Stress protein synthesis induced by cadmium-cysteine in rat kidney

Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, and CdCl 2

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21 (31). The only in vivo data in the kidney detailing hsp 70 expression was the previously detailed study in the rat kidney that examined hsc 70 expression after administration of Cd2+ as both the CdCl2 salt and as the Cd-Cys complex (22). In this study it was also demonstrated that acute exposure of rats to iv-administered CdCl2 had no effect on the expression of renal hsp 70, whereas identical exposure to the Cd-Cys complex resulted in a modest (2-fold) elevation of hsp 70.…”

“…). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd>(22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, and CdCl 2

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21 (31). The only in vivo data in the kidney detailing hsp 70 expression was the previously detailed study in the rat kidney that examined hsc 70 expression after administration of Cd2+ as both the CdCl2 salt and as the Cd-Cys complex (22). In this study it was also demonstrated that acute exposure of rats to iv-administered CdCl2 had no effect on the expression of renal hsp 70, whereas identical exposure to the Cd-Cys complex resulted in a modest (2-fold) elevation of hsp 70.…”

“…). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd>(22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

Expression of the constitutive and inducible forms of heat shock protein 70 in human proximal tubule cells exposed to heat, sodium arsenite, and CdCl(2).

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd> (22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

The comparison of the protective effect of zinc and selenite ions against cadmium induced changes of protein synthesis in mice organs

“…In our experiments, protein synthesizing system in skeletal muscle did not respond to Cd2+ exposure after a 6-week period. According to other authors, toxicity of Cd causes activation of protein (MT, heatshock proteins) and GSH synthesis, which are adaptive mechanisms of organisms [45,47,48]. In conformity with Nordberg et al [49] there is a relation between MT gene ex pression levels and the oxidative stress status of the cells.…”

Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, and CdCl 2

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21 (31). The only in vivo data in the kidney detailing hsp 70 expression was the previously detailed study in the rat kidney that examined hsc 70 expression after administration of Cd2+ as both the CdCl2 salt and as the Cd-Cys complex (22). In this study it was also demonstrated that acute exposure of rats to iv-administered CdCl2 had no effect on the expression of renal hsp 70, whereas identical exposure to the Cd-Cys complex resulted in a modest (2-fold) elevation of hsp 70.…”

“…). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd>(22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

Expression of the constitutive and inducible forms of heat shock protein 70 in human proximal tubule cells exposed to heat, sodium arsenite, and CdCl(2).

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd> (22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

The comparison of the protective effect of zinc and selenite ions against cadmium induced changes of protein synthesis in mice organs

“…In our experiments, protein synthesizing system in skeletal muscle did not respond to Cd2+ exposure after a 6-week period. According to other authors, toxicity of Cd causes activation of protein (MT, heatshock proteins) and GSH synthesis, which are adaptive mechanisms of organisms [45,47,48]. In conformity with Nordberg et al [49] there is a relation between MT gene ex pression levels and the oxidative stress status of the cells.…”

Expression of the Constitutive and Inducible Forms of Heat Shock Protein 70 in Human Proximal Tubule Cells Exposed to Heat, Sodium Arsenite, and CdCl 2

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21 (31). The only in vivo data in the kidney detailing hsp 70 expression was the previously detailed study in the rat kidney that examined hsc 70 expression after administration of Cd2+ as both the CdCl2 salt and as the Cd-Cys complex (22). In this study it was also demonstrated that acute exposure of rats to iv-administered CdCl2 had no effect on the expression of renal hsp 70, whereas identical exposure to the Cd-Cys complex resulted in a modest (2-fold) elevation of hsp 70.…”

“…). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd>(22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

Expression of the constitutive and inducible forms of heat shock protein 70 in human proximal tubule cells exposed to heat, sodium arsenite, and CdCl(2).

“…Furthermore, these findings are in agreement with the initial study that described hsc 70 as a constitutive protein with a high level of expression, attaining a level that can comprise 1% of total cell protein under nonstressed conditions (21). While the majority of studies on the heat shock response are cell culture based, one study has examined hsc 70 expression in the rat kidney after administration of Cd> (22). In agreement with the present findings, this study demonstrated that Cd2+, when administered intravenously (iv) as CdCl2 (2 mg/kg), had no effect on the level of expression of hsc 70 protein in the kidney 4 hr after exposure.…”

The comparison of the protective effect of zinc and selenite ions against cadmium induced changes of protein synthesis in mice organs

“…In our experiments, protein synthesizing system in skeletal muscle did not respond to Cd2+ exposure after a 6-week period. According to other authors, toxicity of Cd causes activation of protein (MT, heatshock proteins) and GSH synthesis, which are adaptive mechanisms of organisms [45,47,48]. In conformity with Nordberg et al [49] there is a relation between MT gene ex pression levels and the oxidative stress status of the cells.…”