Structure analysis of the spinneret from Bombyx mori and its influence on silk qualities

Guo, Nangkuo; Lu, Kunpeng; Cheng, Longdi; Li, Zhi; Wu, Chunman; Liu, Zulan; Liang, Shubo; Chen, Sihao; Chen, Wenhao; Jiang, Chenlong; Dai, Fangyin

doi:10.1016/j.ijbiomac.2018.12.219

Cited by 11 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fine silk has higher economic value in sericulture, but the genetic basis of fiber fineness is unknown. We previously found that a part of the spinneret, the silk press, is related to fineness 43 . Here we performed RNA-seq of the silk press in four strains including two fine silk strains (Suxiu, Chunfeng) and two coarse silk strains (Xiafang, Qiubai) (Fig.…”

Section: Resultsmentioning

confidence: 99%

High-resolution silkworm pan-genome provides genetic insights into artificial selection and ecological adaptation

Tong

Han

et al. 2022

Nat Commun

Self Cite

View full text Add to dashboard Cite

The silkworm Bombyx mori is an important economic insect for producing silk, the “queen of fabrics”. The currently available genomes limit the understanding of its genetic diversity and the discovery of valuable alleles for breeding. Here, we deeply re-sequence 1,078 silkworms and assemble long-read genomes for 545 representatives. We construct a high-resolution pan-genome dataset representing almost the entire genomic content in the silkworm. We find that the silkworm population harbors a high density of genomic variants and identify 7308 new genes, 4260 (22%) core genes, and 3,432,266 non-redundant structure variations (SVs). We reveal hundreds of genes and SVs that may contribute to the artificial selection (domestication and breeding) of silkworm. Further, we focus on four genes responsible, respectively, for two economic (silk yield and silk fineness) and two ecologically adaptive traits (egg diapause and aposematic coloration). Taken together, our population-scale genomic resources will promote functional genomics studies and breeding improvement for silkworm.

show abstract

Section: Resultsmentioning

confidence: 99%

High-resolution silkworm pan-genome provides genetic insights into artificial selection and ecological adaptation

Tong

Han

et al. 2022

Nat Commun

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fine silk has higher economic value in sericulture but the genetic basis of fiber fineness is unknown. We previously found that a part of the spinneret, the silk press, is related to fineness through comparisons between fine silk (SX, CF) and coarse silk (XF, QB) strains 29 (Fig. 6b, Extended Data Fig.…”

Section: Genetic Basis Of Breedingmentioning

confidence: 97%

Reference genomes of 545 silkworms enable high-throughput exploring genotype-phenotype relationships

Tong

Han

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The silkworm Bombyx mori is a domestic insect for silk production and a lepidopteran model. The currently available genomes limit a full understanding of its genetic and phenotypic diversity. Here we assembled long-read genomes of 545 domestic and wild silkworms and constructed a high-resolution pan-genome dataset. We found that the silkworm population harbors extremely variable genomes containing 7,308 new gene families, 4,260 (22%) core gene families, and 3,432,266 non-redundant SVs. We deciphered a series of causal genes and variants associated with domestication, breeding, and ecological adaptation traits, and experimentally validated two of those genes using CRISPR-Cas9 or RNA interference. This unprecedented large-scale genomic resource allows for high-throughput screening of interesting traits for functional genomic research and breeding improvement of silkworms and may serve as a guideline for traits decoding in other species.

show abstract

“…For example, when cocooning, Bombyx mori adheres freshly secreted silkworm (FS-S) silk onto a base surface (the inner surface of the cocoon) and then swings its head while utilizing the adhesion between the silk fiber and base surface to continuously draw the fiber out of its spinneret. This process is repeated until cocooning is completed. − The head swinging maintains the spinneret close to the base surface and ensures that the FS-S silk fiber is firmly adhered . Thus, an angle exists between the spinneret and FS-S silk fiber on the base surfaceFS-S silk will bend to a certain extent .…”

Section: Introductionmentioning

confidence: 99%

“…31−33 The head swinging maintains the spinneret close to the base surface and ensures that the FS-S silk fiber is firmly adhered. 34 Thus, an angle exists between the spinneret and FS-S silk fiber on the base surface�FS-S silk will bend to a certain extent. 19 Similarly, spider FS-MA silk may also bend as it gushes out of the spinneret.…”

Section: ■ Introductionmentioning

confidence: 99%

Bending–Spinning Produces Silkworm and Spider Silk with Enhanced Mechanical Properties

Peng

Zhao

et al. 2023

Macromolecules

View full text Add to dashboard Cite

Natural silk-spinning behavior endows freshly secreted (FS) silk with a bending angle. This phenomenon is universal among silkworms and spiders but remains unexplained. In this study, the angle of freshly secreted silkworm (FS-S) silk was revealed to be 70.23°–87.93° and that of freshly secreted spider major ampullate (FS-MA) silk to be 0°–50°. Furthermore, the mechanical properties of FS-S silk and FS-MA silk changed after bending, first increasing and then decreasing as the bending angle was increased (0°–360°). The angle corresponding to FS-S silk with the best mechanical properties is 67.5°–90°, and for FS-MA silk, it is 45°. Structural analysis showed that the β-sheet, crystallinity, and crystal size increased with bending angle and the orientation decreased with bending angle. Regular change of microstructure explains the trend for the mechanical property change with bending angle. These findings suggest that bending–spinning produces silkworm and spider silk with enhanced mechanical properties.

show abstract

Structure analysis of the spinneret from Bombyx mori and its influence on silk qualities

Cited by 11 publications

References 15 publications

High-resolution silkworm pan-genome provides genetic insights into artificial selection and ecological adaptation

High-resolution silkworm pan-genome provides genetic insights into artificial selection and ecological adaptation

Reference genomes of 545 silkworms enable high-throughput exploring genotype-phenotype relationships

Bending–Spinning Produces Silkworm and Spider Silk with Enhanced Mechanical Properties

Contact Info

Product

Resources

About