2008
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Abstract: Amelogenin is the predominant protein found during enamel development and has been shown to be essential to proper enamel formation. Leucine-rich amelogenin peptide (LRAP) is a naturally occurring splice variant that preserves the charged N-and C-termini of full length amelogenin, regions thought to be crucial in interacting with hydroxaypatite. Particularly, the highly charged Cterminal hexapeptide (KREEVD) is thought to be the region most intimately interacting with hydroxyapatite (HAP). The structure of thi… Show more

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“…39,40 These clusters co-assemble as chains of nanoparticles that evolve into long co-aligned crystals resembling those found in biological enamel. 41 NMR spectroscopy studies 34,42 and others 43,44 have provided direct evidence that the Amel C-terminus is close enough to the HAP surface to direct growth. Our previous studies 40,41 have concluded that hydrophilic C-termini toward the exterior, away from the primarily hydrophobic cores of a full-length Amel protein, 45 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 …”
Section: Resultsmentioning
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…39,40 These clusters co-assemble as chains of nanoparticles that evolve into long co-aligned crystals resembling those found in biological enamel. 41 NMR spectroscopy studies 34,42 and others 43,44 have provided direct evidence that the Amel C-terminus is close enough to the HAP surface to direct growth. Our previous studies 40,41 have concluded that hydrophilic C-termini toward the exterior, away from the primarily hydrophobic cores of a full-length Amel protein, 45 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 …”
Section: Resultsmentioning
“…33 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 have an important functional role, possibly allowing maximum crystal inhibition at low protein concentrations. 34 Tarasevich et al demonstrated that LRAP is primarily a monomer in saturated calcium phosphate solutions. 35 Moreover, the LRAP protein is not completely extended on the surface, having some degree of structure away from the surface by neutron reflectivity.…”
Section: Resultsmentioning
“…Other evidence of motion is described below. The observation of some protein mobility suggests that the interface between amelogenin and HAP in the lysine regions allows for flexibility, an observation made for regions of both LRAP and statherin bound to HAP (Shaw et al, 2000;Shaw and Ferris, 2008) that may be related to function, such as allowing a single protein to cover more growth sites or serving a second function, such as protein binding.…”
Section: Amelogenin On Hap (1d-nmr)mentioning
“…This would result if part of the amelogenin molecule interacts strongly with the HAP surface (for instance, the C-terminus), while the other part (for instance, the N-terminus) does not. Analysis of previous SSNMR data with LRAP showed that both the N-and C-termini interacted tightly with HAP surfaces, whether phosphorylated at S16 or not, although the N-terminal K24 residue was slightly farther from the surface (7.5 Å to 9.0 Å) and became less structured for the non-phosphorylated sample (Shaw et al, 2004;Shaw and Ferris, 2008;Masica et al, 2011). The recombinant sample reported here is also non-phosphorylated, and it is possible that a looser interaction of the N-terminus could result in a similar increase in mobility and loss of structure.…”
Section: Amelogenin On Hap (1d-nmr)mentioning
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