Structural Studies of an extracellular polysaccharide, S-657, elaborated by Xanthomonas ATCC 53159

Chowdhury, Tofail A.; Lindberg, Bengt; Lindquist, Ulf; Baird, J. Kevin

doi:10.1016/0008-6215(87)80124-6

Cited by 39 publications

(15 citation statements)

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“…Although the primary structure of S-198 gum [60] is similar to that of gellan [43] [44], welan [55], rhamsan [56], S-657 [57] and S-88 [59] gum, its rheological characteristics differ from gellan (deacylated) [5], native gellan [6] and deacetylated rhamsan [12], but are essentially in agreement with those of welan [51], native rhamsan [52] [53], S-657 [54] and S-88 [58] gums. Thus, S-198 gum molecules may involve intramolecular associations, as do welan, rhamsan, S-657 and S-88 gum molecules.…”

Section: Discussionmentioning

confidence: 89%

“…Intramolecular associations of rhamsan gum in aqueous solution. [56], and substitution at O-3 of every D-glucosyl residue next to the D-glucuronosyl residue by α-L-rhamnosyl disaccharide side-chains for S-657 gum [57]. The methyl groups of L-rhamnosyl residues played a dominant role in the thermal stability due to the formation of intramolecular van der Waals forces of attraction.…”

Section: Introductionmentioning

confidence: 99%

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Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

Tako¹,

Kitajima²,

Yogi³

et al. 2016

ABC

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The structure-function relationship of a gellan family of polysaccharides, S-198 gum produced by Alcaligenes ATCC31853 was investigated in terms of rheological aspects. The flow curves of S-198 gum showed plastic behavior above 0.3%. Gelation did not occur in S-198 gum solution at low temperature (0˚C), even at 0.8%. Both the viscosity and the elastic modulus remained constant with increasing temperature up to 80˚C. The elastic modulus decreased a little with the addition of CaCl2 (6.8 mM), but then once again remained constant up to 80˚C. The highest elastic modulus was observed for deacylated gellan gum with the addition of CaCl 2 and increased slightly with increasing temperature up to 80˚C, which was considered to be a transition temperature, after which it decreased rapidly. The elastic modulus of S-198 gum in the presence of urea (4.0 M) was lower than that in aqueous solution at low temperature (0˚C), but remained constant with increasing temperature up to 80˚C. The intramolecular associations, (hydrogen bonding and van der Waals forces of attraction), of S-198 gum molecules in aqueous solutions were proposed. The gellan family of polysaccharides, S-198, S-88, S-657, rhamsan, welan and gellan gum, provided a good opportunity to investigate the structure-function relationship for polysaccharides.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

Tako¹,

Kitajima²,

Yogi³

et al. 2016

ABC

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“…We have also proposed a model of intramolecular associations of welan 25 and S-657 26 gum, the structure of which is similar to that of gellan, but substituted at O-3 of every D-glucosyl residue next to the L-rhamnosyl residue by either -L-rhamnosyl or -L-mannosyl side chain in the ratio of 2:1 for the former, 27 and substituted at O-3 of every D-glucosyl residue next to the L-rhamnosyl residue by -L-rhamnosyl disaccharide side chains for the latter 28 in aqueous solution, as illustrated in Scheme 2 and Scheme 3. The intramolecular associations take place between the OH-4 of the D-glucosyl residue and the adjacent hemiacetal oxygen atom of the L-rhamnosyl residue with hydrogen bonding as in gellan gum and between the methyl group of the L-rhamnosyl residue and the adjacent hemiacetal oxygen atom of the D-glucosyl residue with van der Waals interaction.…”

Section: Yymentioning

confidence: 94%

Molecular Origin for the Thermal Stability of S-88 Gum Produced by Pseudomonas ATCC 31554

Tako

Tamaki

2005

Polym J

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ABSTRACT:Non-Newtonian behavior and dynamic viscoelasticity of S-88 gum produced by Pseudomonas ATCC 31554 in aqueous solution were measured with a rheogoniometer. The S-88 gum showed shear-thinning behavior at a concentration of 0.1%, but plastic behavior above 0.3%. Gelation did not occur even at 1.0% and low temperature (0 C). The viscosity decreased gradually with increasing temperature at concentrations below 0.3%, but increased above 0.8%, where a transition temperature was observed at 45 C. The elastic modulus showed a constant value during increasing temperature at concentrations above 0.5%. Gelation did not occur in the presence of CaCl 2 (6.8 mM) even at a low (0 C) temperature. A little decrease of the elastic modulus was observed in urea (4.0 M), 0.05 M NaOH, and 85% DMSO solution and kept large values during increasing temperature. The thermal stability for viscosity and dynamic viscoelasticity of S-88 gum molecules might be attributed to intramolecular associations, possible mode of which was proposed.

