Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA

Hubin, Elizabeth A.; Tabib-Salazar, Aline; Humphrey, Laurence J.; Flack, Joshua E; Olinares, Paul Dominic B.; Darst, Seth A.; Campbell, Elizabeth A.; Paget, Mark S. B.

doi:10.1073/pnas.1504942112

Cited by 51 publications

(101 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These include the essential mycobacterial activator RbpA (Paget et al 2001;Tabib-Salazar et al 2013;Hubin et al 2015), GrgA from Chlamydia trachomatis (Bao et al 2012), Crl from E. coli (Pratt and Silhavy 1998), and RsoA from B. subtilis (MacLellan et al 2008). Crl stimulates S -dependent transcription at least in part by interacting with S and promoting its assembly into holoenzyme (Banta et al 2013(Banta et al , 2014.…”

Section: (Ii) Factor Interaction With Transcription Factorsmentioning

confidence: 99%

The essential activities of the bacterial sigma factor

et al. 2017

View full text Add to dashboard Cite

Abstract:Transcription is the first and most heavily regulated step in gene expression. Sigma () factors are general transcription factors that reversibly bind RNA polymerase (RNAP) and mediate transcription of all genes in bacteria. Factors play 3 major roles in the RNA synthesis initiation process: they (i) target RNAP holoenzyme to specific promoters, (ii) melt a region of double-stranded promoter DNA and stabilize it as a single-stranded open complex, and (iii) interact with other DNA-binding transcription factors to contribute complexity to gene expression regulation schemes. Recent structural studies have demonstrated that when factors bind promoter DNA, they capture 1 or more nucleotides that are flipped out of the helical DNA stack and this stabilizes the promoter open-complex intermediate that is required for the initiation of RNA synthesis. This review describes the structure and function of the 70 family of proteins and the essential roles they play in the transcription process.Key words: transcription, bacteria, RNA polymerase, sigma factor, gene expression.

show abstract

Section: (Ii) Factor Interaction With Transcription Factorsmentioning

confidence: 99%

The essential activities of the bacterial sigma factor

et al. 2017

View full text Add to dashboard Cite

show abstract

“…RbpA consists of a central RbpA core domain (RCD) flanked by an unstructured 26-aa N-terminal tail and a C-terminal interaction domain (SID) linked to the RCD by a 15-aa basic linker (BL) (68,71,72). RbpA forms a stable binary complex with the 2-domain of group 1 ( A in M. tuberculosis) and certain group 2 ( B in M. tuberculosis) factors through its SID (68,71,72), with additional contacts made between the N terminus and the factor (68).…”

Section: Factors: the Generals Of Stress Responsesmentioning

confidence: 99%

“…RbpA forms a stable binary complex with the 2-domain of group 1 ( A in M. tuberculosis) and certain group 2 ( B in M. tuberculosis) factors through its SID (68,71,72), with additional contacts made between the N terminus and the factor (68). Based on structural modeling, the RbpA BL domain and adjacent residues interact with the DNA phosphate backbone of the nontemplate strand upstream of the Ϫ10 promoter element in the RP o conformation (68).…”

Section: Factors: the Generals Of Stress Responsesmentioning

confidence: 99%

“…Based on structural modeling, the RbpA BL domain and adjacent residues interact with the DNA phosphate backbone of the nontemplate strand upstream of the Ϫ10 promoter element in the RP o conformation (68). Additional contacts between RbpA and RNAP ␤ have been proposed based on cross-linking experiments (63, 73,74), but the recent structural modeling of RbpA onto an RNAP-promoter open complex would be incompatible with these interactions (71), suggesting that further analysis will be needed to resolve these inconsistencies. RbpA has been shown to increase the affinity of the factor to the core RNAP, increase the affinity of RNAP holoenzyme to promoter DNA, and facilitate the formation of RP o (71,75,76), all of which could contribute to the ability of RbpA to promote RNAP-promoter complex formation and stability.…”

