Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

Abstract

Dandelion (Taraxacum mongolicum Hand.-Mazz.) is a medicinal and edible plant. Dandelion has great development value for its health promoting benefits; additionally, Dandelion grows almost anywhere in the world. In this study, we report the structural characteristics and anti-cancer activity of novel dandelion leaf polysaccharides extracted by pressurized hot water extraction at 120 °C (DLP120) with Mw relative to dextran of 1.64 × 106 Da. Structural analysis indicated that DLP120 is a complex polysaccharide composed of pectin and arabinogalactan. It was mainly composed of arabinose (32.35 mol%) and galactose (44.91 mol%). The main glycosidic linkages of DLP120 were 4-β-D-Galp, 4-α-D-GalpA, T-β-D-Galp, 5-α-L-Araf, 3,5-α-L-Araf, and T-α-L-Araf. In vitro, DLP120 inhibited HepG2 cell proliferation in a dose-dependent manner by inducing cell apoptosis. Cell cycle detection results revealed that DLP120 mainly arrests the cell cycle in S phase. Cells treated with DLP120 displayed obvious apoptotic morphology, including cell volume shrinks and cytoskeleton breaks down. In short, DLP120 has potential as an anti-cancer agent.

Comparison of Polygonatum sibiricum Polysaccharides Found in Young and Mature Rhizomes

“…The hydroxyl and carboxyl groups of polysaccharide, which are crucial for the total antioxidant ability, can directly interact with phosphorus molybdenum reagent and promote their reduction. So, the quantity and density distribution of the above two functional groups can directly influence the total antioxidant ability of the polysaccharide [31]. Therefore, it is possible that the PsP of young rhizome possesses less quantity and lower density distribution of hydroxyl and carboxyl groups according to our result.…”

“…Detection was achieved using a differential refractive index detector, with a flow rate set at 0.5 mL/min and a mobile phase comprising 0.05 M sodium sulfate. The chromatographic column was calibrated utilizing a range of distinct dextran standards to generate a standard curve [31].…”

A New Method of Extracting Polygonatum sibiricum Polysaccharide with Antioxidant Function: Ultrasound-Assisted Extraction-Deep Eutectic Solvents Method

“…The linear correlation was established by using log (Mw) as abscissa and retention time as ordinate [ 34 ].…”

Senzorska procjena tjestenine obogaćene maslačkom (Taraxacum officinale Weber)

“…Istri (plominsko zelje) (Vitasović-Kosić, 2018) i koristi se kao svježe lisnato povrće (Łuczaj et al, 2013). Često je korišten kao lijek u narodnoj medicini zbog povoljnog djelovanja na jetru, a do danas su istraživana njegova diuretska, protuupalna, antioksidativna, antihiperglikemijska, prebiotska (Schütz et al, 2006;Wirngo et al, 2016;Pierzak-Sominka, 2020), antikancerogena (Pierzak-Sominka, 2020; Jalili et al, 2020;Chen et al, 2023), hepatoprotektivna, neuroprotektivna, genoprotektivna Mahboubi, 2020;Jalili et al, 2020) i druga svojstva, a upravo zbog protuupalnih, Emilija Friganović, Duška Ćurić, Tajana Krička / Senzorska procjena tjestenine obogaćene maslačkom (Taraxacum officinale Weber) / Glasilo Future ( 2023) 6 (2-3) 32-43 antioksidativnih i antihiperglikemijskih svojstava okarakteriziran je kao antidijabetička i medicinska biljka (Wirngo et al, 2016;Pierzak-Sominka, 2020;Jalili et al, 2020).…”