Studies on Galactose Cataract Formation Utilizing Thymidine-Tritium

The proliferative ability of the lens epithelium in rat galactose cataracts. Examination using whole-mount preparations.

“…A paper which reported the proliferative ability of the lens epithelium during the development of the rat galactose cataracts using 3H-thymidine autoradiogaraphy on sections has previously been published (6). The writers created the galactose cataracts by feeding six-months-old rats with a diet containing 70% galactose and evaluated the DNA synthesizing ability of the lens epithelium.…”

The proliferative ability of the lens epithelium in rat galactose cataracts. Examination using whole-mount preparations.

“…A paper which reported the proliferative ability of the lens epithelium during the development of the rat galactose cataracts using 3H-thymidine autoradiogaraphy on sections has previously been published (6). The writers created the galactose cataracts by feeding six-months-old rats with a diet containing 70% galactose and evaluated the DNA synthesizing ability of the lens epithelium.…”

An Autoradiographic Study on Cell Migration in the Eye Lens Epithelium From Normal and Alloxan Diabetic Rats

“…As mitosis in the lens provides the force by which the epithelium cells are pushed towards the bow area an increased time of cell generation means a retardation of this migration, which corresponds to the results plotted in Figs. 2-4. In galactose cataract, cell migration is not increased in spite of a higher frequency of mitosis (6). Instead, a greatly increased number of lens epithelial cells are piled up under the anterior capsule.…”

Cell Growth of Rat Lens Epithelium in Galactose-induced Cataracts.

“…Cataracts that develop in rats fed a galactose supplemented diet are thought to be useful as an animal model of diabetic cataracts [7,13,15]. We believe that the proliferation potency at the central part of the lens is strongly related to maturity onset cataracts or diabetic cataracts, because this proliferation potency decreases with aging [5,6,12,16] and increases in some types of human cataracts or galactose induced cataract in rats [4,14], while lens epithelial cells in normal rats grow vigorously at the equator. In the present study, the proliferation potency of lens epithelial cells at the central part of the lens was studied in rats fed 5%-25% galactose supplemented diets in order to examine the relationship between cataract development and epithelial cells.…”

“…In general, while senile cataracts are caused by cortical changes such as swelling and liquefaction of lens fiber, subcapsular cataracts display abnormal growth and stratification of lens epithelial cells from the early stages of the disease, suggesting that the development of cataracts is profoundly influenced by epithelial cell growth. In previous studies [4,14] that attempted to elucidate the cause of abnormal growth of lens epithelial cells, we reported that, in rat galactose cataracts, which is an animal model of diabetic cataracts, repeated ectopic growth of the lens epithelial cells was observed in areas other than the equator in the early stage of cataract development [14]. In the present study, two different methods, fluorescence cytophotometry of 4',6-diamidino-2-phenylindole (DAPI) staining and immunohistochemical staining of proliferating cell nuclear antigen (PCNA), were used to study the growth kinetics of epithelial cells of the anterior pole of lenses in rats fed low concentration galactose diets that are unable to induce diabetic cataracts in the early stages.…”

The proliferative ability of the lens epithelium in rat galactose cataracts. Examination using whole-mount preparations.

“…A paper which reported the proliferative ability of the lens epithelium during the development of the rat galactose cataracts using 3H-thymidine autoradiogaraphy on sections has previously been published (6). The writers created the galactose cataracts by feeding six-months-old rats with a diet containing 70% galactose and evaluated the DNA synthesizing ability of the lens epithelium.…”

An Autoradiographic Study on Cell Migration in the Eye Lens Epithelium From Normal and Alloxan Diabetic Rats

“…As mitosis in the lens provides the force by which the epithelium cells are pushed towards the bow area an increased time of cell generation means a retardation of this migration, which corresponds to the results plotted in Figs. 2-4. In galactose cataract, cell migration is not increased in spite of a higher frequency of mitosis (6). Instead, a greatly increased number of lens epithelial cells are piled up under the anterior capsule.…”

Cell Growth of Rat Lens Epithelium in Galactose-induced Cataracts.

