Structural Characterization and Biological Functions of Fibroblast Growth Factor

Gospodarowicz, Denis; Ferrara, Napoleone; Schweigerer, Lothar; Neufeld, Gera

doi:10.1210/edrv-8-2-95

Cited by 1,233 publications

(602 citation statements)

References 93 publications

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“…[51,52] However, high concentrations of heparin inhibit the proliferation of smooth muscle cells. [53] The bFGF bioactivity from PEUU scaffolds without heparin indicated that commonly-used growth factor stabilizing protein BSA [20] alone could stabilize the bFGF and maintain smooth muscle cell mitogenicity. The incorporation of heparin may further stabilize the bFGF, but our data did not suggest such stabilization.…”

Section: Discussionmentioning

confidence: 99%

“…Thermally induced phase separation was used to prepare a highly porous scaffold and a bFGF-containing protein complex was directly loaded into the polymer during scaffold preparation. Heparin, known to stabilize bFGF by inducing a conformational change in the molecule and increasing its resistance to denaturation [19][20][21] was examined as an additive, while bovine serum albumin (BSA) was used as a stabilizing protein. Scaffold morphology, mechanical properties, bFGF release kinetics and bioactivity, scaffold degradation and in vitro cell culture within the scaffold were investigated.…”

Section: Introductionmentioning

confidence: 99%

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Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

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Scaffolds that better approximate the mechanical properties of cardiovascular and other soft tissues might provide a more appropriate mechanical environment for tissue development or healing in vivo. An ability to induce local angiogenesis by controlled release of an angiogenic factor, such as basic fibroblast growth factor (bFGF), from a biodegradable scaffold with mechanical properties more closely approximating soft tissue could find application in a variety of settings. Toward this end biodegradable poly(ester urethane)urea (PEUU) scaffolds loaded with bFGF were fabricated by thermally induced phase separation. Scaffold morphology, mechanical properties, release kinetics, hydrolytic degradation and bioactivity of the released bFGF were assessed. The scaffolds had interconnected pores with porosities of 90% or greater and pore sizes ranging from 34-173 μm. Scaffolds had tensile strengths of 0.25-2.8 MPa and elongations at break of 81-443%. Incorporation of heparin into the scaffold increased the initial burst release of bFGF, while the initial bFGF loading content did not change release kinetics significantly. The released bFGF remained bioactive over 21 days as assessed by smooth muscle mitogenicity. Scaffolds loaded with bFGF showed slightly higher degradation rates than unloaded control scaffolds. Smooth muscle cells seeded into the scaffolds with bFGF showed higher cell densities than for control scaffolds after 7 days of culture. The bFGFreleasing PEUU scaffolds thus exhibited a combination of mechanical properties and bioactivity that might be attractive for use in cardiovascular and other soft tissue applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

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“…Binds to its receptor FGFR Stimulates in vitro and in vivo angiogenesis 92,145,417 Stimulates endothelial cell proliferation 148 Enhances endothelial cell migration 145,149 Stimulates the production of matrix proteases in endothelial cells 147 …”

Section: Fgf Fibroblast Growth Factormentioning

confidence: 99%

The Role of Mechanical Stresses in Angiogenesis

Shiu

Weiss

Hoying

et al. 2005

Crit Rev Biomed Eng

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ABSTRACT:Angiogenesis is the formation of new capillary blood vessels from preexisting vessels. It is involved in many normal and diseased conditions, as well as in the application of tissue-engineered products. Th ere has been extensive eff ort made to develop strategies for controlling pathological angiogenesis and for promoting vascularization in biomedical engineering applications. Central to advancing these strategies is a mechanistic understanding of the angiogenic process. Angiogenesis is tightly regulated by local tissue environmental factors, including soluble molecules, extracellular matrices, cell-cell interactions, and diverse mechanical forces. Great advances have been made in identifying the biochemical factors and intracellular signaling pathways that mediate the control of angiogenesis. Th is review focuses on work that explores the biophysical aspect of angiogenesis regulation. Specifi cally, we discuss the role of cell-generated forces, counterforces from the extracellular matrix, and mechanical forces associated with blood fl ow and extravascular tissue activity in the regulation of angiogenesis. Because angiogenesis occurs in a mechanically dynamic environment, future investigations should aim at understanding how cells integrate chemical and mechanical signals so that a rational approach to controlling angiogenesis will become possible. In this regard, computational models that incorporate multiple epigenetic factors to predict capillary patterning will be useful.

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“…It (Gospodarowicz et al, 1987 (Esch et al, 1985), while an inhibition of differentiation was shown with a more purified bFGF preparation.…”

mentioning

confidence: 97%

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁

Nataf

Tsagris²,

Dumontier³

et al. 1990

Reprod. Nutr. Dévelop.

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Structural Characterization and Biological Functions of Fibroblast Growth Factor

Cited by 1,233 publications

References 93 publications

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

The Role of Mechanical Stresses in Angiogenesis

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁

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Structural Characterization and Biological Functions of Fibroblast Growth Factor

Cited by 1,233 publications

References 93 publications

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

The Role of Mechanical Stresses in Angiogenesis

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF1

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Product

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Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