Structural Basis for p53 Lys120-Acetylation-Dependent DNA-Binding Mode

Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine

“…The gene was cloned into the first open reading frame of a pCDF-Duet vector using Gibson Assembly Kit (NEB, Ipswich, MA). [2,3] This vector also contained a U25C mutant of PylT under constitutive expression. [4] To enable the co-translational incorporation of…”

Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA

“…Such single Hoogsteen bps have been observed in DNA:drug 9,12,33, 96--99 and DNA:protein complexes 17--19, 21 and in DNA containing damaged nucleotides by both X-ray crystallography 17,20, 25,26 and NMR 2--4,6,33 spectroscopy. We therefore examined the feasibility of using the IR markers of G-C + Hoogsteen bps obtained for GC 9 at low pH to characterize single G-C + Hoogsteen bps surrounded by Watson-Crick under physiological pH.…”

“…In the studies of Hoogsteen bps in duplex DNA, one will often be interested in the detection of single Hoogsteen bps surrounded by Watson-Crick bps under physiological pH conditions. Such single Hoogsteen bps have been observed in DNA:drug [9,12,33,[96][97][98][99] and DNA:protein complexes [17][18][19]21] and in DNA containing damaged nucleotides by both X-ray crystallography [17,20,25,26] and NMR [2-4,6,33] spectroscopy. We therefore examined the feasibility of using the IR markers of G-C + Hoogsteen bps obtained for GC 9 at low pH to characterize single G-C + Hoogsteen bps surrounded by Watson-Crick under physiological pH.…”

“…Hoogsteen bps often occur in tandem as adjacent TA/AT and CG/GC Hoogsteen bps in DNA:drug [9][10][11][12][13][14][15][16] and DNA:protein [17][18][19]21] complexes. Interestingly, adjacent A-T and G-C + Hoogsteen bps have not been observed to date.…”

“…1). While Hoogsteen bps are a minor species in naked DNA duplexes, they can become the dominant species in DNA:drug [9][10][11][12][13][14][15][16] and DNA:protein [17][18][19][20][21] complexes where they are thought to play roles in DNA recognition [22], in damaged DNA where they are thought to play roles in damage induction, accommodation, and repair; [23,24] and in the active site of Y-family polymerases such as human polymerase iota [25,26] where they are proposed to play roles in damage-bypass during replication.…”