Stromal vascular fraction cells plus sustained release VEGF/Ang‐1‐PLGA microspheres improve fat graft survival in mice

He, Yucang; Yu, Xiaofang; Chen, Zhuojie; Li, Liqun

doi:10.1002/jcp.27368

Cited by 16 publications

(8 citation statements)

References 35 publications

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“…Previous research found that stromal vascular fraction cells (SVFs) could improve fat graft survival in mice 28 . As an important component of SVFs, ADSCs appear to be pure and effective for co‐transplantation with fat grafts.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the key factors in the differentiation of ADSCs into pericytes, the interaction of HUVECs and Previous research found that stromal vascular fraction cells (SVFs) could improve fat graft survival in mice. 28 As an important component of SVFs, ADSCs appear to be pure and effective for co-transplantation with fat grafts. The present study developed a new aspect of promoting vascularization in fat grafting by regulating miR-24-3p in ADSCs.…”

Section: Discussionmentioning

confidence: 99%

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MiR‐24‐3p regulates the differentiation of adipose‐derived stem cells toward pericytes and promotes fat grafting vascularization

Cai

Wei

et al. 2023

The FASEB Journal

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Adipose‐derived stem cells (ADSCs) enhance fat graft survival by promoting neovascularization. The mechanism that promotes ADSCs differentiation toward pericytes was not known. We treated ADSCs with conditional medium (CM) from endothelial cells (ECs) or human recombinant transforming growth factor β (TGF‐β) to induce differentiation into pericytes. Pericytes markers, including platelet‐derived growth factor receptor β (PDGFRβ), alpha‐smooth muscle actin (α‐SMA), and desmin, were examined. Pericytes differentiation markers, migration, and their association with ECs were examined in ADSCs transfected with miR‐24‐3p mimics and inhibitors. Bioinformatics target prediction platforms and luciferase assays were used to investigate whether PDGFRβ was directly targeted by miR‐24‐3p. In vivo, fat mixed with ADSCs transfected with miR‐24‐3p mimics or inhibitors was implanted subcutaneously on the lower back region of nude mice. Fat grafts were harvested and analyzed at 2, 4, 6, and 8 weeks. Results showed that endogenous TGF‐β derived from CM from EC or human recombinant TGF‐β promoted migration, association with ECs, and induced expression of pericyte markers (PDGFRβ, α‐SMA, Desmin) in ADSCs. MiR‐24‐3p directly targeted PDGFRβ in ADSCs by lucifer reporter assays. Inhibition of miR‐24‐3p promoted pericytes differentiation, migration, and association with ECs in ADSCs. Inhibition of miR‐24‐3p in ADSCs promoted survival, integrity, adipocyte viability, vascularization, pericytes association with ECs, and reduced fibrosis, whereas overexpression of miR‐24‐3p in ADSCs yielded the opposite results. Collectively, TGF‐β released by ECs induced ADSCs differentiation toward pericytes through miR‐24‐3p. Downregulation of miR‐24‐3p in ADSCs induced survival, integrity, adipocyte viability, vascularization, pericytes association with ECs, and reduced fibrosis after fat grafting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MiR‐24‐3p regulates the differentiation of adipose‐derived stem cells toward pericytes and promotes fat grafting vascularization

Cai

Wei

et al. 2023

The FASEB Journal

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“…The slow vascular regeneration process suppressed the lipolysis and β-oxidation of fatty acids, and resulted in the formation of oil cysts. Conversely, decreased oil cysts were observed in fat grafts assisted by stromal vascular fraction cells or vascular endothelial growth factor (VEGF) ( Lu et al, 2009 ; He et al, 2019 ). Simultaneously, macrophages act as major immune cells in promoting angiogenesis and vascular regeneration ( Godwin et al, 2013 ; Godwin et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

A Short-Term High-Fat Diet Improved the Survival of Fat Grafts in Mice by Promoting Macrophage Infiltration and Angiogenesis

Niu

Lai

Chen

et al. 2022

Front. Cell Dev. Biol.

