Streptomycete origin of chromosomal replication with two putative unwinding elements

AdpA Positively Regulates Morphological Differentiation and Chloramphenicol Biosynthesis in Streptomyces venezuelae

“…In silico analyses indicated that the organization of putative AdpA boxes within the oriC regions of all three species differs significantly even though these oriCs share a relatively high level of sequence similarity (above 77% identity) and nearly identical arrangement of the binding sites for the replication initiator, DnaA (Fig. S5B and reference 80 ). In S. coelicolor , AdpA competes with DnaA for binding to oriC and thus presumably hinders DnaA-dependent DNA unwinding.…”

Identification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replication

“…However, the importance of HU for replication initiation has only been clearly demonstrated for E. coli ( Krause et al, 1997 ; Chodavarapu et al, 2008 ). Several studies have shown HU independent origin unwinding even in gram-negative bacteria ( Donczew et al, 2012 ; Makowski et al, 2016 ; Jaworski et al, 2018 ; Plachetka et al, 2019 ), suggesting that HU-dependence of origin unwinding may be limited to a narrow phylogenetic group. C. difficile encodes a homolog of HU, HupA ( Oliveira Paiva et al, 2019 ) but whether this protein plays a role in DNA replication initiation remains to be established.…”