Structural changes in fibrin clot associated with the proteolytic activity induced by tissue type plasminogen activator. An NMR study

Azoury, R.; Bitton, M.; Abrashkin, S.; Lavie, E.

doi:10.1016/0167-4838(89)90050-2

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…In recent years, further areas have been added to the list such as face lifts, oncologic surgery, plastic surgery, treatment of burns, etc. Much research has been focused on fibrin both as a hemostatic barrier and wound‐healing agent 7–11…”

Section: Introductionmentioning

confidence: 99%

An in vivo study of hydrogels based on physiologically clotted fibrin–gelatin composites as wound‐dressing materials

Eusuff

Sastry

2004

J Biomed Mater Res

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In this study we have reported the efficacy of three biomaterials: (a) physiologically clotted fibrin-gelatin composite (PFG), (b) PFG graft copolymerized with 2-hydroxyethyl methacrylate (PFG-HEMA), and (c) PFG graft copolymerized with 2-hydroxypropyl methacrylate (PFG-HPMA) as temporary wound-dressing materials using the rat as an animal model. Full-thickness excision wounds were made on the back of female rats weighing about 150 +/- 10 g. The dressings were applied on the wounds and changed periodically at an interval of 4 days with the respective materials. The wounds treated with PFG-HEMA healed completely on 15th day after wound creation, whereas those treated with PFG and PFG-HPMA resulted in complete healing on the 17th day. The concentrations of collagen, hexosamine, and uronic acid in the granulation tissue were determined. The PFG and its graft copolymers acted as hydrogels, thereby absorbing excess exudates, while still maintaining a moist environment at the wound site. The enhanced wound healing in the experimental animals was reflected in the increased rate of wound contraction. The results of the histological and mechanical studies of the experimental groups revealed that reepithelialization and remodeling of the skin have been achieved by providing a moist environment at the wound site by the biomaterials and thereby hastening the migration of keratinocytes.

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Section: Introductionmentioning

confidence: 99%

An in vivo study of hydrogels based on physiologically clotted fibrin–gelatin composites as wound‐dressing materials

Eusuff

Sastry

2004

J Biomed Mater Res

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“…11,14 In addition, the stability of fibrin clots in the presence of plasmin has been extensively studied. [17][18][19][20][21][22] As a result, evidence both supporting and disproving increased fibrin stability in response to crosslinking exists, forming a debate which has led some researchers to consider the effect of the extent of crosslinking on fibrin stability. 8 Although the protein and platelet interactions with crosslinked fibrin-coated surfaces produced using the methods described in this work have also been investigated, 23 the stability of the coating on a material has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Plasmin degradation of fibrin coatings on synthetic polymer substrates

Bense

Woodhouse

1999

J. Biomed. Mater. Res.

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The goal of this research was to evaluate the in vitro stability of fibrin coatings on polymeric materials in the presence of plasmin. Factor XIIIa-crosslinked and noncrosslinked fibrin layers were coated on three different polyurethane substrates: Corethane, Tegaderm, and a biodegradable polyurethane, PCL/HDI/Phe. Degradation assays indicated that crosslinking the fibrin coatings enhanced the stability of the coatings on both Tegaderm and PCL/HDI/Phe; however, the persistence of the coating on the woven Corethane was not influenced by crosslinking. Degradation assay results also showed that the fibrin coating on the Corethane was significantly less stable than the fibrin coatings on the Tegaderm and PCL/HDI/Phe films. The chromogenic substrate assay data showed crosslinking did not affect the specific plasmin activity on the coatings; therefore, the increased stability resulting from crosslinking was not achieved through a reduction of fibrinolysis. The plasmin activity on the coated Corethane samples was much greater than that on either of the coated flat wound dressing materials. The large surface area of Corethane, a porous woven vascular graft material, may have had a direct influence on the fibrinolysis of its coatings by providing a large number of tissue-type plasminogen activator (tPA) binding sites. A thin, crosslinked, fibrin-coated polyurethane provides a theoretically attractive biomaterial for use in a wound dressing application and should be subject to ongoing research.

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Structural changes in fibrin clot associated with the proteolytic activity induced by tissue type plasminogen activator. An NMR study

Cited by 2 publications

References 17 publications

An in vivo study of hydrogels based on physiologically clotted fibrin–gelatin composites as wound‐dressing materials

An in vivo study of hydrogels based on physiologically clotted fibrin–gelatin composites as wound‐dressing materials

Plasmin degradation of fibrin coatings on synthetic polymer substrates

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