Structural and immunologic cross-reactivity among filarial and mite tropomyosin: Implications for the hygiene hypothesis

Santiago, Helton da Costa; Bennuru, Sasisekhar; Boyd, Alexis; Eberhard, Mark L.; Nutman, Thomas B.

doi:10.1016/j.jaci.2010.11.007

Cited by 72 publications

(80 citation statements)

References 48 publications

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“…Perhaps cross-reactivity between helminth proteins and dust mite, for example, plays a role in the pathogenesis of parasitic infection and allergic sensitization [49]. Santiago et al [49] demonstrated almost 72% homology between filarial and dust mite tropomysins. However, Sangsupawanich et al [50] reports from his cohort that hook worm and mite allergen were independently associated with childhood wheeze.…”

Section: Varicella Tuberculosis and Parasitesmentioning

confidence: 99%

The hygiene hypothesis revisited

Fishbein

Fuleihan

2012

Current Opinion in Pediatrics

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Section: Varicella Tuberculosis and Parasitesmentioning

confidence: 99%

The hygiene hypothesis revisited

Fishbein

Fuleihan

2012

Current Opinion in Pediatrics

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“…Depletion ELISAs were performed as previously described (27,28). Briefly, flat-bottom plates (CoStar, USA) were coated overnight at 37°C with 10 g/well of peptide 1 (EEDESKKKSCGDEGEPKVE), peptide 2 (VTEGGEDKKK), and peptide 3 (EVAEAPPAEAAPA) and then washed and blocked as described above.…”

Section: Methodsmentioning

confidence: 99%

Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis

Menezes‐Souza

Mendes

Gomes

et al. 2014

Clin. Vaccine Immunol.

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Gold standard serological diagnostic methods focus on antigens that elicit a strong humoral immune response that is specific to a certain pathogen. In this study, we used bioinformatics approaches to identify linear B-cell epitopes that are conserved among Leishmania species but are divergent from the host species Homo sapiens and Canis familiaris and from Trypanosoma cruzi, the parasite that causes Chagas disease, to select potential targets for the immunodiagnosis of leishmaniasis. Using these criteria, we selected heat shock protein 83.1 of Leishmania braziliensis for this study. We predicted three linear B-cell epitopes in its sequence. These peptides and the recombinant heat shock protein 83.1 (rHSP83.1) were tested in enzyme-linked immunosorbent assays (ELISAs) against serum samples from patients with tegumentary leishmaniasis (TL) and visceral leishmaniasis (VL) and from dogs infected with Leishmania infantum (canine VL [CVL]). Our data show that rHSP83.1 is a promising target in the diagnosis of TL. We also identified specific epitopes derived from HSP83.1 that can be used in the diagnosis of human TL (peptide 3), both human and canine VL (peptides 1 and 3), and all TL, VL, and CVL clinical manifestations (peptide 3). Receiver operating characteristic (ROC) curves confirmed the superior performance of rHSP83.1 and peptides 1 and 3 compared to that of the soluble L. braziliensis antigen and the reference test kit for the diagnosis of CVL in Brazil (EIE-LVC kit; Bio-Manguinhos, Fiocruz). Our study thus provides proof-of-principle evidence of the feasibility of using bioinformatics to identify novel targets for the immunodiagnosis of parasitic diseases using proteins that are highly conserved throughout evolution.

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“…[116][117][118][119][120][121][122][123][124][125][126][127][128] We have demonstrated that helminth infection can be associated with increased IgE responses to many different allergens, especially those structurally related to parasite antigens. 116 Individuals infected with filarial parasites were shown to have higher levels of IgE against HDM and cockroach extracts (that have several major allergens with homologues in filarial parasites) but not against timothy grass extract, an allergen extract with just a few minor allergen homologues in helminths.…”

Section: -89mentioning

confidence: 99%

“…116 Individuals infected with filarial parasites were shown to have higher levels of IgE against HDM and cockroach extracts (that have several major allergens with homologues in filarial parasites) but not against timothy grass extract, an allergen extract with just a few minor allergen homologues in helminths. 116 In more detailed studies, cross-reactivity among allergens and parasite molecules has been well described for tropomyosin, 120,122,129 considered a pan-allergen. 130 Tropomyosins are highly conserved across many species and cross-reactivity is not surprising from the structural point-of-view.…”

Section: -89mentioning

confidence: 99%

Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond

Santiago

Nutman

2016

The American Society of Tropical Medicine and Hygiene

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Abstract. There is much debate about the interaction between helminths and allergic disease. The "Hygiene Hypothesis," a very popular concept among scientists and the lay public, states that infections, especially during childhood, can protect against allergic diseases. Indeed, helminth infections are known to induce regulatory responses in the host that can help the control of inflammation (including allergic inflammation). However, these infections also induce type-2-associated immune responses including helminth-specific IgE that can cross-react against environmental allergens and mediate IgE-driven effector responses. Thus, it is the delicate balance between the parasites' anti-and pro-allergenic effects that define the helminth/allergy interface.The immune system has evolved, in large part, through its interaction with microbes and parasites, an interaction that drives specific or specialized immune responses to deal with the widely varying groups of microorganisms. For example, parasite-derived induction of interleukin (IL)-4, IL-5, and IL-13 coordinate the prototypical responses to metazoan helminth pathogens, 1 whereas viral-and bacterial-specific induction of Type 1 and Type 2 interferons are required for control of these types of infections.

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Structural and immunologic cross-reactivity among filarial and mite tropomyosin: Implications for the hygiene hypothesis

Abstract: Background-The "Hygiene Hypothesis" suggests that parasitic infection modulates host immune responses and decreases atopy. Other data suggest parasitic infections may induce allergic responsiveness.

Cited by 72 publications

References 48 publications

The hygiene hypothesis revisited

The hygiene hypothesis revisited

Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis

Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond

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