Structural changes in the proximal tubule of kidneys from hypophysectomized rats

Abstract

The present study was undertaken to determine what structural changes hypophysectomy would cause in the rat kidney. Proximal convoluted tubule cells from hypophysectomized rats were smaller and their length was decreased when compared to kidneys from control animals of approximately the same weight as the hypophysectomized animals. Electron microscopic observation of the kidneys from the hypophysectomized rats revealed that the cells of the proximal convoluted tubules had ( 1 ) an increase in size and number of dense bodies (type I1 cytosome); (2) a decrease in smooth endoplasmic reticulum (SER); ( 3 ) a decrease in basilar infoldings; (4) an increase in the frequency of mitochondria cristae aligned in their longitudinal axis and (5) a change in the character of the proses of smooth endoplasmic reticulum (SER) related to microbodies (type I cytosome) from a vesicular to tubular arrangement. The changes noted in the cells of the proximal convoluted tubules of hypophysectomized rats were similar to previously described changes observed in tubular a trophy.

Differences in Rat Kidney Morphology between Males, Females and Testosterone-Treated Females

“…with a higher filtrate) [25,26]. After hypophysectomy (which is followed by the cessation of androgen secretion) both, the size of the PTs (epithelial area and tubular length) and the GFR are diminished [27].…”

Renal Response to Infusion of Isotonic Ringer-Locke Solution: Effect of Hypophysectomy

“…Loss of kidney weight, however, was relatively greater than loss of body weight after 2 months of hypophysectomy, since the slope relating kidney to body weight was greater in normal than in hypophysectomized rats. Relative stability ofthis atrophy 2 months after surgery has been previously reported (Evan et al 1972). Table 1 shows the results of the analysis of the ECF compartment before and after ECF volume expansion in normal and hypophysectomized rats.…”

“…Previous work (Evan et al 1972) suggests that specific atrophy of the proximal tubule occurs after hypophysectomy, and that this state of atrophy appears to stabilize approximately 8 weeks after hypophysectomy. Characteristics of proximal tubular atrophy following hypophy¬ sectomy are (a) reduction in the brush border height and density, (b) decreased extent of basal infoldings, (c) decrease in cell size and (d) a reduction in tubular length.…”

“…Hypo¬ physectomized rats were given hypophysectomized rat diet (Shaw «fe Greep, 1949) obtained from ICN Nutritional Biochemicals (Cleveland, Ohio). Hypophysectomized rats were kept for 2 months before the experiments to ensure relatively uniform and stable atrophy of the proximal tubular epithelium (Evan et al 1972).…”

“…The distal tubule apparently remains unchanged. These structural changes in the proximal tubule appear to stabilize approximately 2 months after hypophysectomy in rats (Evan et al 1972) and are characteristic of tubular atrophy (Chatelant «fe Simon, 1969).…”