Structural requirements for steroid binding and quenching of albumin fluorescence in bovine plasma albumin

Romeu, A; Martino, Elba E.; Stoppani, A. O. M.

doi:10.1016/0005-2795(76)90174-4

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Cited by 15 publications

References 48 publications

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New approach to regioselective synthesis: Using proteins in vitro as guidance—regioselective reduction of steroids with guidance from bovine serum albumin

Liu

Kayser²

2003

Biotech & Bioengineering

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A novel approach for regioselective synthesis is described. The approach uses the unique binding properties of protein as a guide to achieve regioselectivity. Specifically, steroids, e.g., 4-androstene-3,17-dione, were reduced selectively at carbon 17 using bovine serum albumin (BSA) as a regio auxiliary. The approach has resulted in a high yield (ca. 91%) in an environmental friendly aqueous medium. Both (1)H NMR and (13)C NMR data show that the BSA-assisted reduction of the steroid render the reaction regioselective compared to the control reaction. Various control reactions were performed to verify that the unique binding of BSA to steroids was the origin of regioselectivity. After one cycle of the reduction reaction, BSA was reconditioned such that it could be reused five or more times. This novel approach could be used to develop practical and "green" methods for regioselective synthesis.

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New approach to regioselective synthesis: Using proteins in vitro as guidance—regioselective reduction of steroids with guidance from bovine serum albumin

Liu

Kayser²

2003

Biotech & Bioengineering

View full text Add to dashboard Cite

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Bile salts-bovine serum albumin binding: spectroscopic and thermodynamic studies

Picó

Houssier

1989

Biochimica et Biophysica Acta (BBA) - Protein Structure and Mol

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Testosterone-induced proton release and inhibition of tryptic hydrolysis of albumin

Ryan

Baraff

1978

Biochimica et Biophysica Acta (BBA) - General Subjects

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Structural requirements for steroid binding and quenching of albumin fluorescence in bovine plasma albumin

Cited by 15 publications

References 48 publications

New approach to regioselective synthesis: Using proteins in vitro as guidance—regioselective reduction of steroids with guidance from bovine serum albumin

New approach to regioselective synthesis: Using proteins in vitro as guidance—regioselective reduction of steroids with guidance from bovine serum albumin

Bile salts-bovine serum albumin binding: spectroscopic and thermodynamic studies

Testosterone-induced proton release and inhibition of tryptic hydrolysis of albumin

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