Structural characterization of a pectic polysaccharide from laoshan green tea and its inhibitory effects on the production of NO, TNF-<i>α</i> and IL-6

Liu, Fan; Wang, Rongshen; Chen, Ying; Geng, Rui; Gao, Hong; Wang, Feng; Liu, Huan; Li, Wanzhong

doi:10.1080/14786419.2022.2121831

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…Polysaccharides, complex biopolymers consisting of 10 or more monosaccharides linked by glycosidic bonds, stand as some of nature’s most prevalent molecules [ 1 ]. Research on polysaccharides is expanding due to their potent anti-tumor [ 2 ], hypoglycemic [ 3 ], antioxidant [ 4 ], anti-allergic [ 5 ], and immunomodulatory [ 6 ] effects, and their capacity to shield cells from inflammatory cytokines [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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Polysaccharides extracted from Taxus media hrough an aqueous method were further refined by removing proteins via the Sevag technique and purified by dialysis. The separation of these polysaccharides was accomplished using a DEAE-cellulose chromatog-raphy column, yielding two distinct fractions, named CPTM-P1 and CPTM-P2. Notably, CPTM-P1 emerged as the primary polysaccharide component within Taxus media. Consequently, a comprehensive analysis focusing exclusively on CPTM-P1 was undertaken. The molecular weight of CPTM-P1 was established through gel permeation chromatography (GPC), and its monosaccharide composition was deciphered using HPLC-MS. The structure was further elucidated through nuclear magnetic resonance (NMR) spectroscopy. The molecular weight of CPTM-P1 was determined to be 968.7 kDa. The monosaccharide composition consisted of galactose (Gal), arabinose (Ara), galacturonic acid (Gal-UA), glucose (Glc), rhamnose (Rha), xylose (Xyl), mannose (Man), fucose (Fuc), glucuronic acid (Glc-UA), and ribose (Rib). The proportional distribution of these components was 30.53%, 22.00%, 5.63%, 11.67%, 11.93%, 1.69%, 8.50%, 1.23%, 5.63%, and 1.17%, respectively. This confirmed CPTM-P1 as an acidic heteropolysaccharide with a glycuronic acid backbone. Moreover, CPTM-P1 showed immunoenhancing properties, effectively augmenting the secretion of nitric oxide and cytokines (TNF-α, IL-1β, and IL-6). Additionally, it significantly enhances the phagocytic capacity of RAW264.7 cells. These findings underscore the potential application of these polysaccharides in functional foods and pharmaceuticals, providing a solid scientific basis for further exploration and utilization of Taxus media polysaccharides.

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Section: Introductionmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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“…In recent years, natural bioactive compounds have received much attention from researchers for their effective hypolipidemic and nephroprotective actions, as well as their minimal side effects. , Green tea, a well-known functional and flavored beverage, has long been used to prevent various metabolic disorders . Polysaccharides are recognized as key bioactive ingredients in green tea, possessing health-promoting properties such as antioxidative, anti-inflammatory, and hypolipidemic effects. − Previously, we isolated and purified a homogeneous tea polysaccharide named TPS3A (Figure S1), whose chemical structure was characterized as a repeating unit consisting of 1,3-Galp, 1,4-Galp, 1,3,6-Galp, 1,3-Araf, 1,5-Araf, 1,2,4-Rhap, and 1-GalpA, with two branches on the C6 of 1,3,6-Galp and C2 of 1,2,4-Rhap, respectively . Importantly, TPS3A exhibited remarkable benefits against a HFD-induced hyperlipidemia.…”

Section: Introductionmentioning

confidence: 99%

Tea Polysaccharide Ameliorates High-Fat Diet-Induced Renal Tubular Ectopic Lipid Deposition via Regulating the Dynamic Balance of Lipogenesis and Lipolysis

Kuang,

Li,

Qian

et al. 2024

J. Agric. Food Chem.

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Renal tubular ectopic lipid deposition (ELD) plays a significant role in the development of chronic kidney disease, posing a great threat to human health. The present work aimed to explore the intervention effect and potential molecular mechanism of a purified tea polysaccharide (TPS3A) on renal tubular ELD. The results demonstrated that TPS3A effectively improved kidney function and slowed the progression of tubulointerstitial fibrosis in high-fat-diet (HFD)-exposed ApoE −/− mice. Additionally, TPS3A notably suppressed lipogenesis and enhanced lipolysis, as shown by the downregulation of lipogenesis markers (SREBP-1 and FAS) and the upregulation of lipolysis markers (HSL and ATGL), thereby reducing renal tubular ELD in HFD-fed ApoE −/− mice and palmitic-acid-stimulated HK-2 cells. The AMPK-SIRT1-FoxO1 axis is a core signal pathway in regulating lipid deposition. Consistently, TPS3A significantly increased the levels of phosphorylated-AMPK, SIRT1, and deacetylation of Ac-FoxO1. However, these effects of TPS3A on lipogenesis and lipolysis were abolished by AMPK siRNA, SIRT1 siRNA, and FoxO1 inhibitor, resulting in exacerbated lipid deposition. Taken together, TPS3A shows promise in ameliorating renal tubular ELD by inhibiting lipogenesis and promoting lipolysis through the AMPK-SIRT1-FoxO1 signaling pathway.

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Structural characterization of a pectic polysaccharide from laoshan green tea and its inhibitory effects on the production of NO, TNF-α and IL-6

Cited by 2 publications

References 35 publications

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Tea Polysaccharide Ameliorates High-Fat Diet-Induced Renal Tubular Ectopic Lipid Deposition via Regulating the Dynamic Balance of Lipogenesis and Lipolysis

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