Strobilanthes crispus inhibits migration, invasion and metastasis in breast cancer

Baraya, Yusha'u Shu'aibu; Wong, Kah Keng; Yaacob, Nik Soriani

doi:10.1016/j.jep.2018.12.041

Cited by 17 publications

(16 citation statements)

References 35 publications

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“…Wound closure through cell migration was measured at 0, 24 and 48 h under a microscope. The cells migration towards the wound scratches was expressed as migrated cells percentage and calculated as [ 90 ]: Migrated cells percentage = [(At = 0 h − At = Δh)/At = 0 h] × 100 where,…”

Section: Methodsmentioning

confidence: 99%

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Deciphering the Molecular Mechanism Responsible for Efficiently Inhibiting Metastasis of Human Non-Small Cell Lung and Colorectal Cancer Cells Targeting the Matrix Metalloproteinases by Selaginella repanda

Adnan

Siddiqui

Hamadou

et al. 2021

Plants

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Selaginella species are known to have antimicrobial, antioxidant, anti-inflammatory, anti-diabetic as well as anticancer effects. However, no study has examined the cytotoxic and anti-metastatic efficacy of Selaginella repanda (S. repanda) to date. Therefore, this study aimed to evaluate the potential anti-metastatic properties of ethanol crude extract of S. repanda in human non-small-cell lung (A-549) and colorectal cancer (HCT-116) cells with possible mechanisms. Effect of S. repanda crude extract on the growth, adhesion, migration and invasion of the A-549 and HCT-116 were investigated. We demonstrated that S. repanda crude extract inhibited cell growth of metastatic cells in a dose and time dependent manner. Incubation of A-549 and HCT-116 cells with 100–500 µg/mL of S. repanda crude extract significantly inhibited cell adhesion to gelatin coated surface. In the migration and invasion assay, S. repanda crude extract also significantly inhibited cellular migration and invasion in both A-549 and HCT-116 cells. Moreover, reverse transcription-polymerase chain reaction, and real-time PCR (RT-PCR) analysis revealed that the activity and mRNA level of matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-2 (MMP-2) and membrane type 1-matrix metalloproteinase (MT1-MMP) were inhibited. While the activity of tissue inhibitor matrix metalloproteinase 1 (TIMP-1); an inhibitor of MMPs was stimulated by S. repanda crude extract in a concentration-dependent manner. Therefore, the present study not only indicated the inhibition of motility and invasion of malignant cells by S. repanda, but also revealed that such effects were likely associated with the decrease in MMP-2/-9 expression of both A-549 and HCT-116 cells. This further suggests that S. repanda could be used as a potential source of anti-metastasis agent in pharmaceutical development for cancer therapy.

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Section: Methodsmentioning

confidence: 99%

“…Plates were then incubated for 48 h at 37 • C. Wound closure through cell migration was measured at 0, 24 and 48 h under a microscope. The cells migration towards the wound scratches was expressed as migrated cells percentage and calculated as [90]:…”

Section: Wound Closure Assaymentioning

confidence: 99%

Deciphering the Molecular Mechanism Responsible for Efficiently Inhibiting Metastasis of Human Non-Small Cell Lung and Colorectal Cancer Cells Targeting the Matrix Metalloproteinases by Selaginella repanda

Adnan

Siddiqui

Hamadou

et al. 2021

Plants

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“…Baraya et al studied the anti-migration, anti-invasion and anti-metastasis effects of S. crispus leaves in MDA-MB-231 breast cancer cells by using a standardized sub-fraction called F3 [58]. F3 contained a variety of constituents, including β-sitosterol, stigmasterol, campesterol, lutein, pheophytin a, 131-hydroxy-132-oxo-pheophytin a, and 132-hydroxy-pheophytin a.…”

Section: Panax Ginseng (Asian Ginseng)mentioning

confidence: 99%

“…There are numerous studies done by examining the cytotoxic effects of various S. crispus extracts against normal cell lines [56,58,63]. Normal breast epithelial cell line (MCF10A) and Chang liver (normal) cell line were used in some studies and the results showed that S. crispus extracts were not cytotoxic against normal cells.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Anticancer Properties of Strobilanthes crispus: A Review

et al. 2021

Processes

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Cancer is a major cause of death worldwide, as exemplified by millions of cancer diagnoses every year. The use of chemotherapy in treating cancer has many disadvantages which include recurrence of cancer, associated with drug resistance, and severe side effects that are harmful to the patients. A better source of anticancer drugs can come from nature. Strobilanthes crispus (S. crispus) is a herbal medicinal plant that is indigenous in Madagascar and the Malay Archipelago. The plant possesses high vitamin and mineral content as well as phytochemicals—like phenols, catechins, tannins, and flavonoids—that are known to have therapeutic effects. Numerous preclinical studies have reported very versatile pharmacological effects of this plant, such as anticancer, antimicrobial, antioxidant, anti-angiogenesis, anti-diabetes, anti-ulcerogenic, and wound healing. Herein, this paper reviews the anticancer properties of S. crispus, providing information for future research and further exploration.

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“…Recently, F3 was shown to inhibit migration and invasion of human MDA-MB-231 and murine 4T1 breast cancer cell lines as well as to suppress cancer progression in 4T1 mammary tumor model by disrupting the metastatic process. The anti-metastatic effect was accompanied by restoration of E-cadherin expression and downregulation of epithelial-mesenchymal transition (EMT) proteins [ 23 ]. These findings suggest the potential of F3 to help improve patient survival rate through prevention of metastasis.…”

Section: Introductionmentioning

confidence: 99%

Strobilanthes crispus elicits anti-tumor immunogenicity in in vitro and in vivo metastatic breast carcinoma

et al. 2022

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Plant-based anticancer agents have the potential to stimulate the immune system to act against cancer cells. A standardized bioactive subfraction of the Malaysian herb, Strobilanthes crispus (L.) Blume (S. crispus) termed F3, demonstrates strong anticancer effects in both in vitro and in vivo models. The anticancer effects might be attributable to its immunomodulatory properties as S. crispus has been traditionally used to enhance the immune system. The current study examined whether F3 could stimulate anti-tumorigenic immunogenicity against 4T1 cells in vitro and in 4T1 cell-induced mammary carcinoma mouse model. We observed that F3 induced significant increase in MHC class I and class II molecules. CD4+, CD8+ and IL-2+ (p<0.05 for all) cells infiltration was also significantly increased in the breast tumor microenvironment of F3-treated mice compared with the tumors of untreated mice. The number of CD68+ macrophages was significantly lower in F3-treated mice. We conclude that the antitumor and antimetastatic effects of S. crispus involve strong infiltration of T cells in breast cancer potentially through increased tumor antigen presentation via MHC proteins, as well as reduction of infiltrating tumor-associated macrophages.

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Strobilanthes crispus inhibits migration, invasion and metastasis in breast cancer

Cited by 17 publications

References 35 publications

Deciphering the Molecular Mechanism Responsible for Efficiently Inhibiting Metastasis of Human Non-Small Cell Lung and Colorectal Cancer Cells Targeting the Matrix Metalloproteinases by Selaginella repanda

Deciphering the Molecular Mechanism Responsible for Efficiently Inhibiting Metastasis of Human Non-Small Cell Lung and Colorectal Cancer Cells Targeting the Matrix Metalloproteinases by Selaginella repanda

Anticancer Properties of Strobilanthes crispus: A Review

Strobilanthes crispus elicits anti-tumor immunogenicity in in vitro and in vivo metastatic breast carcinoma

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