Structure determination of blood group type glycolipids of cat small intestine by mass fragmentography

Breimer, Michael E.; Hansson, Gunnar C.; Karlsson, Karl‐Anders; Leffler, Hakon; Pimlott, Weston; Samuelsson, Bo E.

doi:10.1016/0014-5793(78)80518-3

Cited by 27 publications

(3 citation statements)

References 9 publications

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“…The carbohydrate chains of glycosphingolipids are extremely variable, and are known to be involved in self-not self recognition as blood group antigens and receptors for bacterial toxins [ 11 ]. Furthermore, the glycolipid pattern is species-specific [11][12][13] and differs between epithelial and non-epithelial tissue [14]. Carbohydrates at the cell surface have been implicated as possible receptors for attaching bacteria [5,15,16] but no epithelial cell component interacting with bacteria has been identified.…”

Section: Introductionmentioning

confidence: 99%

Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes

Leffler

1980

FEMS Microbiology Letters

178

273

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Section: Introductionmentioning

confidence: 99%

Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes

Leffler

1980

FEMS Microbiology Letters

178

273

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“…4, 6 , 8, 10, 11) and as mass spectra at some points of evaporation (Figs. 5,7,9,10). Finally, the concluded compounds are indicated in the thin-layer chromatogram ( Fig.…”

Section: Presentation Of Resultsmentioning

confidence: 97%

Selected ion monitoring of glycosphingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit

Breimer

Hansson

Karlsson

et al. 1979

Biol. Mass Spectrom.

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A novel application of selected ion monitoring was used for a mixture of non-acid glycosphingolipids of one rabbit small intestine. Earlier studies of permethylated and permethylated-reduced (LiAIH4) derivatives of model compounds have revealed a specificity and abundance of saccharide ions (terminal monosaccharide(s), disaccharide, trisaccharide, etc., and all sugars plus fatty acid) and of ceramide fragments that permit a conclusive detection of separate glycolipid species in a mixture. The sample (50-200 micrograms) was evaporated slowly (1-5 degrees C min-1 from 150-350 degrees C) from the direct inlet probe of an MS 902 mass spectrometer (electron ionization). Mass spectra with fragments up to about m/z 200 were collected on-line by a computer system. A successive partial separation was obtained for glycolipids with from one up to seven sugars. The structures of eight different compounds were identified. They all had 16:0, 22:0 and 24:0 2-hydroxy fatty acids and 18:0 trihydroxy base (phytosphingosine) as major ceramide components. The dominating complex glycolipid was a hexaglycosylceramide with a blood group B type of sequence. A blood group A type sequence was found in a second hexaglycosylceramide. In support of this, the native mixture showed blood group A and B activity. An intense peak, m/z 182, collected from methylated derivatives were evidence for a dominating type 2 carbohydrate chain of the core tetrasaccharide.

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“…This is not possible with conventional degradation methods which are unable to give reliable data when 25 sugars are present [ 1 S -20]. Of particular interest is our recent application of the technique on mixtures of glycolipid antigens [10,21]. By a temperature-programmed distillation in the ion source and a continuous recording of spectra, a specific info~ation from separate sequences may now be obtained from mixtures with l-l 2 sugars.…”

Section: Resultsmentioning

confidence: 99%

Sequencing of oligosaccharides by mass spectrometry applied on a 12‐sugar glycolipid

Breimer¹,

Hansson²,

Karlsson³

et al. 1981

FEBS Letters

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Structure determination of blood group type glycolipids of cat small intestine by mass fragmentography

Cited by 27 publications

References 9 publications

Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes

Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes

Selected ion monitoring of glycosphingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit

Sequencing of oligosaccharides by mass spectrometry applied on a 12‐sugar glycolipid

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