Studies of nonlinear electrical effects of model membranes

Carius, W.

doi:10.1007/bf00535705

Cited by 4 publications

(1 citation statement)

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“…where U is the voltage across the membrane core (intramembrane field), ␣ ϵ C 0 /2h 0 k is the "compliance" of the elastic capacitor with the modulus k and the distance between the plates h 0 . When the voltage U ϭ U 0 ϩ U 1 sin t is applied to the elastic capacitor, the current j ϭ dCU/dt has three harmonics (Carius, 1977;Hianik and Passechnik, 1995),…”

Section: Boundary Potential: the Intramembrane Field Compensation Methodsmentioning

confidence: 99%

Dipole Potentials Indicate Restructuring of the Membrane Interface Induced by Gadolinium and Beryllium Ions

Ermakov

Averbakh

Yusipovich

et al. 2001

Biophysical Journal

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The dipole component of the membrane boundary potential, phi(d), is an integral parameter that may report on the conformational state of the lipid headgroups and their hydration. In this work, we describe an experimental approach to measurements of the dipole potential changes, Deltaphi(d), and apply it in studies of Be(2+) and Gd(3+) interactions with membranes composed of phosphatidylserine (PS), phosphatidylcholine (PC), and their mixtures. Deltaphi(d) is determined as the difference between the changes of the total boundary potential, phi(b), measured by the IFC method in planar lipid membranes and the surface potential, phi(s), determined from the electrophoretic mobility of liposomes. The Gouy-Chapman-Stern formalism, combined with the condition of mass balance, well describes the ion equilibria for these high-affinity cations. For the adsorption of Be(2+) and Gd(3+) to PC membranes, and of Mg(2+) to PS membranes, the values of Deltaphi(b) and Deltaphi(s) are the same, indicative of no change of phi(d). Binding of Gd(3+) to PS-containing membranes induces changes of phi(d) of opposite signs depending on the density of ionized PS headgroups in the bilayer. At low density, the induced Deltaphi(d) is negative (-30 mV), consistent with the effect of dehydration of the surface. At maximal density (pure PS, neutral pH), adsorption of Be(2+) or Gd(3+) induces an increase of phi(d) of 35 or 140 mV, respectively. The onset of the strong positive dipole effect on PS membranes with Gd(3+) is observed near the zero charge point and correlates with a six-fold increase of membrane tension. The observed phenomena may reflect concerted reorientation of dipole moments of PS headgroups as a result of ion adsorption and lipid condensation. Their possible implications to in-vivo effects of these high-affinity ions are discussed.

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Section: Boundary Potential: the Intramembrane Field Compensation Methodsmentioning

confidence: 99%