Stromal cell-induced immune regulation in a transplantable lymphoid-like cell constructs

Kaminer-Israeli, Yael; Shapiro, Jenny; Cohen, Smadar; Monsonego, Alon

doi:10.1016/j.biomaterials.2010.08.070

Cited by 6 publications

(5 citation statements)

References 51 publications

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“…Several studies have investigated the combination of MSCs and immune cells when coseeded in 3D environments. 52 , 53 With such experimental designs, it is not possible to isolate the secretions of MSCs from those of immune cells. The included studies all had 2D controls seeded in the same cell density as in the 3D structures.…”

Section: Limitationsmentioning

confidence: 99%

Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture

Follin

Juhl

CohenSmadar

et al. 2016

Tissue Engineering Part B: Reviews

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Mesenchymal stromal/stem cells (MSCs) have been investigated extensively through the past years, proving to have great clinical therapeutic potential. In vitro cultivation of MSCs in three-dimensional (3D) culture systems, such as scaffolds, hydrogels, or spheroids, have recently gained attention for tissue engineering applications. Studies on MSC spheroids demonstrated that such cultivation increased the paracrine immunomodulatory potential of the MSCs, accompanied by phenotypic alterations. In this review, we gather results from recent experimental studies on the immunomodulatory abilities of MSCs when cultured as spheroids or in biomaterials like scaffolds or hydrogels compared to regular two-dimensional (2D) culture and show that alterations occurring to MSCs in spheroids also occur in MSCs in biomaterials. We provide a brief description of known mechanisms of MSC immunomodulatory capacity and how they are altered in the two 3D culture systems, together with phenotypic cellular changes. Based on the present knowledge, we highlight vital areas in need of further investigation. The impact of 3D environments on immunomodulation has great potential for tissue engineering and cellular therapy, and this is the first review to gather this knowledge with a comparison across different 3D environments.

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Section: Limitationsmentioning

confidence: 99%

Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture

Follin

Juhl

CohenSmadar

et al. 2016

Tissue Engineering Part B: Reviews

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“…Freeze-dried hydrogels were sterilized under ultraviolet irradiation in a biological cabinet for 1 h . Hydrogels were then placed and fixed at one end in a four-well plate equipped with carbon electrodes.…”

Section: Methodsmentioning

confidence: 99%

“…Cell-Seeded Hydrogels. Freeze-dried hydrogels were sterilized under ultraviolet irradiation in a biological cabinet for 1 h. 25 Hydrogels were then placed and fixed at one end in a four-well plate equipped with carbon electrodes. Primary porcine smooth muscle cells (5 × 10 5 pSMCs) suspended in 100 μL of culture medium (SmBM) were added to the surface of the freeze-dried hydrogel strips (15 mm × 4 mm × 1 mm).…”

Section: ■ Introductionmentioning

confidence: 99%

Electrosensitive Polyacrylic Acid/Fibrin Hydrogel Facilitates Cell Seeding and Alignment

Rahimi¹,

Molin²,

Cleij

et al. 2012

Biomacromolecules

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Three-dimensional cell culture and conditioning is an effective means to guide cell distribution and patterning for tissue engineered constructs such as vascular grafts. Polyacrylic acid is known as an electroresponsive polymer, capable of transforming environmental stimuli like electrical energy to mechanical forces. In this study, we developed an electrosensitive and biocompatible hydrogel-based smart device composed of acrylic acid and fibrin as a tissue engineered construct to mechanically stimulate cells. Structural properties of the hydrogel were assessed by FTIR-ATR, scanning electron microscopy, prosimetry, and swelling measurement. Distribution and alignment of porcine smooth muscle cells (pSMCs) seeded on the surface of lyophilized hydrogels were evaluated and quantified by two-photon laser scanning microscopy. Smooth muscle cell tissue constructs exposed to 2 h of pulsatile electrical stimulation showed significantly enhanced cell penetration and alignment due to dynamic changes produced by alternative swelling and deswelling, in comparison with static samples. On the basis of the results, this hydrogel under electrical stimulation works as a mechanical pump, which can direct SMC alignment and facilitate infiltration and distribution of cells throughout the structure.

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“…We demonstrated the fabrication of a stromal cell-based construct, which serves as a lymphoid-like organ with immune regulatory characteristics [57]. In this system, stromal cells (terminally differentiated progeny of MSCs) were co-seeded with dendritic cells (DCs) in a macro-porous alginate scaffold containing the encephalitogenic myelin-derived peptide, proteolipid protein (PLP).…”

Section: Immunomodulationmentioning

confidence: 99%

“…In vitro, we showed that while the migration of pathogenic PLP-specific T cells to the construct cultivated with or without stromal cells does not differ, their activation and proliferation are significantly suppressed in the presence of stromal cells. Upon in vivo transplantation, under the kidney capsule of mice, the pathogenic activation and proliferation of T cells which were drawn into the construct, were suppressed in the co-seeded constructs [57]. This system thus serves as a lymphoid-like organ with regulatory characteristics, which can be applied for local tolerance induction, for application in cell transplantations as well as autoimmune diseases.…”

Section: Immunomodulationmentioning

confidence: 99%

Bioengineering Alginate for Regenerative Medicine Applications

Ruvinov

Cohen

2016

Biomaterials From Nature for Advanced Devices and Therapies

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Stromal cell-induced immune regulation in a transplantable lymphoid-like cell constructs

Cited by 6 publications

References 51 publications

Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture

Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture

Electrosensitive Polyacrylic Acid/Fibrin Hydrogel Facilitates Cell Seeding and Alignment

Bioengineering Alginate for Regenerative Medicine Applications

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