Streulichtphotometer zur kontinuierlichen Bestimmung der Biomassekonzentration in Fermentationsmedien

Abstract

After a short review of methods for the determination of the biomass concentration in fermentation broths the construction and working principle of a newly developed retroreflective photometer are described. The main advantages of the instrument are a wide range from 0.1 to 200 g biomass/l the sterilizability, the self‐cleaning of the cuvette‐window, a favourable design and a little maintenance. The device may be used directly at a fermenter to get a continuous singal or for measurement of single samples in the laboratory. The calibration curves are nearly linear in a wide range.

Noninvasive online biomass detector system for cultivation in shake flasks

“…Due to their proportionality to each other, OD 600 can be used as a substitute for CDW. This is already established in previous publications which deal with scattered light sensors [25]. Figure 5A clearly shows that there is a linear correlation between OD 600 and CDW in the measurement range of the presented microorganisms.…”