Studies on Renal Tubular Transport

Koishi, Tetsuo

doi:10.1254/jjp.8.101

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…2). The values of K , measured in the present work at 37" agree well with those measured at 25" in the kidney cortical slice of the rabbit (6) and the rat ( 5 ) . On the other hand, acetate significantly increased V,,, in the present work.…”

Section: ' = ( 7 )supporting

confidence: 87%

Acetate Influence upon the Transport Kinetics of p-Aminohippurate at 37 C in Rabbit Kidney Slices

Gerencser

Chaisetseree

Hong

1977

Experimental Biology and Medicine

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Acetate has a stimulatory effect on the renal cortical p-aminohippurate transport system in vitro (1) and in vivo (2). This effect was first interpreted as a direct effect on metabolism related to the transport system (1-3). In fact, Cross and Taggart (1) suggested that the stimulation of p-aminohippurate accumulation seen in adult kidneys indicates that acetate is one of the ratelimiting cellular components involved in the transport system. On the other hand, Schachter et al. (4) suggested that acetate enhances p-aminohippurate uptake by its conjugation with glycine to form acetylglycine, which functions to relieve a normal degree of competitive inhibition by long chain acylglycines . The suggested relative specificity rendered by acetate (1-4) as a stimulatory agent was challenged by Koishi ( 5 ) who showed that acetate inhibited, as well as stimulated, p-aminohippurate uptake, and that pyruvate, succinate, and aketoglutarate quantitatively mimicked the effects elicited by acetate. The controversy concerning the role of acetate in the active uptake of weak organic acids by the kidney prompted us to perform comparable studies using p-aminohippurate as a model weak organic acid in order to elucidate the specific function of acetate.Material and methods. Adult New Zealand rabbits weighing from 2.5 to 4.0 kg were used in this investigation. After the rabbits were sacrificed by injecting air through an auricular vein, the kidneys were immediately removed and placed in an icecold (0") low-Na (10 mM) Cross-Taggart medium devoid of acetate. Renal cortical slices, approximately 0.3-0.5 mm thick and weighing 200-300 mg, were cut with a Stadie-Riggs tissue slicer and then were randomly divided into two groups. One group was transferred to a modified low-Na Cross-Taggart preincubation medium containing 10 mM acetate, whereas the other was transferred to a similar preincubation medium devoid of acetate. Preincubation was done at 0" for 30 min. The groups of slices were incubated in their appropriate high-Na (100 mM) modified Cross-Taggart medium containing p-aminohippurate, in either the presence or absence of 10 mM acetate, respectively. The compositions of the 10 and 100 mM Na media have previously been described (6). In the nonacetate series, sodium acetate was replaced by an equimolar amount of NaCl. All preincubations and incubations were performed under 100% 0,. The incubations were done in a Dubnoff metabolic shaker at either 25 or 37" for 45 min.Three different types of experiments were performed. In the first series of experiments, the effect of acetate on p-aminohippurate accumulation and oxygen consumption at both 25 and 37" was examined. Groups of slices were preincubated and incubated in either the presence or absence of acetate. In the accumulation experiments, the slices were blotted and weighed at the end of the incubations; the calculations were based upon this wet weight. In the oxygen consumption experiments, the slices were blotted and weighed prior to incubation. Oxygen uptake was represented on the bas...

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Section: ' = ( 7 )supporting

confidence: 87%

Acetate Influence upon the Transport Kinetics of p-Aminohippurate at 37 C in Rabbit Kidney Slices

Gerencser

Chaisetseree

Hong

1977

Experimental Biology and Medicine

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“…And the intercept on the 1. R axis appears to be unity, as was seen .%-ith malonate (1). And the inhibitory action is seen to be greater than that of malonate.…”

Section: Methodsmentioning

confidence: 51%

“…The procedures were the same as those described in the previous report (1), that is, they followed those of Cross & Taggart (2) in the main, the Warburg apparatus being used, and the contents of its main compartment was prepared according to the directions of Cross & Taggart. Sodium fluoride, the extent of which was taken to give the final concentration, was added previously to the solution of Cross & Taggart, and poured into the main compart ment.…”

Section: Methodsmentioning

confidence: 99%

“…In the previous report (1), the effect of certain members of citric acid cycle and related reagents on the accumulation of para-aminohippurate (briefly PAH) was observed and an enzymological analysis of the data was undertaken. It was recognized, there, that the ac cumulation was stimulated as well as inhibited by those members and it was suggested that succinic dehydrogenase might enter in mechanism of the accumulation.…”

Section: Introductionmentioning

confidence: 99%

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Studies on Renal Tubular Transport

Koishi

1959

Japanese Journal of Pharmacology

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“…A number of organic anions involved in intermediary metabolism such as lactate, pyruvate and acetate have been shown to stimulate, and in some cases inhibit, hippurate transport in kidney slices, depending on the concentration used (8,13). At the present time it is not possible to determine the relative importance of these metabolizable anions versus the role of nonmetabolized anions such as hippurate in altering renal anionic transport.…”

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confidence: 97%