Studies of normal urinary proteins by gradient-SDS-PAGE and electrophoretic blotting.

Sakurai, Hideko; Sudou, Yuko; Inage, H; Watanabe, Kazuo; Kôyama, Akio; Narita, Mitsuharu; Tojo, Shizuo

doi:10.2198/sbk.30.251

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a further experiment, cytokeratins were also identified by a murine monoclonal anti-cytokeratin antibody (MNF-116 clone, DAKO) at a dilution of 1:500, followed by horse radish peroxidase-conjugated goat anti-mouse immunoglobulins (Cappel, Costa Mesa, CA) at a dilution of 1:500. The peroxidase reaction product was visualized using 4-chloro naphthol (Sakurai et al, 1986).…”

Section: Electron Microscopic Immunocytochemistrymentioning

confidence: 99%

Apolipoprotein E is Present in Primary Localized Cutaneous Amyloidosis

Furumoto¹,

Shimizu²,

Asagami³

et al. 1998

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Apolipoprotein E (apoE) is one of the amyloid associated proteins that is found in the amyloid plaque of Alzheimer's disease and systemic amyloidosis. ApoE might play an important part in the etiology of Alzheimer's disease by functioning as a "pathologic chaperone" to promote the formation of amyloid filaments. In this study, we investigated whether apoE is associated with amyloid deposits of primary localized cutaneous amyloidosis using immunohistochemistry, immunogold electron microscopy, and immunoblotting. The subjects consisted of 12 patients with lichen amyloidosus and one patient with macular amyloidosis. Light microscopically, amyloid deposits in the dermal papillae were round in shape and stained with Congo red. Immunohistochemically, apoE was detected in amyloid deposits in all the cases examined. Immunogold electron microscopy showed apoE immunoreactivity on the amyloid deposition. Immunoblots of amyloid-positive skin showed 35K and 14K proteins, which were taken to be apoE and its fragment, respectively. In normal skin extract, only the 35K protein was detected by the anti-human apoE. Moreover, the intensity of the amyloid-positive skin sample was stronger than that of the normal skin sample. Monoclonal anti-cytokeratin antibody reacted with the 45K protein of the amyloid-positive skin extract. These results indicate that apoE is a component of primary localized cutaneous amyloidosis, and that it might play an important role in primary localized cutaneous amyloidosis.

show abstract

Section: Electron Microscopic Immunocytochemistrymentioning

confidence: 99%