Structural analysis of the lipopolysaccharide from Vibrio cholerae serotype O22

Cox, Andrew D.; Brisson, Jean‐Robert; Thibault, Pierre; Perry, Malcolm B.

doi:10.1016/s0008-6215(97)00207-3

Cited by 22 publications

(25 citation statements)

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“…1, 2-Aminoethyl phosphate (PEtn) on core OS of one O139 strain (38) and one O22 (37) strain (not reported for the other strains). 2, The O antigen is 133 linked in O22 strains (15,37) and one O139 isolate, whereas a 132 linkage was reported for another O139 strain (14,16). 3, An O-acetyl group in this position was found in O22 strains (15,37).…”

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confidence: 95%

“…3, An O-acetyl group in this position was found in O22 strains (15,37). 4, A second glucose 136 linked to this Glc residue was reported for one O22 strain (15) and one O139 strain (16). Hep, L-glycero-D-manno-heptose; Kdo, 3-deoxy-D-manno-octulosonic acid; Sed f, sedoheptulose (D-altro-heptulose); GlcN, N-acetylglucosamine.…”

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confidence: 98%

“…2, The O antigen is 133 linked in O22 strains (15,37) and one O139 isolate, whereas a 132 linkage was reported for another O139 strain (14,16). 3, An O-acetyl group in this position was found in O22 strains (15,37). 4, A second glucose 136 linked to this Glc residue was reported for one O22 strain (15) and one O139 strain (16).…”

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confidence: 98%

“…1A and B). For the core OS of serogroups O1, O139, and O22 it was found that one D-fructose is linked to the D-glucose residue on HepI (15,16,37,38,68), while in the H11 isolate D-sedoheptulose is found instead at the same position (7,68). Other basic differences between the O1, O139, and O22 core OS and the H11 core OS are the presence of a terminal hepto-FIG.…”

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confidence: 98%

“…(A) LPS core OS backbone proposed for V. cholerae O1, O139, and O22. The representation is based on the structural analysis of two O1, two O139, and two O22 isolates (15,31,37). 1, 2-Aminoethyl phosphate (PEtn) on core OS of one O139 strain (38) and one O22 (37) strain (not reported for the other strains).…”

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Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

et al. 2002

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We identified five different putative wav gene cluster types, which are responsible for the synthesis of the core oligosaccharide (OS) region of Vibrio cholerae lipopolysaccharide. Preliminary evidence that the genes encoded by this cluster are involved in core OS biosynthesis came from analysis of the recently released O1 El Tor V. cholerae genome sequence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of O1 El Tor mutant strains defective in three genes (waaF, waaL, and wavB). Investigations of 38 different V. cholerae strains by Southern blotting, PCR, and sequencing analyses showed that the O1 El Tor wav gene cluster type is prevalent among clinical isolates of different serogroups associated with cholera and environmental O1 strains. In contrast, we found differences in the wav gene contents of 19 unrelated non-O1, non-O139 environmental and human isolates not associated with cholera. These strains contained four new wav gene cluster types that differ from each other in distinct gene loci, providing evidence for horizontal transfer of wav genes and for limited structural diversity of the core OS among V. cholerae isolates. Our results show genetic diversity in the core OS biosynthesis gene cluster and predominance of the type 1 wav gene locus in strains associated with clinical cholera, suggesting that a specific core OS structure could contribute to V. cholerae virulence.Vibrio cholerae is a genetically diverse species that persists in aquatic ecosystems and is often associated with plankton and other aquatic organisms (13). V. cholerae is classified on the basis of biochemical tests and DNA homology studies and is further subdivided into serogroups based on the antigenicity of surface polysaccharides (20). Today more than 193 serogroups are known (72). The ability to cause pandemic cholera is mainly restricted to the nonencapsulated serogroup O1, which is further subdivided mainly into two serotypes (Inaba and Ogawa) and biotypes (classical and El Tor). However, during 1992 and 1993, cholera-like outbreaks in Asia were caused by strains of serogroup O139. Molecular and epidemiological analyses, as well as phage typing, revealed that O139 strains are highly related to the O1 El Tor strains. Hence, it is assumed that the epidemic O139 strains were derived from O1 El Tor strains (for a review see reference 20), differing specifically in the genes encoding for the synthesis of a novel type of cell surface polysaccharide (71). In particular, it was found that the genes encoding the O1 antigen had been replaced by a capsule gene locus carrying the genes involved in the synthesis and transport of the O139 antigen and the O139 capsule (reviewed in reference 64). It was also determined that the structures of the O139 antigen and the O139 capsule were identical (36, 38).There is good evidence that pandemic V. cholerae O1 strains have become adapted to the human intestine by acquisition of virulence factors. Two known factors are cholera toxin (CT), encoded by the filamentous phage CTX⌽, and ...

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confidence: 98%

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confidence: 98%

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confidence: 98%

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Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

et al. 2002

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Diversity and Genetic Basis of Polysaccharide Biosynthesis in Vibrio cholerae

Sozhamannan

Yildiz

2010

Epidemiological and Molecular Aspects on Cholera

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Lipopolysaccharides of Vibrio cholerae

Chatterjee

Chaudhuri

2003

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

153

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Immunological effects of wall lipopolysaccharide (LPS) preparations obtained from Vibrio cholerae Inaba 569B, Ogawa NIH 41 and NAG 4715 strains by the hot phenol-water procedure were examined in mice. Although these LPS lack KDO, which are basic components of the core region of most gram-negative LPS, they still have potencies as B-cell mitogens, adjuvants, immunosuppressants, poly-clonal B-cell activators and phagocytic stimulants for macrophages. The activities of these V. cholerae LPS on murine immune system seemed to be weaker than those of Salmonella typhimurium LT2-LPS. Among these V. cholerae LPS, NAG 4715-LPS showed the strongest mitogenic activity and phagocytic stimulation, while the potencies of this NAG 4715-LPS for the induction of polyclonal B cell activation, adjuvant effects and immunosuppression did not seem to be greater to those of the other LPS. Lipopolysaccharides (LPS) are structural components in the cell walls of gram negative bacteria and these components are easily extractable from many kinds of bacteria, especially Enterobacteriaceae, by physicochemical procedures (14, 23, 24). Similar LPS components could be extracted from Vibrio cholerae organisms (7). However, V. cholerae LPS lack 2-keto-3-deoxyoctonate (KDO) and galactose, which are components of the core region of most gram-negative LPS (10, 14, 23) among their constituents (12, 21). The lipid A in V. cholerae LPS contains unusually large amounts of glycin and ammoniac radicals in comparison with other gram-negative LPS (10). Inaba type vibrio LPS contain considerable amounts of the odd numbered fatty acids, while those of Ogawa and NAG vibrios do not (11). These findings suggest that cholera vibrios may have a fundamentally different LPS structure from that of Salmonella and other gram-negative bacteria, and, furthermore, structural differences of lipid A components in LPS may exist among V. cholerae strains. Recently, much attention has been focused on the effects of LPS on immune phenomena (1, 2, 4, 15, 22). Almost all of the experiments to examine the immuno-logical properties of LPS have been performed using LPS prepared from gram-negative rods, but not from vibrios. Therefore, it may be worthwhile to examine whether or not the structurally different V. cholerae LPS are capable of stimulating immune 611

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Structural analysis of the lipopolysaccharide from Vibrio cholerae serotype O22

Cited by 22 publications

References 40 publications

Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

Diversity and Genetic Basis of Polysaccharide Biosynthesis in Vibrio cholerae

Lipopolysaccharides of Vibrio cholerae

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