2017
DOI: 10.1371/journal.pone.0176701
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Abstract: The objective of this study was to investigate three storage methods and four storage times for rumen sampling in terms of quality and yield of extracted metagenomic DNA as well as the composition of the rumen bacterial community. One Nellore steer fitted with a ruminal silicone-type cannula was used as a donor of ruminal contents. The experiment comprised 11 experimental groups: pellet control (PC), lyophilized control (LC), P-20: pellet stored frozen at -20°C for a period of 3, 6, and 12 months, P-80: pellet… Show more

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Cited by 20 publications
(24 citation statements)
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“…Richness estimators and diversity indices showed that rumen bacterial microbiota is relatively rich and diverse and description of the community from the number of reads obtained was adequate. The Ace, Chao1, and Shannon values were higher than those observed by Granja-Salcedo et al . (2017) in one Nellore steer grazing on similar forage.…”
Section: Discussioncontrasting
confidence: 77%
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“…Richness estimators and diversity indices showed that rumen bacterial microbiota is relatively rich and diverse and description of the community from the number of reads obtained was adequate. The Ace, Chao1, and Shannon values were higher than those observed by Granja-Salcedo et al . (2017) in one Nellore steer grazing on similar forage.…”
Section: Discussioncontrasting
confidence: 77%
“…Sequencing on the Illumina platform allowed for the identification of 27 distinct phyla in the rumen contents, a higher number than that reported by Granja-Salcedo et al . (2017), Kim et al .…”
Section: Discussionmentioning
confidence: 83%
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