Studies on enhancing embryo quantity and quality by immunization against inhibin in repeatedly superovulated Holstein heifers and the associated endocrine mechanisms

Liu, Y.P.; Mao, Xi-Guang; Wei, Yulong; Yu, Jianning; Li, H.; R.A.Chen,; Shi, Zhu

doi:10.1016/j.anireprosci.2013.08.005

Cited by 18 publications

(26 citation statements)

References 36 publications

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“…In vitro studies have demonstrated that treatment with an anti-inhibin α-subunit antibody improves the quality and development of cultured bovine or porcine granulosa cells [23][24][25], with substantial enhancements in E2 secretion, cell proliferation, and the expression levels of genes involved in follicle development and maturation [23]. In addition, the anti-inhibin α-subunit antibody treatment of in vitro-matured oocytes also improves the maturation rate and subsequently, the quality of early embryo development [21,22,26]. These in vivo and in vitro results indicated that immunization against inhibin could enhance ovarian follicle, oocyte, and early embryo development.…”

Section: Introductionmentioning

confidence: 99%

Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol

Guo

Chen

Cheng³

et al. 2020

Animals

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This study was conducted to investigate the feasibility of improving fertility in dairy cows via immunization against inhibin. Thirty-two cows were divided into Control (n = 11), Low-dose (n = 10) and High-dose (n = 11) groups. The High-dose and Low-dose cows were treated with 1 and 0.5 mg of the inhibin immunogen, respectively. All the cows were subjected to the Ovsynch protocol from the day of antigen administration and were artificially inseminated. Blood samples were serially collected over a 24-day period from the start of the Ovsynch protocol to 14 days after insemination. The results showed that immunization against inhibin dose-dependently increased the plasma concentrations of follicle-stimulating hormone (FSH), estradiol (E2), and activin A, but decreased progesterone (P4) concentrations in the luteal phase. Immunization also increased the plasma interferon (IFN)-τ concentrations in pregnant cows on day 14 after initial insemination. The conception rates in High-dose (45.5%) and Low-dose (40%) cows marginally increased compared to that in Control cows (27.3%), but the increases were not significant (p > 0.05). In conclusion, a single immunization against inhibin has the potential to improve conception rates, despite impaired luteal development. To further improve the reproductive performance of dairy cows, additional luteal-stimulating treatments are suggested in combination with immunization against inhibin and Ovsynch techniques.

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Section: Introductionmentioning

confidence: 99%

Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol

Guo

Chen

Cheng³

et al. 2020

Animals

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“…Immunization against inhibin negated the adverse effect of inhibin or its a‐subunit on oocyte developmental competence (Manjunatha et al., ) and elevated the activin concentration and the ratio of activin to follistatin in ovarian follicular fluid (Li et al., ) and plasma of immunized animals (Liu et al., ). Studies by Austin et al.…”

Section: Discussionmentioning

confidence: 99%

“…Immunization against inhibin negated the adverse effect of inhibin or its a-subunit on oocyte developmental competence (Manjunatha et al, 2007) and elevated the activin concentration and the ratio of activin to follistatin in ovarian follicular fluid (Li et al, 2011) and plasma of immunized animals (Liu et al, 2013). Studies by Austin et al (2001) showed that the increased activin to follistatin ratio in the large follicle could support the follicular further growth and enhance oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

Nasal immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis for improving ovarian responses and fertility in cross‐bred buffaloes

Liu

Rehman

et al. 2016

Reprod Domestic Animals

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This study was conducted to determine the effect of immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis on ovarian responses and fertility in cross-bred buffaloes. A total of 134 cross-bred buffaloes were divided into four groups: groups T1 (n = 34), T2 (n = 35) and T3 (n = 31) were nasal immunized twice a day with 10 ml of 1 × 10 CFU/ml of the C501 (pVAX-asd-IS) vaccine for 5, 3 and 1 day, respectively. Group C (n = 34) was nasal immunized with 10 ml PBS for 5 days. All animals were immunized twice with an interval of 14 days and administered with 200 μg of a GnRH analogue on day 28, 0.5 mg PGF on day 35 and 200 μg of the same GnRH analogue on day 37. TAI was performed at 18 and 24 hr after the second GnRH treatment. Fourteen days after primary immunization, C501 (pVAX-asd-IS) elicited significant immune responses, and anti-inhibin IgG antibody titres in group T1 were significantly higher (p < .01) than groups T3 and C. After the second GnRH treatment, the growth speed of the dominant follicles in group T1 was significantly faster (p < .05) than groups T3 and C. The number and diameter of large follicles (≥10 mm) as well as ovulatory follicles in group T1 were the greatest in all groups, resulting in a greater conception rate in buffaloes with positive anti-inhibin antibodies. These results demonstrate that immunization with the C501 (pVAX-asd-IS) vaccine, coupled with the Ovsynch protocol, could be used as an alternative approach to improve reproductive performance in cross-bred buffaloes.

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“…Superovulation treatments with exogenous gonadotropins result in increased numbers of developing follicles, which in turn lead to inhibin concentration rise in plasma [85] and a quantitatively and qualitatively reduced oocyte and embryo development [86]. Consequently, an immunization practice against inhibin, combined with a conventional superovulation protocol, has proven to enhance the quality of the resulting embryos both in vitro and in vivo [87–89]. This immunization has been achieved through the administration of exogenous inhibin leading to antibody production against this glycoprotein.…”

Section: Recombinant Hormones In Reproductionmentioning

confidence: 99%

“…However, in many other cases inhibin has been produced in mammalian cells [16, 92]. Importantly, recombinant inhibin has been used as an antigen for the immunization against endogenous inhibin in hens [93, 94], cockerels [95], heifers [87, 89, 96], water buffaloes [88], guinea pigs [97], goats [98], and sheep [99–102]. Only in a minority of cases, the recombinant inhibin α subunit used is obtained synthetically [103–106] or purified from follicular fluid [107].…”

Section: Recombinant Hormones In Reproductionmentioning

confidence: 99%

Trends in recombinant protein use in animal production

et al. 2017

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Recombinant technologies have made possible the production of a broad catalogue of proteins of interest, including those used for animal production. The most widely studied proteins for the animal sector are those with an important role in reproduction, feed efficiency, and health. Nowadays, mammalian cells and fungi are the preferred choice for recombinant production of hormones for reproductive purposes and fibrolytic enzymes to enhance animal performance, respectively. However, the development of low-cost products is a priority, particularly in livestock. The study of cell factories such as yeast and bacteria has notably increased in the last decades to make the new developed reproductive hormones and fibrolytic enzymes a real alternative to the marketed ones. Important efforts have also been invested to developing new recombinant strategies for prevention and therapy, including passive immunization and modulation of the immune system. This offers the possibility to reduce the use of antibiotics by controlling physiological processes and improve the efficacy of preventing infections. Thus, nowadays different recombinant fibrolytic enzymes, hormones, and therapeutic molecules with optimized properties have been successfully produced through cost-effective processes using microbial cell factories. However, despite the important achievements for reducing protein production expenses, alternative strategies to further reduce these costs are still required. In this context, it is necessary to make a giant leap towards the use of novel strategies, such as nanotechnology, that combined with recombinant technology would make recombinant molecules affordable for animal industry.

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Studies on enhancing embryo quantity and quality by immunization against inhibin in repeatedly superovulated Holstein heifers and the associated endocrine mechanisms

Cited by 18 publications

References 36 publications

Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol

Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol

Nasal immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis for improving ovarian responses and fertility in cross‐bred buffaloes

Trends in recombinant protein use in animal production

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