Structural Changes Induced by Potassium Dichromate in Renal Cortex of Adult Male Albino Rats and the Possible Protective Role of Selenium

Abstract

Background: Potassium Dichromate (Cr) is a common environmental agent that has been considered potentially carcinogenic, making it an issue of major environmental and search concern. Aim of Study: The aim of this study was to throw light on the possible structural changes in the renal cortex of adult male albino rats after administration of Cr and to elucidate the possibility of protection by selenium (Se) against the deleterious effects of Cr. Material and Methods: Thirty six adult male albino rats weighing 200-230g were divided equally into four groups as follow: Group I received balanced diet and tap water only; Group II received Se orally 0.25mg/kg b.w/daily; Group III rats were received Cr orally 67mg/kg/daily and Group IV rats were received oral Cr and Se at same previous doses. Animals were sacrificed on day 28. Results: The Cr-treated group showed damage of the renal cortex including capsule and renal interstitium. Masson's trichrome staining showed abundant collagen fibers. The expression of caspase-3 in the cytoplasm showed strong positive immune reaction in group III. Biochemical results showed Cr induced significant increase in blood urea nitrogen (BUN), serum creatinine (SC) and uric acid (UA). While morphometric measurement indicated that Cr induced a statistical significant decrease in rat body weight and diameter of glomeruli when compared with other groups. The Cr-treated group also showed a statistical significant increase in kidney weight, surface area of Bowman space, diameter of the convoluted tubules, area percentage of collagen fibers and expression of caspase-3. Meanwhile, administration of Se showed some protection against the toxic effect of Cr on renal cortex. Conclusion: It is suggested that administration Se moderately improves the deleterious histological and biochemical changes induced by Cr on renal cortex.

Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-Formulation against Nephrotoxicity in Rats as Revealed via Biochemical, Histopathological, and UPLC-QTOF–MS/MS Analyses

“…The renal cortex had enlarged renal corpuscles with a wide renal space, tubular degeneration, and proximal convoluted tubules which displayed vacuolar degeneration with a luminal cast, and distal convoluted tubules which showed the complete loss in the cytoplasmic content with the sloughing of the lining cuboidal cells into the tubular lumen. These findings are in accordance with previous reports [ 65 , 66 , 67 ], which were suggestive that K 2 Cr 2 O 7 -induced toxicity and the induction of oxidative stress and apoptosis arise through the generation of reactive oxygen species (ROS). Consequently, ROS triggered K 2 Cr 2 O 7 toxicity owing to the reduction of hexavalent chromium to the trivalent form, thereby inducing damage to the cellular components [ 68 ].…”

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

“…The current study revealed little collagen fibers in the cortical interstitium around the renal corpuscles and tubules in Masson's trichrome stained specimens of protected groups. The results agreed with Ahmed et al, (2019) [45] . The (PFOS) administered group in the present study exhibited significant increase of iNOS immunohistochemical reaction.…”

Protective Effect of Lactoferrin against Chromium Induced Adverse Renal Changes in Rats: Oxidative Stress Theory

“…They also stated that the treated animals showed intraluminal and intracytoplamic accumulation of acidophilic hyaline and renal cast content (Hegazy et al, 2016). Also other studies showed similar findings (El-Mahalaway et al, 2015;Hanan et al, 2019).…”