Structural Basis of Bifunctional CTP/dCTP Synthase

Guo, Chen-Jun; Zhang, Zherong; Lu, Jia-Li; Zhong, Jiale; Wu, Yu-Fen; Guo, Shu-Ying; Liu, Ji-Long

doi:10.1016/j.jmb.2024.168750

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2024

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Cited by 3 publications

References 67 publications

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Architecture of CTPS filament networks revealed by cryo-electron tomography

Fu,

Guo,

Liu

et al. 2024

Experimental Cell Research

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Architecture of CTPS filament networks revealed by cryo-electron tomography

Fu,

Guo,

Liu

et al. 2024

Experimental Cell Research

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Cytoophidium complexes resonate with cell fates

Li,

Liu

2024

Preprint

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Metabolism is a fundamental characteristic of life. In 2010, we discovered that the metabolic enzyme CTP synthase (CTPS) can assemble a snake like structure inside cells, which we call the cytoophidium. Including CTPS, an increasing number of metabolic enzymes have been found to form cytoophidia in cells. However, the distribution and relationship among cytoophidia formed by different metabolic enzymes remain elusive. Here we investigate five metabolic enzymes that can form cytoophidia, namely Asn1, Bna5, CTPS (ie. Ura7), Glt1, and Prs5 in Saccharomyces cerevisiae. We find that multiple cytoophidia can be assembled into cytoophidium complexes by docking one after another. Glt1 cytoophidia tend to assemble in non-quiescent cells, while CTPS cytoophidia are more abundant in quiescent cells and form complexes with Prs5 and Asn1 cytoophidia. Blocking CTPS cytoophidium assembly can lead to a non-quiescent phenotype and increase the assembly of Glt1 cytoophidia, Bna5 cytoophidia, and cytoophidium complexes. Blocking CTPS cytoophidium assembly also inhibits the NAD biosynthesis pathway, which includes Bna5 and Sir2. Consistent with this result, the non-quiescent phenotype caused by blocking CTPS cytoophidium assembly can be rescued by blocking Glt1 cytoophidium assembly, supplementing nicotinic acid, or overexpressing Sir2. Our results indicate that the assembly of cytoophidium complexes with different compositions resonates with distinct cell fates.

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The Impact of Developmental and Metabolic Cues on Cytoophidium Formation

Zhang,

Liu

2024

IJMS

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The cytoophidium, composed mainly of CTP synthase (CTPS), is a newly discovered dynamic filamentous structure in various organisms such as archaea, bacteria, and humans. These filamentous structures represent a fascinating example of intracellular compartmentation and dynamic regulation of metabolic enzymes. Currently, cytoophidia have been proven to be tightly regulated and highly dynamic, responding rapidly to developmental and metabolic cues and playing a critical role in maintaining cellular homeostasis. In this review, we would like to discuss in detail the characteristics, mechanisms, functions, and potential applications of this conservative but promising organelle.

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Structural Basis of Bifunctional CTP/dCTP Synthase

Cited by 3 publications

References 67 publications

Architecture of CTPS filament networks revealed by cryo-electron tomography

Architecture of CTPS filament networks revealed by cryo-electron tomography

Cytoophidium complexes resonate with cell fates

The Impact of Developmental and Metabolic Cues on Cytoophidium Formation

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