Section: Factors: the Generals Of Stress Responsesmentioning

confidence: 99%

See 1 more Smart Citation

Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks

2016

View full text Add to dashboard Cite

Regulating responses to stress is critical for all bacteria, whether they are environmental, commensal, or pathogenic species. For pathogenic bacteria, successful colonization and survival in the host are dependent on adaptation to diverse conditions imposed by the host tissue architecture and the immune response. Once the bacterium senses a hostile environment, it must enact a change in physiology that contributes to the organism's survival strategy. Inappropriate responses have consequences; hence, the execution of the appropriate response is essential for survival of the bacterium in its niche. Stress responses are most often regulated at the level of gene expression and, more specifically, transcription. This minireview focuses on mechanisms of regulating transcription initiation that are required by Mycobacterium tuberculosis to respond to the arsenal of defenses imposed by the host during infection. In particular, we highlight how certain features of M. tuberculosis physiology allow this pathogen to respond swiftly and effectively to host defenses. By enacting highly integrated and coordinated gene expression changes in response to stress, M. tuberculosis is prepared for battle against the host defense and able to persist within the human population.T he survival of any organism relies on its ability to sense and respond to changes in its environment. For bacteria, stress responses are primarily mediated through the regulation of gene expression. By integrating multiple molecular approaches to gene regulation, pathogenic bacteria are able to orchestrate conditionspecific patterns that promote survival and pathogenesis in the face of a strong immune response. This minireview focuses on mechanisms of transcription regulation required for stress responses in one of the most successful and deadly pathogens in the world, Mycobacterium tuberculosis. M. tuberculosis has coexisted with humans for Ͼ50,000 years (1) and continues to cause more than 1.5 million deaths a year (2). The coevolution of M. tuberculosis with the human host response to infection has resulted in a pathogen that is specialized for long-term infection in people. Tuberculosis is a complex disease that requires the bacteria to multiply within phagocytes, survive extracellularly in hypoxic and necrotic granulomas, and endure a robust immune response to persist in the host. During infection, the host immune response restrains M. tuberculosis from proliferating by imposing a battery of defenses, including reactive oxygen and nitrogen stress, hypoxia, acid stress, genotoxic stress, cell surface stress, and starvation (3). Despite this onslaught of attacks, M. tuberculosis is able to persist for the lifetime of the host, indicating that this pathogen has highly effective molecular mechanisms to resist host-inflicted damage. In order to enact these defenses and facilitate this specialized lifestyle, M. tuberculosis executes a complex, interconnected web of stress responses that rely on changes in gene expression. In fact, M. tuberculosis is well suite...

show abstract

“…The essential transcription initiation factors CarD (Rv3583c) and RbpA (Rv2050) play important roles in the biology of M. tuberculosis. [39][40][41][42] Initiation requires the formation of RNA polymerase (RNAP) holoenzyme with the bound sigma factors. A series of kinetic steps bring about the formation of a closed complex leading to the open complex with the transcription bubble.…”

Section: Mycobacterium Tuberculosis: a Closer Lookmentioning

confidence: 99%

Early diagnosis and effective treatment regimens are the keys to tackle antimicrobial resistance in tuberculosis (TB): A report from Euroscicon's international TB Summit 2016

et al. 2016

View full text Add to dashboard Cite

To say that tuberculosis (TB) has regained a strong foothold in the global human health and wellbeing scenario would be an understatement. Ranking alongside HIV/AIDS as the top reason for mortality due to a single infectious disease, the impact of TB extends far into socio-economic context worldwide. As global efforts led by experts and political bodies converge to mitigate the predicted outcome of growing antimicrobial resistance, the academic community of students, practitioners and researchers have mobilised to develop integrated, inter-disciplinary programmes to bring the plans of the former to fruition. Enabling this crucial requirement for unimpeded dissemination of scientific discovery was the TB Summit 2016, held in London, United Kingdom. This report critically discusses the recent breakthroughs made in diagnostics and treatment while bringing to light the major hurdles in the control of the disease as discussed in the course of the 3-day international event. Conferences and symposia such as these are the breeding grounds for successful local and global collaborations and therefore must be supported to expand the understanding and outreach of basic science research.

show abstract

Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA

Cited by 51 publications

References 39 publications

The essential activities of the bacterial sigma factor

The essential activities of the bacterial sigma factor

Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks

Early diagnosis and effective treatment regimens are the keys to tackle antimicrobial resistance in tuberculosis (TB): A report from Euroscicon's international TB Summit 2016

Contact Info

Product

Resources

About