence: 99%

Can natural products improve skin photoprotection?

et al. 2020

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“…Collagenase breaks down collagen, and thus, inhibiting collagenase may be a meaningful way to prevent wrinkles. These enzymes can be measured chemically and directly . Tyrosinase is an oxidase that controls the production of melanin, which protects keratinocytes from ultraviolet ray (UV)–induced damage that leads to skin cancer, but also underlies UV‐induced senile pigment spots .…”

Section: Skin Ageing Researchmentioning

confidence: 99%

Alternative test models for skin ageing research

Nakamura

Haarmann‐Stemmann

Krutmann

et al. 2018

Experimental Dermatology

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Increasing ethical concerns regarding animal experimentation have led to the development of various alternative methods based on the 3Rs (Refinement, Reduction and Replacement), first described by Russell and Burch in 1959. Cosmetic research and skin ageing research are particularly susceptible to concerns related to animal testing. In addition to animal welfare reasons, there are scientific and economic reasons to reduce and avoid animal experiments. Importantly, animal experiments may not reflect findings in humans mainly because of the differences in architectures and immune responses between animal skin and human skin. Here, we review the shift from animal testing to the development and application of alternative non-animal-based methods and the necessity and benefits of this shift. Some specific alternatives to animal models are discussed, including biochemical approaches, 2-dimensional and 3-dimensional cell cultures and volunteer studies, as well as future directions, including genome-based research and the development of in silico computer simulations of skin models. Among the in vitro methods, 3-dimensional reconstructed skin models are highly popular and useful alternatives to animal models however still have many limitations. With careful selection and skilful handling, these alternative methods will become indispensable for modern dermatology and skin ageing research.

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“…Earlier studies proved that the efficient HAase's inhibitory ability of some flavonoids, like apigenin, luteolin, quercetin, kaempferol, and rutin is due to their closely related structure [17], on the other hand the activity towards Hyal increases with the increasing the number of hydroxyl groups, especially in the 2,3 position (quercetin) and 5 (myricetin) and decreased after glycosylation or substitution of hydroxyl groups [13,14]. Also, natural extracts such as brown seaweed (Padina pavonica) have shown an inhibitory effect on hyaluronidase and is used as an anti-aging product [18]. The methanolic extracts of Malaxis acuminate leaves and stems exhibited photo protective activity against UV-A and UV-B radiation in addition to acting as HAase inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Bioassay-Guided Isolation, Metabolic Profiling, and Docking Studies of Hyaluronidase Inhibitors from Ravenala madagascariensis

Mohamed¹,

Hetta

Rateb

et al. 2020

Molecules

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Hyaluronidase enzyme (HAase) has a role in the dissolution or disintegration of hyaluronic acid (HA) and in maintaining the heathy state of skin. Bioassay-guided fractionation of Ravenala madagascariensis (Sonn.) organ extracts (leaf, flower, stem, and root) testing for hyaluronidase inhibition was performed followed by metabolic profiling using LC–HRMS. Additionally, a hyaluronidase docking study was achieved using Molecular Operating Environment (MOE). Results showed that the crude hydroalcoholic (70% EtOH) extract of the leaves as well as its n-butanol (n-BuOH) partition showed higher HAase activity with 64.3% inhibition. Metabolic analysis of R. madagascariensis resulted in the identification of 19 phenolic compounds ranging from different chemical classes (flavone glycosides, flavonol glycosides, and flavanol aglycones). Bioassay-guided purification of the leaf n-BuOH partition led to the isolation of seven compounds that were identified as narcissin, rutin, epiafzelechin, epicatechin, isorhamnetin 7-O-glucoside, kaempferol, and isorhamnetin-7-O-rutinoside. The docking study showed that narcissin, rutin, and quercetin 3-O-glucoside all interact with HAase through hydrogen bonding with the Asp111, Gln271, and/or Glu113 residues. Our results highlight Ravenala madagascariensis and its flavonoids as promising hyaluronidase inhibitors in natural cosmetology preparations for skin care.

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Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminata , a threatened orchid of nutraceutical importance

Cited by 88 publications

References 79 publications

Can natural products improve skin photoprotection?

Can natural products improve skin photoprotection?

Alternative test models for skin ageing research

Bioassay-Guided Isolation, Metabolic Profiling, and Docking Studies of Hyaluronidase Inhibitors from Ravenala madagascariensis

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