Structure of the Fat Body

Dean, R.L.; Locke, Michael

doi:10.1016/b978-0-08-030804-3.50011-x

Cited by 73 publications

(106 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result was verified by analyzing trophocyte images close to the salivary gland during the larval development of P. villosa (Zara, 1995). That the presence of these granules can only be noted during the last larval instar agrees with observations made by Locke (1984), Dean et al (1985), and Keeley (1985).…”

Section: Discussionsupporting

confidence: 91%

“…The presence of protein granules has been described for several insect orders, besides Hymenoptera, such as Coleoptera, Diptera, Lepidoptera, and Orthoptera (Dean et al, 1985). The function of these granules is related to the storage of proteins for metamorphosis, and the presence of these granules can be visualized during the last larval instar (Price, 1969;Locke, 1984;Dean et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…Besides proteins, the trophocytes also store lipids and glycogen which they themselves produce or sequester from hemolymph (Bishop, 1922;Price, 1969;Dean et al, 1985;Keely, 1985). Such compounds are present in P. villosa and they were identified through the histochemical tests of Sudan Black B and PAS, respectively.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Zara

Caetano

2004

Braz. J. Biol.

View full text Add to dashboard Cite

The fat body cells of Pachycondyla (=Neoponera) villosa are disposed in a single layer between the cuticle and the digestive tract, forming a group of cells enclosed by a thin membrane. Histological studies have revealed three different cellular types: trophocyte (more abundant), urate, (located among the trophocytes), and oenocyte (the scarcest), usually observed laying near the cuticle. Histochemically, the trophocytes showed a positive reaction for basic proteins in the nucleus and cytoplasm, as well as a strong positive reaction in the cytoplasmic granules. The test for carbohydrates showed a strong positive reaction throughout the cytoplasm, while the test for lipids was positive for the cytoplasmic vesicles. The urate cells showed a positive reaction for basic proteins in the nucleus and in the areas of the cytoplasm surrounding the vesicles. These cells did not react to the PAS test or to Sudan Black B. The oenocytes showed a weak positive reaction to PAS and a strong positive reaction to Sudan Black B and Mercuric-bromophenol Blue.Key words: Pachycondyla, fat body, histochemistry, histology, larva. RESUMO Ultramorfologia e histoquímica do corpo gorduroso de larvas de último estágio dePachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)As células do corpo gorduroso de Pachycondyla (=Neoponera) villosa distribuem-se como uma única camada entre a cutícula e o trato digestivo, formando um conjunto de células agrupadas e recobertas por uma fina membrana. Não foram identificados tipos celulares distintos por meio da ultramorfologia, porém a histologia revelou três tipos celulares distintos: os trofócitos, mais abundantes, as células de urato, distribuídas por entre os trofócitos, e os enócitos, menos abundantes que os demais. Os enócitos são comumente observados próximos da cutícula. Histoquimicamente, os trofócitos apresentaram reação positiva para proteínas básicas no núcleo e no citoplasma e reação fortemente positiva nos grânulos citoplasmáticos. O teste para carboidratos foi fortemente positivo em todo o citoplasma, enquanto para os lipídeos observouse reação positiva nas vesículas citoplasmáticas. Em relação às células de urato, estas apresentaram reação positiva para proteínas básicas no núcleo e citoplasma, por entre as vesículas. Essas células não apresentaram reação para o teste de PAS e Sudan Black B. Quanto aos enócitos, estes apresentaram citoplasma fracamente positivo ao PAS e fortemente positivo ao Sudan Black B e para o azul de bromofenol.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Zara

Caetano

2004

Braz. J. Biol.

View full text Add to dashboard Cite

show abstract

“…The fat body is a diffuse organ lining the body cavity of insects and consists of two types of cells: trophocytes of mesodermic origin and oenocytes of ectodermic origin. Trophocytes are thought to play a local role in intermediary metabolism by storing lipids, carbohydrates and proteins (Dean et al 1985, Martins & Pimenta 2008. Larval oenocytes were first described in insects over 140 years ago.…”

mentioning

confidence: 99%

“…Larval oenocytes were first described in insects over 140 years ago. Since then, their function has remained unclear and they have been investigated in largely descriptive studies implicating larval oenocytes in processes such as cuticle synthesis and the regulation of hemolymph composition (reviewed by Dean et al 1985). Gutierrez et al (2007) demonstrated that oenocytes from Drosophila melanogaster larva store lipid as cytoplasm droplets and appear to play a crucial role in insect development.…”

mentioning

confidence: 99%

Insights into the transcriptome of oenocytes from Aedes aegypti pupae

Martins

Ramalho-Ortigão

Lobo

et al. 2011

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Rabossi

Stoka

Puizdar

et al. 2004

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

During larva to adult transition, the larval fat body of the Medfly (Ceratitis capitata) progressively disintegrates to be replaced by the adult one, after imago ecdysis. Here we show that a temporal correlation exists among the microscopy images of fat body progressive disintegration, the activation of fat body lysosomes (as judged by acid phosphatase activity), and the activity of a novel fat body aspartyl proteinase. The enzyme was purified and partially characterized. This proteinase exhibited a wide range of acid isoforms with isoelectric points from 5.6 to 7.3, an optimum pH of 3.0 for hemoglobin digestion, and was completely inhibited by pepstatin A. The apparent molecular weight was estimated (42 +/- 1 kDa) and the protein was characterized as N-glycosylated, judging from affinity to Concanavalin A. From the biochemical characteristics, the enzyme that we called "Early Metamorphosis Aspartyl Proteinase" (EMAP) appears to be similar to mammalian Cathepsin D. However, the N-terminal sequence of EMAP showed no similarity with any known animal Cathepsins and exhibited an important instability to neutral and alkaline pH. This feature seems to be a peculiar characteristic of insect aspartyl proteinases. The temporal activity profile of EMAP during metamorphosis correlated well with the microscopy images of fat body cell autolytic death. Our data support the notion that EMAP is a metamorphosis-specific lysosomal proteinase, mostly expressed during larval fat body histolysis.

show abstract

Structure of the Fat Body

Cited by 73 publications

References 151 publications

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Insights into the transcriptome of oenocytes from Aedes aegypti pupae

Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Contact Info

Product

Resources

About