Structures of cell wall mannans of pathogenic Candida tropicalis IFO 0199 and IFO 1647 yeast strains

Abstract

We conducted a structural analysis of the cell wall mannans isolated from two Candida tropicalis strains, IFO 0199 and IFO 1647, exhibiting strong agglutinabilities against anti-Candida factor sera 5 and 6. The products released from these mannans by acid treatment were identified as the oligosaccharides, from biose to pentaose, consisting solely of "-1,2-linked mannopyranose units corresponding to common epitopes of Candida albicans serotypes A and B (factor 5). Mild acetolysis of acidand alkali-treated mannans produced large amounts of hexaose and heptaose, Manp1l-2Manpl-1anpotlol-2Manpol-2Manpal-2Man and Manpol-2Manpfrl-2Manpjl-2Manpal-2Manpft1-2Manppal-2Man, corresponding to the C. albicans serotype A-specific epitopes (factor 6). However, the homologous pentaose, Manpjl-2Manpal-2Manpal-2Manpyal-2Man, was not generated by this procedure. The oligosaccharides (biose to hexaose) obtained from the mannans by conventional acetolysis were composed exclusively of a-1,2-linked mannopyranose units. Therefore, the mannans of C. tropicalis IFO 0199 and IFO 1647 do not have the a-1,3-linked mannopyranose units previously observed in the mannans of C. albicans and Candida stelatoidea. The results of this study and previous findings indicate that the similarity of the antigenicities of three Candida species, C. albicans serotype A, C. steUatoidea type II, and C. tropicalis, reside in the 0-1,2 and ot-1,2 linkages containing oligomannosyl side chain (factor 6) in the cell wall mannan.

Definitive chemical evidence for the constitutive ability of Candida albicans serotype A strains to synthesize β‐1,2 linked oligomannosides containing up to 14 mannose residues

“…β‐1,2 linked mannosides are uncommon glycosidic structures whose presence has never been reported in mammals so far but which have been evidenced as dispersed linkages in complex heteropolymers of some bacterial species [23, 24]. These linkages are more common in yeasts and especially in the mannan of Candida species where they are present at the reducing end of acid‐stable α linked oligomannosides [9, 25–28] or as phosphate bound acid‐labile homopolymers [9, 29, 30]. These two classes of oligomers support respectively the specificity of the factor sera 6 and 5 [31, 32] from the Candida Check, a commercially available kit intended for the identification of medically important Candida species.…”

Another brick in the wall? Recent developments concerning the yeast cell envelope

“…This complexity is multiplied by secondary and tertiary structural considerations also implicit in the relatively simple, linear structures of proteins and nucleic acids. The wealth of variation in wall carbohydrates is increasingly being used to facilitate yeast taxonomy (Depree et a1.,1993;Prillinger et al, 1993;Kobayashi et al, 1994).…”

Determination of <i>α</i>-1,3-Linked Mannose Residue in the Cell Wall Mannan of <i>Candida tropicalis</i> NBRC 1400 Strain

“…Kobayashi et al [12] showed that cells of C. tropicalis IFO 0199, IFO 0589, IFO 1400 and IFO 1647 are clearly aggregated with FAbs 1, 4, 5, and 6, and they reported that it the same as those of C. albicans serotype A (J-1012 strain) cells.…”

“…These FAbs recognize the antigenic determinants in cell wall mannan [11]. We reported the structure of cell wall mannans of several Candida species, including C. tropicalis [12], C. albicans [13] [14], C. glabrata [15], C. guilliermondii [16], and C. krusei [17]. Moreover, we revealed that the determinants of antigenic factors 5, 6, and 9 correspond to the oligomannosyl side chains consisting of a homologous β-1,2-linked series [18], of β-1,2 and α-1,2-linked mannose residues [19], and of β-1,2 and α-1,3-linked mannose residues [20] [21], respectively.…”

“…Moreover, we revealed that the determinants of antigenic factors 5, 6, and 9 correspond to the oligomannosyl side chains consisting of a homologous β-1,2-linked series [18], of β-1,2 and α-1,2-linked mannose residues [19], and of β-1,2 and α-1,3-linked mannose residues [20] [21], respectively. On the other hand, the determinants of Candida factors 1, 4, 13b, and 34 are α-linked oligomannosyl side chains, a linear homologous α-1,2-linked series [12], a 3,6-branched series [22], an internal α-1,3-linkage-containing linear series [22] [23], respectively.…”