Studies on Genetic Diversity of Twelve Accessions of Momordica charantia L. using Morphological, RAPD and SCAR Markers

Paul, A.; Bandyopadhyay, S.; Acharyya, Pinaki; Raychaudhuri, Sarmistha Sen

doi:10.3923/ajps.2010.471.478

Cited by 13 publications

(6 citation statements)

References 14 publications

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“…Relationship among accession could be explored by quantitative morphological characters (Chernet, Belew, & Abay, 2014). With the improvement of DNA technology, assessment of genetic relationship have been done by many molecular markers (Biswas, Akhond, Al-Amin, Khatun, & Kabir, 2009;Chen et al, 2009;Pu et al, 2009;Comlekcioglu, Simsek, Boncuk, & Aka-Kacar, 2010;Meng, Xu, Huang, & Li, 2010;Reck-Kortmann, Rodrigues, Ruas, & Freitas, 2013;Sharifova, Mehdiyeva, Theodorikas, & Roubos, 2013;Tabassum, Sony, Copyright © 2017 Universitas Brawijaya Bhajan, & Islam, 2013;Tasma, Warsun, Satyawan, Syafaruddin, & Martono, 2013;Aida & Eltayeb, 2015) Other researchers used the combination of both morphology and molecular makers (Paul, Bandyophadyay, Acharyya, & Raychaudhuri, 2010;Naz, Zafrullah, Shahzadhi, & Munir, 2013).…”

Section: Molecular Analysismentioning

confidence: 99%

“…Molecular markers that frequently used to assess genetic diversity are random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. RAPD markers are more effective and efficient to determine the recombinant variability than morphological markers in assessing genetic diversity (Paul, Bandyophadyay, Acharyya, & Raychaudhuri, 2010;Hapsoro, Warganegara, Utomo, Sriyani, & Yusnita). RAPD markers also become a potential tool to identify genetic differences among varieties (Biswas, Akhond, Al-Amin, Khatun, & Kabir, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Herison

Sutjahjo

Sulastrini³

et al. 2018

Agrivita.J.Agr.Sci

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Genetic diversity is the most important aspect in tomato breeding activities. Better assessment on the diversity of the collected accessions will come up with better result of the cultivar development. This study aimed at analyzing the genetic diversity of 27 tomato accessions by morphological and molecular markers. Twenty seven accessions collected from various regions of Indonesia were planted in the field and evaluated for their morphological traits, and RAPD analyzed for their molecular markers. The UPGMA clustering analyzes, elaborating the combination of morphological and molecular data, indicated that the tomato accessions could be grouped into 5 major groups with 70 % genetic similarity levels. Current study indicated that although many accessions came from different locations, they congregated into the same group. Cherry, Kudamati 1 and Lombok 3 were the farthest genetic distant accessions to the others. Those three genotypes will be the most valuable accessions, when they were crossed with other accessions, for designing a prospective breeding program in the future.

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Section: Molecular Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Herison

Sutjahjo

Sulastrini³

et al. 2018

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

show abstract

“…In contrary, F 2 population is the simplest and easiest to develop which is primarily based on Mendelian laws and therefore it was widely used for early genetic mapping and QTL analysis 27 – 29 , especially in non-model species with limited genetic research 30 . The genetic diversity analysis was reported earlier by many workers using various multi-locus dominant DNA markers such as RAPD 31 , 32 , ISSR 33 , and AFLP 34 in bitter gourd. The microsatellites i.e.SSR markers are mostly preferred because of their co-dominance, repeatability and easy transferability even though the initial cost of development of these markers is very high 35 , 36 .…”

Section: Introductionmentioning

confidence: 86%

Genetic analysis and QTL mapping of yield and fruit traits in bitter gourd (Momordica charantia L.)

Rao

Behera

Gaikwad

et al. 2021

Sci Rep

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Bitter gourd (Momordica charantia L.) is an economically important vegetable crop grown in tropical parts of the world. In this study, a high-density linkage map of M. charantia was constructed through genotyping-by-sequencing (GBS) technology using F2:3 mapping population generated from the cross DBGy-201 × Pusa Do Mausami. About 2013 high-quality SNPs were assigned on a total of 20 linkage groups (LGs) spanning over 2329.2 CM with an average genetic distance of 1.16 CM. QTL analysis was performed for six major yield-contributing traits such as fruit length, fruit diameter, fruit weight, fruit flesh thickness, number of fruits per plant and yield per plant. These six quantitative traits were mapped with 19 QTLs (9 QTLs with LOD > 3) using composite interval mapping (CIM). Among 19 QTLs, 12 QTLs derived from ‘Pusa Do Mausami’ revealed a negative additive effect when its allele increased trait score whereas 7 QTLs derived from ‘DBGy-201’ revealed a positive additive effect when its allele trait score increased. The phenotypic variation (R2%) elucidated by these QTLs ranged from 0.09% (fruit flesh thickness) on LG 14 to 32.65% (fruit diameter) on LG 16 and a total of six major QTLs detected. Most QTLs detected in the present study were located relatively very close, maybe due to the high correlation among the traits. This information will serve as a significant basis for marker-assisted selection and molecular breeding in bitter gourd crop improvement.

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“…The Momordica charantia decrease the genotoxic activity of methyl-nitrosamine, methanesulfonate and tetracycline, as shown by the decrease in chromosome breakage 129,83 .…”

Section: Anti-genotoxicmentioning

confidence: 98%

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2021

IJPSR

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Momordica charantia and Momordica dioica species belong to the Cucurbitaceae family. Momordica charantia and Momordica dioica climbing creeper plants are used both in the prevention and cure of various diseases and in the food of humans. Momordica charantia is also known as bitter gourds, bitter melon, karela, and balsam pear. Momordica charantia contain phytochemical constituents such as alkaloids, tannins, flavonoids, saponins, glycosides, steroids, phenolic compounds, terpenoids, proteins, mucilages and oleanolic acids as well as moisture, ash, carbohydrates, minerals and vitamins used as nutritional significance. It has been used in the treatment of tumor, inflammation, antioxidant, diabetes, hyperglycemic, hyperlipidemic, hepatoprotective, obesity, cancer, viral, viral infections (HIV, herpes, hepatitis, influenza and measles), bacterial infections, asthma, cough, skin disorder, eye diseases, immunomodulatory, microbial, cardiovascular, wound healing activities. Momordica dioica contains alkaloids, steroids, triterpenoids, flavonoids, glycosides, saponins, vitamins, protein, carbohydrates, momordicin. It has been used in the treatment of malaria, inflammation, antioxidant, diabetes, hyperglycemic. Both species are great potentiality from the economic and botanical points of view. This paper reviews the work done so far in the Literature Survey of plants Momordica charantia and Momordica dioica. INTRODUCTION: Momordica charantia andMomordica dioica belong to the family Cucurbitaceae 1 and under the genus Momordica, a genus of annual or perennial herbaceous climbers that contains about 80 species 2 . The plant Momordica charantia is also known as bitter gourds, bitter melon, karela, and balsam pear. These species include M. angustisepala, M.

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Studies on Genetic Diversity of Twelve Accessions of Momordica charantia L. using Morphological, RAPD and SCAR Markers

Cited by 13 publications

References 14 publications

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

Genetic analysis and QTL mapping of yield and fruit traits in bitter gourd (Momordica charantia L.)

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