Structure and properties of silk fibroin–poly(vinyl alcohol) gel

Li, Mingzhong; Lu, Shenzhou; Wu, Zhengyu; Tan, Ke Tian; Minoura, Norihiko; Kuga, Shigenori

doi:10.1016/s0141-8130(02)00007-7

Cited by 111 publications

(81 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The process of preparing SF aqueous solution is described elsewhere [11,21]. To be specific, cocoons of A. pernyi were boiled for 30 min in a 0.25 wt% Na 2 CO 3 aqueous solution to remove sericin.…”

Section: Preparation Of Sf Aqueous Solutionsmentioning

confidence: 99%

“…Secondary conformation of hydrogels from pure A. pernyi SF, pure B. mori SF, and SFs blended with PEG are shown in the XRD curves in Figure 3. For the diffraction peaks of silk fibroin, please see [21,24,25]. In Figure 3(a), the diffraction peaks of pure A. pernyi SF hydrogels, shown in curve (A), appear around 16.5 • , 20.2 • , 24.9 • , and 30.9 • and are attributed to β-sheet structure.…”

Section: X-ray Diffractionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

Self Cite

View full text Add to dashboard Cite

Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of the Antheraea pernyi (A. pernyi) SF solution was significantly shorter than that of the Bombyx mori (B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins of A. pernyi and B. mori. The gelation time of both A. pernyi SF and B. mori SF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation of β-sheets.

show abstract

Section: Preparation Of Sf Aqueous Solutionsmentioning

confidence: 99%

Section: X-ray Diffractionmentioning

confidence: 99%

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cocoons were degummed three times by soaking in Na 2 CO 3 1 g/L solution at 85 • C for 30 min. Afterward, the obtained SF fibers were rinsed with distilled water to remove Na 2 CO 3 residues and dried at room temperature (25 • C) for 24 h. A ternary solvent of CaCl 2 :ethanol:H 2 O in a molar ratio of 1:2:8 was used to dissolve the SF fibers to 10% (w/v) at 85 • C for 1.5 h. The SF salt solution was dialyzed by a cellulose membrane (MWCO 3500, Viscofan®, Spain) against distilled water for 72 h at 4 • C, with changes every 24 h [5,14]. The obtained concentration of SF solution was 5% (w/v), which was determined by casting the solution in a petri dish and weighing the dried mass after solvent evaporation.…”

Section: Solutions Preparationmentioning

confidence: 99%

“…It has been used in diverse applications, such as hydrogels synthesis, supports for enzymes immobilization, drug delivery, and etc. [3,5,6]. Konjac glucomannan (KGM) is also a biodegradable and biocompatible material [1].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Properties of Silk Fibroin/Konjac Glucomannan Blend Beads

et al. 2018

View full text Add to dashboard Cite

Silk fibroin (SF) and konjac glucomannan (KGM) are promising materials in the biomedical field due to their low toxicity, biocompatibility, biodegradability and low immune response. Beads of these natural polymers are interesting scaffolds for biomedical applications, but their fabrication is a challenge due to their low stability and the necessary adaptation of their chemical and mechanical properties to be successfully applied. In that sense, this study aimed to synthesize a blend of silk fibroin and konjac glucomannan (SF/KGM) in the form of porous beads obtained through dripping into liquid nitrogen, with a post-treatment using ethanol. Intermolecular hydrogen bonds promoted the integration of SF and KGM. Treated beads showed higher porous size, crystallinity, and stability than untreated beads. Characterization analyses by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric (TGA), and X-ray diffraction (XDR) evidenced that ethanol treatment allows a conformational transition from silk I to silk II in SF and an increase in the KGM deacetylation. Those chemical changes significantly enhanced the mechanical resistance of SF/KGM beads in comparison to pure SF and KGM beads. Moreover, samples showed cytocompatibility with HaCaT and BALB/c 3T3 cells.

show abstract

“…Many international scientific studies confirm the suitability of natural silk material in surgery and medicine [7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Implantsmentioning

confidence: 99%

Vascular Implants Reinforced with Natural Silk Yarns

Kancevicha

Lukyanchikovs

2014

Materials Science. Textile and Clothing Technology

View full text Add to dashboard Cite

Abstract. In the course of this research work woven synthetic prosthesis reinforced with natural silk yarns was designed and manufactured. Biomechanical properties of surgical natural silk were studied during designing of the structure of the walls of the prosthesis. The experiment gave good data of elasticity and strain properties in a process of laboratory testing of samples of natural fibers. The previous practical experience was also used, for example, various vascular prostheses made from polyurethane complex yarns and polyester monofilaments. As a result, the authors of the scientific work proposed a combined wall structure of vascular prosthesis, consisting of polyester, polyurethane, and natural silk surgical yarns. After the implantation of the synthetic prosthesis reinforced with natural silk yarns, these natural silk yarns completely dissolve in the body for several years. Living tissue is gradually growing into the walls of synthetic prosthesis, replacing dissolved natural silk yarns. Experimental tests confirmed that a modulus of elasticity of innovative prosthesis is close to modulus of elasticity of natural blood vessels. The innovative structure of blood vessel prosthesis combined with such parameters can provide a long-term normal hemodynamics.

show abstract

Structure and properties of silk fibroin–poly(vinyl alcohol) gel

Cited by 111 publications

References 24 publications

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Synthesis and Properties of Silk Fibroin/Konjac Glucomannan Blend Beads

Vascular Implants Reinforced with Natural Silk Yarns

Contact Info

Product

Resources

About