Structural Biology of NCAM

Soroka, Vladislav; Kasper, Christina; Poulsen, Flemming M.

doi:10.1007/978-1-4419-1170-4_1

Cited by 21 publications

(15 citation statements)

References 66 publications

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“…NCAM has five Ig-class and two FN domains in its extracellular region and a cytoplasmic domain that differs in size among three principal isoforms (32). The NCAM extracellular region can be modified by polysialylation (PSA-NCAM), which is implicated in mediating synaptic plasticity (33).…”

Section: Neural Cell Adhesion Molecule (Ncam)mentioning

confidence: 99%

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

Sullivan

Kümper

Temple

et al. 2016

Journal of Biological Chemistry

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Establishment of a proper balance of excitatory and inhibitory connectivity is achieved during development of cortical networks and adjusted through synaptic plasticity. The neural cell adhesion molecule (NCAM) and the receptor tyrosine kinase EphA3 regulate the perisomatic synapse density of inhibitory GABAergic interneurons in the mouse frontal cortex through ephrin-A5-induced growth cone collapse. In this study, it was demonstrated that binding of NCAM and EphA3 occurred between the NCAM Ig2 domain and EphA3 cysteine-rich domain (CRD). The binding interface was further refined through molecular modeling and mutagenesis and shown to be comprised of complementary charged residues in the NCAM Ig2 domain (Arg-156 and Lys-162) and the EphA3 CRD (Glu-248 and Glu-264).

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Section: Neural Cell Adhesion Molecule (Ncam)mentioning

confidence: 99%

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

Sullivan

Kümper

Temple

et al. 2016

Journal of Biological Chemistry

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“…The ECD of NCAM is generally believed to be a prerequisite for NCAM cis-dimerization [6]. To demonstrate this directly, the ability of a truncated NCAM-140 devoid of an ICD (NCAM-trunc, Fig.…”

Section: The Ecd Of Ncam Is Sufficient To Allow Cis-dimerization In Lmentioning

confidence: 99%

“…NCAM is predominantly expressed in the nervous system and regulates neuronal and glial development and regeneration, synaptic plasticity, and learning [1][2][3]. These effects depend on NCAM-mediated cell adhesion and intracellular signaling and involve multiple homo-and heterophilic interactions [1,2,[4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…The mode of NCAM homophilic interactions has been debated, and different Ig modules have been implicated in binding [6]. According to a recent model, NCAM is always present on the cell surface as cis-dimers, formed by interactions between IgI and II modules, and the trans-homophilic interactions of the NCAM cisdimers cause NCAM-mediated cell adhesion [6,9] (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…According to a recent model, NCAM is always present on the cell surface as cis-dimers, formed by interactions between IgI and II modules, and the trans-homophilic interactions of the NCAM cisdimers cause NCAM-mediated cell adhesion [6,9] (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

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Direct demonstration of NCAMcis-dimerization and inhibitory effect of palmitoylation using the BRET²technique

et al. 2010

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a b s t r a c tBiological activity of the neural cell adhesion molecule (NCAM) depends on both adhesion and activation of intra-cellular signaling. Based on in vitro experiments with truncated extra-cellular domains, several models describing homophilic NCAM trans-and cis-interactions have been proposed. However, cis-dimerization in living cells has not been shown directly and the role of the cytoplasmic part in NCAM dimerization is poorly understood. Here, we used the bioluminescence resonance energy transfer (BRET 2 ) technique to directly demonstrate that full-length NCAM cishomodimerizes in living cells. Based on BRET 2 50 values we suggest that the intra-cellular part of NCAM inhibits cis-dimerization, an effect mainly dependent on the palmitoylation sites.

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Nectins and Nectin-like molecules drive vascular development and barrier function

et al. 2023

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Structural Biology of NCAM

Cited by 21 publications

References 66 publications

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

Direct demonstration of NCAMcis-dimerization and inhibitory effect of palmitoylation using the BRET²technique

Nectins and Nectin-like molecules drive vascular development and barrier function

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Structural Biology of NCAM

Cited by 21 publications

References 66 publications

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse

Direct demonstration of NCAMcis-dimerization and inhibitory effect of palmitoylation using the BRET2technique

Nectins and Nectin-like molecules drive vascular development and barrier function

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Product

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Direct demonstration of NCAMcis-dimerization and inhibitory effect of palmitoylation using the BRET²technique