Streptozotocin- induced changes of aquaporin 1 and 4, oxidative stress and autophagy in submandibular and parotid salivary glands and the possible ameliorative effect of intermittent fasting on these changes: immunohistochemical and molecular study in rats.

Taha, Reham; Alghamdi, Mansour A.; Hanan, Alshehri; Qahtani, Eman Ali Al; Al-Khater, Khulood M.; Aldahhan, Rashid A; Nashar, Eman Mohamad El

doi:10.22541/au.167226841.12359525/v1

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“…Furthermore, IF was shown to be helpful in treating certain conditions through the regulation of AQPs expression in different tissues, e.g., brain, peritoneum, salivary glands and liver ( Zhang et al, 2017 ; Taha et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Potential role for vitamin D vs. intermittent fasting in controlling aquaporin-1 and aquaporin-3 expression in HFD-induced urinary bladder alterations in rats

Hassan,

El-Gamal,

Hamed

et al. 2024

Front. Mol. Biosci.

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Background: High-fat diet-induced obesity is linked to suppression of aquaporins (AQPs) expression in different tissues. Both vitamin D and intermittent fasting were identified to enhance AQPs expression. In the urinary bladder, AQP-1 and AQP-3 mRNA transcripts were identified. Vitamin D has an impact on a variety of genes that encode proteins that control cell proliferation, differentiation, and death.Aim: To assess potential benefits of vitamin D and intermittent fasting (IF) and to explore alterations to the urinary bladder triggered by high-fat diet (HFD) in a rat model of obesity.Methods: Each of the 4 groups contained six adult male albino rats; control: a standard rodent chew for 12 weeks, HFD: HFD and fructose were administered orally via gastric gavage for 12 weeks, and vitamin D: HFD and fructose were administered orally for 8 weeks, then 4 weeks of intraperitoneal injection of vitamin D (5 microns/Kg/2 days) and IF group: Received intraperitoneal injections of vitamin D (5 microns/Kg/2 days) for 4 weeks after consumption of HFD and fructose orally for 8 weeks. The serum lipid profile was conducted at end of the experiment. In the bladder homogenates, the levels of oxidative stress indicators were assessed. Quantitative real-time PCR was performed on recently collected bladder samples. AQP-1 and AQP-3 immunohistochemistry was done.Results: When compared to the HFD group, the vitamin D and IF groups both demonstrated a substantial improvement in histopathological, immunohistochemical, biochemical, and molecular markers.Conclusion: In all examined parameters, IF exceeded vitamin D as a preventive factor for the urinary bladder deterioration.

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Section: Introductionmentioning

confidence: 99%