Structure and function of hepatic stellate cells

Senoo, Haruki

doi:10.1007/s00795-003-0230-3

Cited by 183 publications

(141 citation statements)

References 68 publications

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…Normal, quiescent HSCs store over 80% of total body vitamin A and maintain vitamin A blood homoeostasis (Sato et al 2003). HSCs are located between parenchymal cell plates and sinusoidal endothelial cells and extend welldeveloped, long processes surrounding sinusoids in vivo as pericytes (Senoo 2004). They are the predominant cell type producing ECM components as well as ECM-degrading metalloproteases, indicating that they play a pivotal role in ECM remodelling in both normal and pathological liver conditions (Kmiec 2001).…”

Section: Introductionmentioning

confidence: 99%

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

Krause

Saghatolislam

Koenig

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Primary hepatocytes lose their differentiated functions rapidly when in culture. Our aim was to maintain the differentiated status of hepatocytes in vitro by means of vital hepatic stellate cells (HSCs), their soluble and particulate factors and lipid extracts. Hepatocytes were placed into collagen-coated culture dishes in the presence of HSCs at different ages of pre-culture, with or without direct cell to cell contacts, at different cell ratios and in monoculture with cellular HSC components in place of vital cells. Changes in morphology and enhancement of phosphoenolpyruvate carboxykinase (PCK) activity by glucagon were used to determine the differentiated status of hepatocytes in 2d-short-term culture. HSCs proved able to maintain the differentiated function of hepatocytes in coculture either by direct cell contacts or via factors derived from HSC-conditioned medium. In comparison, however, without cellular contact to hepatocytes five to ten times as many HSCs were necessary to increase the PCK activity to the same degree as in the presence of intercellular contacts. Whereas stimulation in the presence of HSC/hepatocyte contacts was independent of HSC culture age only quiescent, resting HSCs (precultured for 1-2 d) were able to stimulate hepatocytes significantly via soluble factors. Culturing of hepatocytes with a lipid extract or a particulate fraction from HSCs clearly displayed a very strong beneficial effect on enzyme activity and morphology. HSCs maintain hepatocyte function and structure through preferentially cell-bound signalling and transfer of lipids.

show abstract

Section: Introductionmentioning

confidence: 99%

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

Krause

Saghatolislam

Koenig

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

“…For all of these diseases, there is a common pathologic mechanism that leads to fibrosis: the generation and proliferation of smooth muscle -actin (-SMA)-positive myofibroblasts of periportal and perisinusoidal origin that arise as a consequence of the activation of hepatic stellate cells (HSCs). HSCs exist in the normal liver as quiescent retinoid-storing cells, which in response to injury activate to become proliferative, profibrogenic cells [1,2]. This event can be recapitulated in a culture model in which isolated HSCs are cultured on plastic in serumcontaining media.…”

Section: Introductionmentioning

confidence: 99%

Zinc supplementation attenuates ethanol- and acetaldehyde-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS) production and by influencing intracellular signaling

Szuster‐Ciesielska

Plewka

Daniluk

et al. 2009

Biochemical Pharmacology

View full text Add to dashboard Cite

To cite this version:Agnieszka Szuster-Ciesielska, Krzysztof Plewka, Jadwiga Daniluk, Martyna Kandefer-Szerszeń. Zinc supplementation attenuates ethanol-and acetaldehyde-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS) production and by influencing intracellular signaling. Biochemical Pharmacology, Elsevier, 2009, 78 (3) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

“…Homeostasis of vitamin A is rigidly controlled in the body (Blomhoff et al, 1990) with hepatic stellate cells (vitamin A-storing cells, fat-storing cells, interstitial cells, Ito cells) playing pivotal roles in its regulation (Wake, 1980;Senoo et al, 1984Senoo et al, , 1997Senoo et al, , 1998Blomhoff and Wake, 1991;Senoo and Hata, 1994;Senoo, 2004).…”

mentioning

confidence: 99%

Vitamin A distribution and content in tissues of the lamprey, Lampetra japonica

et al. 2004

Self Cite

View full text Add to dashboard Cite

Vitamin A (retinol and retinyl ester) distribution and content in tissues of a lamprey (Lampetra japonica) were analyzed by morphological methods, namely, gold chloride staining, fluorescence microscopy to detect specific vitamin A autofluorescence, and electron microscopy, as well as high-performance liquid chromatography (HPLC). Hepatic stellate cells showed an abundance of vitamin A stored in lipid droplets in their cytoplasm. Similar cells storing vitamin A were present in the intestine, kidney, gill, and heart in both female and male lampreys. Morphological data obtained by gold chloride staining method, fluorescence microscopy, transmission electron microscopy, and HPLC quantification of retinol were consistent. The highest level of total retinol measured by HPLC was found in the intestine. The second and third highest concentrations of vitamin A were found in the liver and the kidney, respectively. These vitamin A-storing cells were not epithelial cells, but mesoderm-derived cells. We propose as a hypothesis that these cells belong to the stellate cell system (family) that stores vitamin A and regulates homeostasis of the vitamin in the whole body in the lamprey. Fibroblastic cells in the skin and somatic muscle stored little vitamin A. These results indicate that there is difference in the vitamin A-storing capacity between the splanchnic and intermediate mesoderm-derived cells (stellate cells) and somatic and dorsal mesoderm-derived cells (fibroblasts) in the lamprey. Stellate cells derived from the splanchnic and intermediate mesoderm have high capacity and fibroblasts derived from the somatic and dorsal mesoderm have low capacity for the storage of vitamin A in the lamprey.

show abstract

Structure and function of hepatic stellate cells

Cited by 183 publications

References 68 publications

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

Zinc supplementation attenuates ethanol- and acetaldehyde-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS) production and by influencing intracellular signaling

Vitamin A distribution and content in tissues of the lamprey, Lampetra japonica

Contact Info

Product

Resources

About