Structural and functional characteristics of the tripartite ABC transporter

Okada, Ui; Murakami, Satoshi

doi:10.1099/mic.0.001257

Cited by 11 publications

(4 citation statements)

References 56 publications

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“…The ATP-binding cassette (ABC) transporter differs from other family transporters in that it uses the energy generated by ATP binding and hydrolysis to perform efflux functions ( Okada and Murakami, 2022 ). MsbA was the first ATP-binding cassette transporter to be crystallized and analyzed ( Shilling et al., 2006 ).…”

Section: Resistance Mechanismsmentioning

confidence: 99%

Resistance mechanisms of tigecycline in Acinetobacter baumannii

Sun

Hua

2023

Front. Cell. Infect. Microbiol.

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Acinetobacter baumannii is widely distributed in nature and in hospital settings and is a common pathogen causing various infectious diseases. Currently, the drug resistance rate of A. baumannii has been persistently high, showing a worryingly high resistance rate to various antibiotics commonly used in clinical practice, which greatly limits antibiotic treatment options. Tigecycline and polymyxins show rapid and effective bactericidal activity against CRAB, and they are both widely considered to be the last clinical line of defense against multidrug resistant A. baumannii. This review focuses with interest on the mechanisms of tigecycline resistance in A. baumannii. With the explosive increase in the incidence of tigecycline-resistant A. baumannii, controlling and treating such resistance events has been considered a global challenge. Accordingly, there is a need to systematically investigate the mechanisms of tigecycline resistance in A. baumannii. Currently, the resistance mechanism of A. baumannii to tigecycline is complex and not completely clear. This article reviews the proposed resistance mechanisms of A. baumannii to tigecycline, with a view to providing references for the rational clinical application of tigecycline and the development of new candidate antibiotics.

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Section: Resistance Mechanismsmentioning

confidence: 99%

Resistance mechanisms of tigecycline in Acinetobacter baumannii

Sun

Hua

2023

Front. Cell. Infect. Microbiol.

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“…ABC transporters, the largest gene family of transporter proteins, are present in all organisms [105]. They consist of structurally conserved nucleotide-binding domains and transmembrane domains [106].…”

Section: Swi6 Mediated the Potential Growth And Virulence Of C Fimbri...mentioning

confidence: 99%

The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics

Cong,

Li,

Wang

et al. 2023

Microorganisms

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Ceratocystis fimbriata (C. fimbriata) is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi is located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved in cell wall integrity and virulence in several filamentous pathogenic fungi. However, the specific mechanisms by which Swi6 regulates the growth and pathogenicity of plant pathogenic fungi remain elusive. In this study, the SWI6 deletion mutants and complemented strains of C. fimbriata were generated. Deletion of Swi6 in C. fimbriata resulted in aberrant growth patterns. Pathogenicity assays on sweet potato storage roots revealed a significant decrease in virulence in the mutant. Non-targeted metabolomic analysis using LC-MS identified a total of 692 potential differentially accumulated metabolites (PDAMs) in the ∆Cfswi6 mutant compared to the wild type, and the results of KEGG enrichment analysis demonstrated significant enrichment of PDAMs within various metabolic pathways, including amino acid metabolism, lipid metabolism, nucleotide metabolism, GPI-anchored protein synthesis, and ABC transporter metabolism. These metabolic pathways were believed to play a crucial role in mediating the growth and pathogenicity of C. fimbriata through the regulation of CWI. Firstly, the deletion of the SWI6 gene led to abnormal amino acid and lipid metabolism, potentially exacerbating energy storage imbalance. Secondly, significant enrichment of metabolites related to GPI-anchored protein biosynthesis implied compromised cell wall integrity. Lastly, disruption of ABC transport protein metabolism may hinder intracellular transmembrane transport. Importantly, this study represents the first investigation into the potential regulatory mechanisms of SWI6 in plant filamentous pathogenic fungi from a metabolic perspective. The findings provide novel insights into the role of SWI6 in the growth and virulence of C. fimbriata, highlighting its potential as a target for controlling this pathogen.

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“…Jabbari explores the application of mathematical modelling in understanding microbial efflux [14], emphasizing the utility of non-traditional approaches to study the activity of efflux pumps. Similarly, Athar et al, in their review, highlight the value of computer-based in silico research in studying the structure and function of, as they describe, these beautifully complex machineries [15] Structural and functional aspects of MacAB-TolC ABC transporter are examined by Okada and Murakami [16]. This review provides valuable insights into the mechanisms of substrate recognition, transport, and inhibition of tripartite type VII ABC transporters.…”

mentioning

confidence: 99%

“…Structural and functional aspects of MacAB-TolC ABC transporter are examined by Okada and Murakami [ 16 ]. This review provides valuable insights into the mechanisms of substrate recognition, transport, and inhibition of tripartite type VII ABC transporters.…”

mentioning

confidence: 99%