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“…This exopolysaccharide has been known to be produced by S. paucimobilis strain P4 (DSM 6418) (Lobas et al, 1994), a strain that was isolated by a selective culturing method (Lobas et al, 1992) from a culture of [P.] paucimobilis NCIB 11942 (Anson et al, 1987). Also of particular interest is the fact that PS-EDIV does not contain -mannose, which has been found as a constituent of sphingans S-130, S-88, S-198 and NW-11 (Jansson et al, 1985(Jansson et al, , 1986Chowdhury et al, 1987a ;Robison & Stipanovic, 1989). In addition, there is some similarity between exopolysaccharides produced by [P.] paucimobilis RMPD strains 17a and 17b (Hebbar et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Fredrickson et al, 1991Fredrickson et al, , 1995Harms et al, 1995 ;Karlson et al, 1995 ;Mueller et al, 1990 ;Nohynek et al, 1996a, b ;Wittich E. B. M. Denner and others et al, 1992 ;Zipper et al, 1996), as bacterial antagonists of phytopathogenic fungi (Berg & Balin, 1994) and for the production of industrially useful exopolysaccharides (Moorehouse, 1987 ;Chandrasekaran & Radha, 1995). Certain structurally related exopolysaccharides referred to as ' sphingans ' (Pollock, 1993) are synthesized by several Sphingomonas strains, and include the following : heteropolysaccharide S-7 (Kang & McNeely, 1976), gellan (S-60), S-88, welan (S-130) (Kang & Veeder, 1982a, b, 1985Kang et al, 1982 ;O'Neill et al, 1983), rhamsan (S-194) (Peik et al, 1983), S-198 (Peik et al, 1985), S-657 (Chowdhury et al, 1987b), NW11 (Robison & Stipanovic, 1989), PS-P4 (Lobas et al, 1994), GS-1 (Ashtaputre & Shah, 1995), I-886 (Falk et al, 1996) and HWR1 (Hashimoto & Murato, 1998). Sphingans share the same carbohydrate-backbone structure (-X-glucose-glucuronic acid-glucose-X-, where X is either -rhamnose or -mannose), to which distinct side-groups are attached, influencing their rheological properties.…”

Section: Abbreviationsmentioning

confidence: 99%

Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan.

Denner

Paukner²,

Kämpfer³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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Strain EDIVT , an exopolysaccharide-producing bacterium, was subjected to polyphasic characterization. The bacterium produced copious amounts of an extracellular polysaccharide, forming slimy, viscous, intensely yellowpigmented colonies on Czapek-Dox (CZD) agar. The culture fluids of the liquid version of CZD medium were highly viscous after cultivation for 5 d. Cells of strain EDIV T were Gram-negative, catalase-positive, oxidase-negative, nonspore-forming, rod-shaped and motile. Comparisons of 16S rDNA gene sequences demonstrated that EDIV T clusters phylogenetically with the species of the genus Sphingomonas sensu stricto. The GMC content of the DNA (645 mol %), the presence of ubiquinone Q-10, the presence of 2-hydroxymyristic acid (14 :0 2-OH) as the major hydroxylated fatty acid, the absence of 3-hydroxy fatty acids and the detection of sym-homospermidine as the major component in the polyamine pattern, together with the presence of sphingoglycolipid, supported this delineation. 16S rDNA sequence analysis indicated that strain EDIV T is most closely related (994 % similarity) to Sphingomonas trueperi LMG 2142 T . DNA-DNA hybridization showed that the level of relatedness to S. trueperi is only 455 %. Further differences were apparent in the cellular fatty acid profile, the polar lipid pattern, the Fouriertransform infrared spectrum and whole-cell proteins and in a number of biochemical characteristics. On the basis of the estimated phylogenetic position derived from 16S rDNA sequence data, DNA-DNA reassociation and phenotypic differences, strain EDIV T (l CIP 106154 T l DSM 13101 T ) was recognized as a new species of Sphingomonas, for which the name Sphingomonas pituitosa sp. nov. is proposed. A component analysis of the exopolysaccharide (named PS-EDIV) suggested that it represents a novel type of sphingan composed of glucose, rhamnose and an unidentified sugar. Glucuronic acid, which is commonly found in sphingans, was absent. The mean molecular mass of PS-EDIV was approximately 3i10 6 Da.Keywords : Sphingomonas pituitosa sp. nov., polyphasic characterization, exopolysaccharide, sphingan INTRODUCTION Interest in the genus Abbreviations :FT-IR, Fourier-transform infrared ; PHB, poly-β-hydroxybutyrate.The EMBL accession number for the 16S rDNA sequence of Sphingomonas pituitosa strain EDIV T is AJ243751.increasingly intense, as there have been several recent reports on the broad catabolic capabilities of strains of Sphingomonas species. These organisms have great potential for biotechnological applications in the degradation, bioremediation and wastewater treatment of xenobiotic pollutants (e.g. Fredrickson et al., 1991Fredrickson et al., , 1995 Harms et al., 1995 ; Karlson et al., 1995 ;Mueller et al., 1990 ; Nohynek et al., 1996a, b E. B. M. Denner and others et al., 1992 ; Zipper et al., 1996), as bacterial antagonists of phytopathogenic fungi (Berg & Balin, 1994) and for the production of industrially useful exopolysaccharides (Moorehouse, 1987 ;Chandrasekaran & Radha, 1995). Certain structura...

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Structural Studies of an extracellular polysaccharide, S-657, elaborated by Xanthomonas ATCC 53159

Cited by 39 publications

References 6 publications

Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

Molecular Origin for the Thermal Stability of S-88 Gum Produced by Pseudomonas ATCC 31554

Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan.

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