Structural features and biological activities of the polysaccharides from Astragalus membranaceus

Jin, Mingliang; Zhao, Ke; Huang, Qingsheng; Shang, Peng

doi:10.1016/j.ijbiomac.2013.12.002

Cited by 258 publications

(170 citation statements)

References 110 publications

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“…It exerts important functions as a tonic and diuretic to treat weakness, anemia, and chronic fatigue [16,17]. Certain studies show it functioning as an anti-inflammatory and antioxidant agent [18,19]. Astragaloside IV (AGIV) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Astragaloside IV Improves Vasodilatation Function by Regulating the PI3K/Akt/eNOS Signaling Pathway in Rat Aorta Endothelial Cells

et al. 2018

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Coronary heart disease (CHD) remains a major public health burden. Endothelial-dependent coronary artery vasoreactivity is a significant indicator of vascular function. Endothelial dysfunction is characterized by decreased nitric oxide (NO) bioavailability and predicts late cardiovascular events. Astragaloside IV (AGIV) is the main active component of the herb Astragalus membranaceus. Although it shows a significant protective effect against vascular endothelial dysfunction, the mechanisms of AGIV promoting the vascular dilation have not been elucidated. This study investigated the vasodilator effect of AGIV on rat aortic rings and the underlying effect of AGIV via the PI3K/Akt/eNOS signaling pathway. We measured the relaxation of isolated RARs after different concentrations of AGIV treatment. Rat aorta endothelial cells were cultured with different doses of AGIV, dimethylsulfoxide, and NG-nitro L-arginine methyl ester. The expression of phosphorylated (p)-Akt and -endothelial nitric oxide synthase (p-eNOS) were tested by Western blot analysis. The messenger (m)RNA expression of eNOS was quantified by real-time polymerase chain reaction. AGIV exerted a vasodilator effect on the aortic rings and increased the NO content in a concentration-dependent manner. The vasorelaxation was suppressed by an eNOS inhibitor. AGIV regulated the PI3K/Akt/eNOS signaling pathway via phosphorylation of Akt at Ser⁴⁷³ and dephosphorylation of eNOS at Thr⁴⁹⁵. The mRNA expression of eNOS was remarkably upregulated by AGIV. AGIV significantly induced the dilation of the aortic rings, leading to the vasodilator response by enhancing the eNOS release via the PI3K/Akt/eNOS signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Astragaloside IV Improves Vasodilatation Function by Regulating the PI3K/Akt/eNOS Signaling Pathway in Rat Aorta Endothelial Cells

et al. 2018

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“…The activity of polysaccharides might be caused and influenced by their glycosidic linkages, chain length, the number of branched points, molecular size and tertiary structure (Raveendran Nair et al, 2004). The unique structure diversities and physiochemical properties can be utilized successfully in various medical applications, and many polysaccharides have shown promising potential as antitumor agents, such as ␤-glucans, amylopectin-like polysaccharides and pectins (Brown, 2006;Cao, 2013;Jin, Zhao, Huang, & Shang, 2014;Silva et al, 2012;Sun, 2011). These polysaccharides usually have low toxicity and few side effects, which make them appropriate for immunotherapy against tumors.…”

Section: Introductionmentioning

confidence: 99%

Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry

Wang

Gao

et al. 2015

Carbohydrate Polymers

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“…Milkvetch root has a variety of biological effects: it is a good immunomodulator [33][34][35], regulates T-lymphocyte function, inhibits nuclear factor-ț% Dctivation, increases the secretory immunoglobulin A content in respiratory mucosa, controls humoral immunity, reduces inflammatory edema of the bronchial mucosa, and controls clinical symptoms in patients with COPD. Milkvetch root suppresses the production of oxygen free radicals, prevents their interaction with membrane lipids to generate lipid peroxides, reduces damage to the biomembrane [36], and suppresses platelet adhesion.…”

Section: Jiang Et Al / Milkvetch Root Improves Immune Function Inmentioning

confidence: 99%

Milkvetch root improves immune function in patients with acute exacerbation of COPD

Jiang

Wang

et al. 2015

BME

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Abstract. Milkvetch root as a medicine has been used for more over 2000 years in China, can strengthen immune function, protect liver, promote urination, resist aging and stress, reduce blood pressure and extensively resist bacterium. This study explored the effects of milkvetch root on the immune function of patients with a definitive diagnosis of acute exacerbation of chronic obstructive pulmonary disease (COPD). The patients were randomly assigned to either the experimental or control group. All patients received conventional clinical therapy; those in the experimental group were also administered milkvetch root. The serum levels of cytokines including tumor necrosis factor alpha (TNF-Į LQWHUOHXNLQ-8 (IL-8), IL-ȕ DQG ,/-32 and immunocytes including T helper (Th), cytotoxic T (Tc), natural killer (NK), regulatory T (Treg) and B cells were measured 1 day before treatment and 7 and 14 days post-treatment. After bronchodilator inhalation, pulmonary function was evaluated at these same time points. The serum TNF-Į ,/-8, IL-1ȕ DQG ,/-32 levels were significantly lower in the experimental group than in the control group 14 days post-treatment. The Th/Tc ratio and NK cell ratio was significantly higher but the Treg cell ratio was significantly lower in the experimental group than in the control group. The forced expiratory volume in 1 second (FEV1) and FEV1/forced vital capacity (FVC) were significantly higher in the experimental group than in the control group 14 days post-treatment. These results indicate that milkvetch root can improve the immune function of patients with acute exacerbation of COPD.

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Structural features and biological activities of the polysaccharides from Astragalus membranaceus

Cited by 258 publications

References 110 publications

Astragaloside IV Improves Vasodilatation Function by Regulating the PI3K/Akt/eNOS Signaling Pathway in Rat Aorta Endothelial Cells

Astragaloside IV Improves Vasodilatation Function by Regulating the PI3K/Akt/eNOS Signaling Pathway in Rat Aorta Endothelial Cells

Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry

Milkvetch root improves immune function in patients with acute exacerbation of COPD

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