2001
DOI: 10.1021/ja002500y
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: The measurement of residual dipolar couplings in weakly aligned proteins can potentially provide unique information on their structure and dynamics in the solution state. The challenge is to extract the information of interest from the measurements, which normally reflect a convolution of the structural and dynamic properties. We discuss here a formalism which allows a first order separation of their effects, and thus, a simultaneous extraction of structural and motional parameters from residual dipolar coupli… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

9
318
0
2

Year Published

2002
2002
2023
2023

Publication Types

Select...
5
3

Relationship

0
8

Authors

Journals

citations
Cited by 279 publications
(329 citation statements)
references
References 47 publications
9
318
0
2
Order By: Relevance
“…60 The N60D mutation and the lanthanide substitution do not lead to any significant structural changes in the region flanking the metal coordination site. 52,60,78 PCSs of H N , N nuclei, and RDCs of the H N −N pairs for six paramagnetic lanthanide (CaLn) N (Ca 2 ) C CaMN60D forms in complex with IQ peptide (Ln = Tb, Dy, Ho, Er, Tm, Yb) were measured using 1 H, 15 N HSQC and IPAP spectra, respectively (Figure 1).…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…60 The N60D mutation and the lanthanide substitution do not lead to any significant structural changes in the region flanking the metal coordination site. 52,60,78 PCSs of H N , N nuclei, and RDCs of the H N −N pairs for six paramagnetic lanthanide (CaLn) N (Ca 2 ) C CaMN60D forms in complex with IQ peptide (Ln = Tb, Dy, Ho, Er, Tm, Yb) were measured using 1 H, 15 N HSQC and IPAP spectra, respectively (Figure 1).…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…Residual dipolar couplings (RDCs) can respond to motional averaging over a wide, continuous range of timescales from psec to tens of msec (42,43), an advantage over spin relaxation studies.…”
Section: Fluctuations In the Loops Of Free Ki-fha Are Corroborated Bymentioning
confidence: 99%
“…Deviations of measured RDCs from RDCs predicted from the alignment of the protein structure can result from static or dynamic discrepancy from the protein structural coordinates (42,43). All 1 D NH RDCs were fitted to the whole of each of the ten lowest energy structures from the KI-FHA ensemble (PDB code 1mzk) using PALES (68).…”
Section: Fluctuations In the Loops Of Free Ki-fha Are Corroborated Bymentioning
confidence: 99%
See 1 more Smart Citation
“…Despite their obvious importance, this review does not cover the application of residual dipolar couplings to studies of RNA dynamics, for the sake of retaining focus; the reader is referred to several other excellent papers on this subject. [5][6][7][8] The next section provides Historical Context; section NMR Studies of Nucleic Acids Dynamics introduces common relaxation techniques, and details how they can be applied to nucleic acids. Section Quantitative Analysis of Relaxation Data on RNA and DNA is dedicated to common methods to provide a quantitative analysis, and section Studies of Nucleic Acid Dynamics by 13 C NMR describes some topical application of these methods.…”
Section: Introductionmentioning
confidence: 99%