Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Abstract

This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1 H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% ␣-(1,6) linkages in the main chains and 2.1% ␣-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% ␣-(1,6) linkages in the main chains and 5% ␣-(1,3) branch linkages.

Structural analysis and characterization of a clinically important low molecular weight natural dextran synthesized by Leuconostoc lactis KU665298 dextransucrase

“…In a previous study, the -1,6-glycosidic linkage in the main chains was demonstrated at a peak of 4.93 ppm, whereas -1, 3-glycosidic link-age in the branch is located at approximately 5.20 ppm (Gan et al, 2014). Similar results have been reported by other authors (Seymour et al, 1979;Vettori et al, 2012;Dong et al, 2013).…”

“…05, 97.70, 73.39, 71.39, 69.18 and 65.53 ppm (Figures 8 a and b), which was a characteristic of linear dextran. The major resonance in the anomeric region occurs at 97.70 ppm showed that the C-1 is linked (Vettori et al, 2012). Another intense signal at 62.65 confirmed that most of C-6 were also linked, and this proved that glucose residues in dextran were linked by -(1,6) glycosidic bond.…”

“…Dextran is an example of bacterial homopolysaccharide composed of D-glucose and largely of -1,6-glucopyranosidic linkages in the main chain (Naessens et al, 2005;Vettori et al, 2012;Liu et al, 2017;Nacher-Vazquez et al, 2017). Lactic acid bacteria have a long history of safe use in human consumption and, therefore, among variety of microorganisms, are most widely explored for the production of microbial exopolysaccharides (Nikolic et al, 2012).…”

Water soluble cationic dextran derivatives containing poly(amidoamine) dendrons for efficient gene delivery

“…S1), its number-average molecular weight (M n ) and molecular weight distribution (M w /M n ) were determined to be 33,489 and 1.55, respectively. By 1 H NMR analysis (Benzeval, Bowyer, & Hubble, 2012;Pasika & Cragg, 1963;Vettori, Franchetti, & Contieroa, 2012), its degree of ␣-(1,3)-linked branching was found to be 5.0% (Fig. S2).…”

Methods for Generating Hydrogel Particles for Protein Delivery

“…67 It has been used therapeutically as a blood thinner to decrease thrombosis. 6 Microstructured dextran-based hydrogels that significantly reduced the burst effect in releasing the PEGylated protein drug interferon-α2a were reported by Bae et al; microstructured PEG domains carrying the protein drug were dispersed throughout a bulk dextran hydrogel.…”