Structural characterization of chondroitin sulfate from sturgeon bone

Maccari, Francesca; Ferrarini, Fabrizio; Volpi, Nicola

doi:10.1016/j.carres.2010.05.016

Cited by 50 publications

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“…In a previous study, Maccari et al . incubated defatted sturgeon bones in 100 mmol L −1 sodium acetate buffer (pH 5.5) with papain for 24 h at 60 °C and reported that the content of purified CS was 0.34% for dry tissue.…”

Section: Resultsmentioning

confidence: 99%

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Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

et al. 2013

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Section: Resultsmentioning

confidence: 99%

“…The signals at 102.1 and 104.4 ppm were assigned to C1 of GalNAc‐6SO 4 and to C1 of GlcA respectively, while the signals at 101.5 and 103.5 ppm were assigned to C1 of GalNAc‐4SO 4 and to C1 of GlcA respectively. Moreover, the signal at 67.5 ppm was related to C6 of GalNAc‐6SO 4 …”

Section: Resultsmentioning

confidence: 99%

Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

et al. 2013

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“…This characteristic feature was be yond doubt taking into account the sources of the polysac charides under study and the fact that their NMR spectra are identical to those published earlier for chondroitin sulfates. 22, 23 An analysis of the integrated intensities of the signals in the 1 H NMR spectra, to be more precise, the intensity ratios of the proton H(4 F ) and the protons H(2 B ) + H(2 D ) + + H(2 T ), provides an estimate of the chain length of the polysaccharide products (Table 3). It can be seen that chondroitin sulfates isolated from different sources differ in the chain length.…”

Section: The Block Structure Of the Chain Was Confirmed By The Absencmentioning

confidence: 99%

Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage

et al. 2011

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Chondroitin sulfates isolated from cartilage of five marine fish species: Atlantic salmon (Salmo salar), Greenland shark (Somniosus microcephalus), blackmouth catshark (Galeus melas tomus), birdbeak dogfish (Deania calcea), and Arctic skate (Amblyraja hyperborea), were char acterized in detail by 1 H and 13 C NMR spectroscopy. The complete signal assignments for carbohydrates were made and the relative contents of the key structural units were estimated by 2D homonuclear and heteronuclear NMR spectroscopy (COSY, TOCSY, NOESY, HSQC, and HMBC). The average length of the polysaccharide chain was evaluated from the integrated intensity ratio of the terminal and internal monosaccharide residues. The anti inflammatory and anticoagulant activities of the specimens were studied. Chondroitin sulfates from salmon and Arctic skate exhibit considerable anti inflammatory activity. All specimens manifest weak anticoagulant activity. The results of the present study indicate that chondroitin sulfates deserve more detailed investigation as potential anti inflammatory agents.

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“…The inspection of the 50 to 70 and 100 to 110 ppm regions revealed a relatively high amount of CS/DS sulfated in position 4 or 6 of the GalNAc. 7,39 In the 13 C NMR spectra, two anomeric carbon signals at 104.1 and 102.08 ppm were attributed to GlcA and GalNAc, respectively. Indeed, the signals at 104.1 and 102.08 were assigned to C1 of GlcA and to C1 of GalNAc-6SO 4 , respectively, and the signals at 104.03 and 101.11 were assigned to C1 of GlcA and to C1 of GalNAc-4SO 4 , respectively.…”

Section: Nmr Analysismentioning

confidence: 99%

Purification and structural elucidation of chondroitin sulfate/dermatan sulfate from Atlantic bluefin tuna (Thunnus thynnus) skins and their anticoagulant and ACE inhibitory activities

et al. 2018

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Chondroitin sulfate/dermatan sulfate (CS/DS) was extracted from Atlantic bluefin tuna (Thunnus thynnus) skin (SGAT) and was purified and characterized. SGAT was characterized by acetate cellulose electrophoresis, FTIR spectroscopy, 13 C NMR spectroscopy and SAX-HPLC. According to the results obtained for specific chondroitinases (ABC and AC) and the SAX-HPLC separation of generated unsaturated repeating disaccharides, the polymer was found to contain a disaccharide monosulfated in positions 6 and 4 of GalNAc and disulfated disaccharides in different percentages. These results were confirmed by 13 C NMR experiments. The average molecular mass was 24.07 kDa, as determined by PAGE analysis. SGAT was evaluated for its in vitro anticoagulant activity via activated partial thromboplastin time, thrombin time and prothrombin time tests. The polymer showed strong inhibitory activity against angiotensin I-converting enzyme (IC 50 ¼ 0.25 mg mL À1 ). Overall, the results suggest that this newly extracted CS/DS can be useful for pharmacological applications. Fig. 3 (A) Anticoagulant activity of the purified CS/DS from Atlantic bluefin tuna skin (SGAT) at different concentrations evaluated by measurement of the activated partial thromboplastin time (aPTT). (B) Anticoagulant activity of SGAT at different concentrations evaluated by measurement of the thrombin time (TT). (C) Anticoagulant activity of SGAT at different concentrations evaluated by measurement of the prothrombin time (PT). All results are expressed as mean AE SD (n ¼ 3) and all measurements were performed in triplicate. 37972 | RSC Adv., 2018, 8, 37965-37975 This journal is

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Structural characterization of chondroitin sulfate from sturgeon bone

Cited by 50 publications

References 22 publications

Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage

Purification and structural elucidation of chondroitin sulfate/dermatan sulfate from Atlantic bluefin tuna (Thunnus thynnus) skins and their anticoagulant and ACE inhibitory activities

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