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Background: Adipose tissue is an ideal filler material that is widely used for soft tissue defects. But the low survival rate and complications associated with such grafts pose a serious challenge, which limits their clinical application. Adipose tissue is a metabolic diet-responsive tissue; however, the influence of diets on fat grafting remains ambiguous.Methods: We extracted inguinal fat pads from C57/BL6 male mice, and transplanted them into the dorsal region of recipient mice (0.3 ml). Post-fat-grafting, mice (n = 54) were randomized into three groups, namely normal diet (ND), high carbohydrate diet (HC), and high-fat diet (HF). Structural changes were assessed by histological staining. Lipolysis activity and vascular regeneration of grafts on day 30 were analyzed using real-time polymerase chain reaction, immunofluorescence, and western blotting.Results: The grafts of mice on HC and HF diets exhibited significantly fewer oil cysts and larger volume retention (0.18 ± 0.01, 0.21 ± 0.01, and 0.25 ± 0.01 ml, for ND, HC, and HF group, respectively, p < 0.05) on day 90. In comparison, grafts for the mice belonging to the HF groups exhibited higher expression of lipolysis-related genes, including adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and carnitine palmitoyltransferase 1 (CPT1), on day 30. Furthermore, increased infiltration of macrophages (F4/80+) and the higher expression of angiogenesis genes were reported in the HF groups.Conclusion: Altogether, the administration of short-term HF diet remarkably enhanced angiogenesis and improved the quality of fat grafts, which was characterized by fewer oil cysts and higher long-term volume retention. The possible mechanisms may be due to the increased macrophage infiltration, and the promoted angiogenesis in HF grafts.

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“…Previous studies have attempted to solve the abovementioned problems by improving the blood supply or increasing adipose stem cells. For example, stromal vascular components (SVFs) [21], cell-assisted fat transplantation [22] and cytokines have been used [23]. Although substantial progress has been made, many complications persist, and the cumbersome operation is also a hindrance.…”

Section: Discussionmentioning

confidence: 99%

Salvianolic Acid B Improves the Effect of Fat Grafts by Inhibiting the NF-kB Signalling Pathway in Macrophages

Sun¹,

Ho²,

Gao³

et al. 2021

Preprint

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Background Autologous fat grafting (AFG), although an appealing approach to repair soft tissue defects, has various complications. Excessive inflammation at the transplant site is one of the main reasons for the poor effect of fat transplantation and occurrence of complications. Our previous study proved that Salvia miltiorrhiza can enhance fat graft survival. Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza and has anti-inflammatory effects on other diseases. Therefore, we hypothesized that salvianolic acid B could improve the effect of fat grafts by inhibiting inflammation. Methods In vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2, 4 and 12 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the proliferative and anti-inflammatory activities of salvianolic acid B were analyzed in cultured RAW264.7 cells to detect the mechanism by which salvianolic acid B affects graft survival by inhibiting inflammation. Results In vivo, the degree of adipose tissue fibrosis and inflammatory cell infiltration in the salvianolic acid B treatment group was lower, and the infiltration of M1 macrophages in fat grafts was also less than that in the control group. In vitro, salvianolic acid B inhibited the proliferation and activation of inflammatory pathways in RAW264.7 cells. Conclusions This study demonstrates the use of salvianolic acid B as a possible treatment to improve the effect of fat transplantation.

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Stromal vascular fraction cells plus sustained release VEGF/Ang‐1‐PLGA microspheres improve fat graft survival in mice

Abstract: Autologous fat transplantation is increasingly applied in plastic and reconstructive surgery.

Cited by 16 publications

References 35 publications

MiR‐24‐3p regulates the differentiation of adipose‐derived stem cells toward pericytes and promotes fat grafting vascularization

MiR‐24‐3p regulates the differentiation of adipose‐derived stem cells toward pericytes and promotes fat grafting vascularization

A Short-Term High-Fat Diet Improved the Survival of Fat Grafts in Mice by Promoting Macrophage Infiltration and Angiogenesis

Salvianolic Acid B Improves the Effect of Fat Grafts by Inhibiting the NF-kB Signalling Pathway in Macrophages

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