“…Cataracts that develop in rats fed a galactose supplemented diet are thought to be useful as an animal model of diabetic cataracts [7,13,15]. We believe that the proliferation potency at the central part of the lens is strongly related to maturity onset cataracts or diabetic cataracts, because this proliferation potency decreases with aging [5,6,12,16] and increases in some types of human cataracts or galactose induced cataract in rats [4,14], while lens epithelial cells in normal rats grow vigorously at the equator. In the present study, the proliferation potency of lens epithelial cells at the central part of the lens was studied in rats fed 5%-25% galactose supplemented diets in order to examine the relationship between cataract development and epithelial cells.…”

“…In general, while senile cataracts are caused by cortical changes such as swelling and liquefaction of lens fiber, subcapsular cataracts display abnormal growth and stratification of lens epithelial cells from the early stages of the disease, suggesting that the development of cataracts is profoundly influenced by epithelial cell growth. In previous studies [4,14] that attempted to elucidate the cause of abnormal growth of lens epithelial cells, we reported that, in rat galactose cataracts, which is an animal model of diabetic cataracts, repeated ectopic growth of the lens epithelial cells was observed in areas other than the equator in the early stage of cataract development [14]. In the present study, two different methods, fluorescence cytophotometry of 4',6-diamidino-2-phenylindole (DAPI) staining and immunohistochemical staining of proliferating cell nuclear antigen (PCNA), were used to study the growth kinetics of epithelial cells of the anterior pole of lenses in rats fed low concentration galactose diets that are unable to induce diabetic cataracts in the early stages.…”

The proliferative ability of the lens epithelium in rat galactose cataracts. Examination using whole-mount preparations.

“…A paper which reported the proliferative ability of the lens epithelium during the development of the rat galactose cataracts using 3H-thymidine autoradiogaraphy on sections has previously been published (6). The writers created the galactose cataracts by feeding six-months-old rats with a diet containing 70% galactose and evaluated the DNA synthesizing ability of the lens epithelium.…”

An Autoradiographic Study on Cell Migration in the Eye Lens Epithelium From Normal and Alloxan Diabetic Rats

“…As mitosis in the lens provides the force by which the epithelium cells are pushed towards the bow area an increased time of cell generation means a retardation of this migration, which corresponds to the results plotted in Figs. 2-4. In galactose cataract, cell migration is not increased in spite of a higher frequency of mitosis (6). Instead, a greatly increased number of lens epithelial cells are piled up under the anterior capsule.…”

Cell Growth of Rat Lens Epithelium in Galactose-induced Cataracts.

“…Cataracts that develop in rats fed a galactose supplemented diet are thought to be useful as an animal model of diabetic cataracts [7,13,15]. We believe that the proliferation potency at the central part of the lens is strongly related to maturity onset cataracts or diabetic cataracts, because this proliferation potency decreases with aging [5,6,12,16] and increases in some types of human cataracts or galactose induced cataract in rats [4,14], while lens epithelial cells in normal rats grow vigorously at the equator. In the present study, the proliferation potency of lens epithelial cells at the central part of the lens was studied in rats fed 5%-25% galactose supplemented diets in order to examine the relationship between cataract development and epithelial cells.…”

“…In general, while senile cataracts are caused by cortical changes such as swelling and liquefaction of lens fiber, subcapsular cataracts display abnormal growth and stratification of lens epithelial cells from the early stages of the disease, suggesting that the development of cataracts is profoundly influenced by epithelial cell growth. In previous studies [4,14] that attempted to elucidate the cause of abnormal growth of lens epithelial cells, we reported that, in rat galactose cataracts, which is an animal model of diabetic cataracts, repeated ectopic growth of the lens epithelial cells was observed in areas other than the equator in the early stage of cataract development [14]. In the present study, two different methods, fluorescence cytophotometry of 4',6-diamidino-2-phenylindole (DAPI) staining and immunohistochemical staining of proliferating cell nuclear antigen (PCNA), were used to study the growth kinetics of epithelial cells of the anterior pole of lenses in rats fed low concentration galactose diets that are unable to induce diabetic cataracts in the early stages.…”