Structural Tightening and Interdomain Communication in the Catalytic Cycle of Phosphoglycerate Kinase

Marston, J.P.M.; Cliff, Matthew J.; Reed, Michelle A.C.; Blackburn, G. Michael; Hounslow, Andrea M.; Craven, C. Jeremy; Waltho, Jonathan P.

doi:10.1016/j.jmb.2009.11.052

Cited by 7 publications

(8 citation statements)

References 52 publications

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“…We were able to identify three of these resonances as T yr-380, Phe-342 and His-62 based on assignments previousl y described in the literature (Fairbrother et al 1989a (Fairbrother et al 1989a), where, in the absence of ADP and MgCl 2 which is essential for yeast PGK catal ysis , no chemical shift changes were observed for these resonances. That we observed changes to the chemical shift of residues in both the N-and C-termini as a result of including ADP, is likel y an example of interdomain cooperativit y and communication and has been observed in G. stearothermophilus PGK (Marston et al 2010). The ∆δH for T yr-380, Phe-342 and His-62 was used to fit Equation 10 ( Fig.…”

Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 64%

“…Marston et al (Marston et al 2010) for G. stearothermophilus PGK. In this model the active site of PGK has two conformations in equilibrium (E), one with a weak affinit y for ligands (A) and one with a strong affinit y for ligands (B).…”

Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 93%

“…This difference in binding affinit y is reflected b y a change to the dissociation curve (Figure 6d and 6e) and curves consistent with the expected trend of this model were observed in the ∆δH of His-62 and T yr-380 as the TSA is formed upon titration of ADP to form the TSA (Figure 6a and 6b). Although a dissociation constant could not be calculated due to the tight binding, a twostate cooperative binding model suggests K D 3 P G ( F ) is significantl y smaller than K D 3 P G (Table 2), and K D values for TSA complexes have been estimated to be as low 10 -1 4 M (Marston et al 2010). However, the average K…”

Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 98%

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MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase

McCormick

Forget

Syvitski

et al. 2017

Biochem. Cell Biol.

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The phospho-transfer mechanism of yeast phosphoglycerate kinase (PGK) has been probed through formation of trifluoromagnesate (MgF) and tetrafluoroaluminate (AlF) transition state analogue complexes and analyzed using F,H waterLOGSY and H chemical shift perturbation NMR spectroscopy. We observed the firstF NMR spectroscopic evidence for the formation of metal fluoride transition state analogues of yeast PGK and also observed significant changes to proton chemical shifts of PGK in the presence, but not in the absence, of fluoride upon titration of ligands, providing indirect evidence of the formation of a closed ternary transition state. WaterLOGSY NMR spectroscopy experiments using an uncompetitive model were used in an attempt to measure ligand binding affinities within the transition state analogue complexes.

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Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 64%

Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 93%

Section: Results and Discussion 1 9 F Nmr Spectroscopy Of Yeast Pgk•3mentioning

confidence: 98%

See 1 more Smart Citation

MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase

McCormick

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Syvitski

et al. 2017

Biochem. Cell Biol.

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“…It is clear that the overall amino acid composition and the reduced number of weak interactions tend to destabilize the enzyme but most probably, more specific modifications may play a role in the bending motion. To address this issue, we first focused our attention on the three helices shown to form the inter-domain region and the surrounding regions (Marston et al 2010;Inoue et al 2010;Auerbach et al 1997). These three helices (helices 5, 12 and 13 in PsyPGK) should be directly involved if an adaptive bending motion occurred in the psychrophilic enzyme.…”

Section: Increased Flexibility Around the Hinge Regionmentioning

confidence: 99%

“…Each domain plays a distinct role in substrate recognition; the N-terminal domain binds 1,3-BPG using a basic patch (Harlos et al 1992) and Mg-ATP is bound to the C-terminal domain (Davies et al 1993). These enzymes operate via a hinge-bending motion of the two domains which brings the substrates at spatial proximity to facilitate phosphate transfer (Bernstein and Hol 1997;Marston et al 2010;Inoue et al 2010;Cliff et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase

et al. 2019

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Crystal structures of phosphoglycerate kinase (PGK) from the psychrophile Pseudomonas sp. TACII 18 have been determined at high resolution by X-ray crystallography methods and compared with mesophilic, thermophilic and hyperthermophilic counterparts. PGK is a two-domain enzyme undergoing large domain movements to catalyze the production of ATP from 1,3-biphosphoglycerate and ADP. Whereas the conformational dynamics sustaining the catalytic mechanism of this hingebending enzyme now seems rather clear, the determinants which underlie high catalytic efficiency at low temperatures of this psychrophilic PGK were unknown. The comparison of the three-dimensional structures shows that multiple (global and local) specific adaptations have been brought about by this enzyme. Together, these reside in an overall increased flexibility of the cold-adapted PGK thereby allowing a better accessibility to the active site, but also a potentially more disordered transition state of the psychrophilic enzyme, due to the destabilization of some catalytic residues.

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Effects of exogenous spermidine on photosynthetic capacity and expression of Calvin cycle genes in salt-stressed cucumber seedlings

Shu

Chen

et al. 2014

J Plant Res

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We investigated the effects of exogenous spermidine (Spd) on growth, photosynthesis and expression of the Calvin cycle-related genes in cucumber seedlings (Cucumis sativus L.) exposed to NaCl stress. Salt stress reduced net photosynthetic rates (PN), actual photochemical efficiency of PSII (ΦPSII) and inhibited plant growth. Application of exogenous Spd to salinized nutrient solution alleviated salinity-induced the inhibition of plant growth, together with an increase in PN and ΦPSII. Salinity markedly reduced the maximum carboxylase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Vcmax), the maximal velocity of RuBP regeneration (Jmax), triose-phosphate utilization capacity (TPU) and carboxylation efficiency (CE). Spd alleviated the negative effects on CO2 assimilation induced by salt stress. Moreover, Spd significantly increased the activities and contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and fructose-1,6-biphosphate aldolase (ALD; aldolase) in the salt-stressed cucumber leaves. On the other hand, salinity up-regulated the transcriptional levels of ribulose-1,5-bisphosphate (RCA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribrokinase (PRK) and down-regulated the transcriptional levels of ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RbcL), ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS), ALD, triose-3-phosphate isomerase (TPI), fructose-1,6-bisphosphate phosphatase (FBPase) and 3-phosphoglyceric acid kinase (PGK). However, Spd application to salt-stressed plant roots counteracted salinity-induced mRNA expression changes in most of the above-mentioned genes. These results suggest that Spd could improve photosynthetic capacity through regulating gene expression and activity of key enzymes for CO2 fixation, thus confers tolerance to salinity on cucumber plants.

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Structural Tightening and Interdomain Communication in the Catalytic Cycle of Phosphoglycerate Kinase

Cited by 7 publications

References 52 publications

MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase

MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase

Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase

Effects of exogenous spermidine on photosynthetic capacity and expression of Calvin cycle genes in salt-stressed cucumber seedlings

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Structural Tightening and Interdomain Communication in the Catalytic Cycle of Phosphoglycerate Kinase

Cited by 7 publications

References 52 publications

MgF3−and AlF4−transition state analogue complexes of yeast phosphoglycerate kinase

MgF3−and AlF4−transition state analogue complexes of yeast phosphoglycerate kinase

Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase

Effects of exogenous spermidine on photosynthetic capacity and expression of Calvin cycle genes in salt-stressed cucumber seedlings

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Product

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MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase

MgF₃⁻and AlF₄⁻transition state analogue complexes of yeast phosphoglycerate kinase