Strongyloides venezuelensis: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis

Machado, Eleuza Rodrigues; Faccioli, Lúcia Helena; Costa-Cruz, Júlia Maria; Lourenço, Elaine; Roque-Barreira, Maria Cristina; Gonçalves-Pires, Maria do Rosário de Fátima; Ueta, Marlene Tiduko

doi:10.1016/j.exppara.2007.12.008

Cited by 30 publications

(26 citation statements)

References 27 publications

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“…In the present study using another heterologous antigen and its D phase were achieved better positivity indexes supported by an elaborated statistical analyses, providing evidence that saliva of patients with strongyloidiasis contains antibodies to S. stercoralis that can be detected by total saline extract and D phase of S. venezuelensis antigens. The findings of Machado et al [36] support the use of S. venezuelensis antigen. They isolated different strains of S. venezuelensis from diverse wild rodents and kept these strains in lab rats observing that there was no influence on the antigenic profile of L3.…”

Section: Discussionsupporting

confidence: 54%

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE(*)) and its detergent phase (D) extracted with Triton X-114. Saliva and serum paired samples were obtained from: 25 patients with confirmed strongyloidiasis; 25 patients with other parasitoses and 20 from apparently healthy individuals. Sensitivity, specificity, diagnostic efficiency, positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction. Using D phase sensitivity and specificity to detect IgA in saliva were 76.0% and 88.9% and in serum 80.0% and 86.7%, respectively. To detect IgG, D phase showed sensitivity and specificity of 88.0% and 88.9% in saliva and 88.0% and 84.4% in serum, respectively. D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis.

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Section: Discussionsupporting

confidence: 54%

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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“…Alkaline extract of larvae (L3‐ALK) or adult (F‐ALK) was prepared in accordance with Machado et al . . In addition, deoxycholic alkaline extract of larvae (L3‐ALK‐DOC) and adults (F‐ALK‐DOC) was prepared in accordance with Herbert et al .…”

Section: Methodsmentioning

confidence: 99%

“…Larvae or adults were suspended in PBS and 0.15 M sodium hydroxide with protease inhibitors as above. Alkaline extract of larvae (L3-ALK) or adult (F-ALK) was prepared in accordance with Machado et al (16). In addition, deoxycholic alkaline extract of larvae (L3-ALK-DOC) and adults (F-ALK-DOC) was prepared in accordance with Herbert et al (15).…”

Section: Parasite and Antigen Preparationsmentioning

confidence: 99%

Apoptotic mechanisms are involved in the death of Strongyloides venezuelensis after triggering of nitric oxide

Ruano

López‐Abán

Gajate

et al. 2012

Parasite Immunology

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Despite progress in understanding the role of nitric oxide (NO) in the pathogenesis of helminth infections, the role in strongyloidosis is unknown. Firstly, we studied the production of NO in mice infected with Strongyloides venezuelensis as well as in macrophage cultures stimulated with parasite antigens. Somatic larvae 3 (L3) and excretory-secretory female antigens stimulate specific NO production measured by Griess reaction and expression of inducible NO synthase by RT-PCR and quantitative PCR. Moreover, mice infected with S. venezuelensis produce NO in migration stages. Secondly, we analysed the effect of NO production on L3 and females of S. venezuelensis using NO donors such as diethylenetriamine and 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene. Parasites died after NO donor treatment in a dose-dependent manner. Finally, apoptotic mechanisms are involved in the death of S. venezuelensis larvae.

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“…This nematode has also been used to standardise new immunological techniques to optimise human strongyloidiasis diagnosis (Machado et al 2008(Machado et al , 2009.…”

mentioning

confidence: 99%

Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats

Marra

Chiuso-Minicucci

Machado

et al. 2010

Mem. Inst. Oswaldo Cruz

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Strongyloides venezuelensis: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis

Cited by 30 publications

References 27 publications

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Apoptotic mechanisms are involved in the death of Strongyloides venezuelensis after triggering of nitric oxide

Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats

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