Streptomyces sp. M54: an actinobacteria associated with a neotropical social wasp with high potential for antibiotic production

Matarrita-Carranza, Bernal; Murillo‐Cruz, Catalina; Avendaño, Roberto; Ríos, María Isabel Espín; Chavarría, Max; Gómez-Calvo, María Luisa; Tamayo‐Castillo, Giselle; Araya, Juan J.; Pinto‐Tomás, Adrián A.

doi:10.1007/s10482-021-01520-y

Cited by 12 publications

(16 citation statements)

References 86 publications

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“…Probably due to this special configuration, antiSMASH was not able to predict the putative cluster responsible for their biosynthesis. As described by Matarrita-Carranza and co-workers [55], the nonactin BGC could only be detected using the antiSMASH loose strictness parameter. If we ran an antiSMASH analysis set with this new configuration, we were able to find a BGC classified as fatty acid that retrieved an 100% similarity to the macrotetrolide BGC (BGC0000243) and 57% to the nonactin BGC (BGC0000252).…”

Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 79%

“…If we ran an antiSMASH analysis set with this new configuration, we were able to find a BGC classified as fatty acid that retrieved an 100% similarity to the macrotetrolide BGC (BGC0000243) and 57% to the nonactin BGC (BGC0000252). As nonactins are the parent compounds of macrotetrolides [56] and both clusters were usually found together [55], we could assign this BGC as the responsible for the nonactin production detected by dereplication in CS065a culture samples. Finally, mining through the CS090a genome sequence we were only able to find one NRPS type BGC with moderate similarity (56%) to the valinomycin/montanastatin BGC (BGC0001846; [57]) and to the valinomycin BGC (BGC0000453; [58]).…”

Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 87%

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A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

et al. 2021

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The rapid emergence of bacterial resistance to antibiotics has urged the need to find novel bioactive compounds against resistant microorganisms. For that purpose, different strategies are being followed, one of them being exploring secondary metabolite production in microorganisms from uncommon sources. In this work, we have analyzed the genome of 12 Streptomyces sp. strains of the CS collection isolated from the surface of leaf-cutting ants of the Attini tribe and compared them to four Streptomyces model species and Pseudonocardia sp. Ae150A_Ps1, which shares the ecological niche with those of the CS collection. We used a combination of phylogenetics, bioinformatics and dereplication analysis to study the biosynthetic potential of our strains. 51.5% of the biosynthetic gene clusters (BGCs) predicted by antiSMASH were unknown and over half of them were strain-specific, making this strain collection an interesting source of putative novel compounds.

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Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 79%

Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 87%

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

et al. 2021

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“…In an attempt to elucidate the role of the members of the microbiome in both sloth species and considering that many of these microorganisms belong to the phylum Actinobacteriota, we decided to evaluate the presence of bacteria that produce antibiotic molecules. Members of the phylum Actinobacteriota are known for their ability to produce antibiotic molecules, and many of them are found in association with higher organisms establishing symbiotic relationships (Matarrita-Carranza et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Antibiotic-producing Micrococcales govern the microbiome that inhabits the fur of two- and three-toed sloths

Rojas‐Gätjens¹,

Valverde-Madrigal²,

Rojas-Jiménez

et al. 2022

Preprint

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Sloths have a dense coat on which insects, algae, and fungi coexist in a symbiotic relationship. This complex ecosystem requires different levels of control, however, most of these mechanisms remain unknown. We investigated the bacterial communities inhabiting the hair of two- (Choloepus Hoffmani) and three-toed (Bradypus variegatus) sloths and evaluated their potential for producing antibiotic molecules capable of exerting control over the hair microbiota. The analysis of 16S rRNA amplicon sequence variants (ASVs) revealed that the communities in both host species are dominated by Actinobacteriota and Firmicutes. The most abundant genera were Brevibacterium, Kocuria/Rothia, Staphylococcus, Rubrobacter, Nesterenkonia, and Janibacter. In addition, we isolated nine strains of Brevibacterium and Rothia able to produce substances that inhibited the growth of common mammalian pathogens. The analysis of the biosynthetic gene clusters (BCGs) of these nine isolates suggests that the pathogen-inhibitory activity could be mediated by the presence of siderophores, terpenes, beta-lactones, Type III polyketide synthases (T3PKS), ribosomally synthesized, and post-translationally modified peptides (RiPPs), non-alpha poly-amino acids (NAPAA) like e-Polylysin, ectoine or nonribosomal peptides (NRPs). Our data suggest that Micrococcales inhabiting sloth hair could have a role in controlling microbial populations in that habitat, improving our understanding of this highly complex ecosystem.

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“…A novel compound cyphomycin was isolated from Streptomyces (ISID311) isolated from the microbiome of the fungus-growing ant which active against multidrug resistant fungal pathogens ( Chevrette et al, 2019 ). Matarrita-Carranza et al (2021) reported the genome sequence and the potential for antibiotic production of Streptomyces sp. M54, actinomycetes associated with the eusocial wasp ( Polybia plebeja ).…”

Section: Discussionmentioning

confidence: 99%

“…Actinomycetes are widely distributed in nature both in terrestrial and aquatic environments ( Goodfellow & Williams, 1983 ). They are recently found to be associated with several insects such as ants ( Currie et al, 1999 ; Currie et al, 2003 ; Cafaro & Currie, 2005 ; Oh et al, 2009 ; Van Arnam et al., 2016 ; Chevrette et al, 2019 ), leafcutter bees ( Inglis, Sinler & Goette, 1993 ), wasp ( Kroiss et al, 2010 ; Matarrita-Carranza et al, 2021 ), southern pine beetle ( Scott et al, 2008 ), honey bees and stingless bees ( Promnuan, Kudo & Chantawannakul, 2009 ; Promnuan, Kudo & Chantawannakul, 2011 ; Promnuan, Promsai & Meelai, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Apis andreniformis associated Actinomycetes show antimicrobial activity against black rot pathogen (Xanthomonas campestris pv. campestris)

Promnuan

Promsai

Pathom-aree

et al. 2021

PeerJ

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This study aimed to investigate cultivable actinomycetes associated with rare honey bee species in Thailand and their antagonistic activity against plant pathogenic bacteria. Actinomycetes were selectively isolated from the black dwarf honey bee (Apis andreniformis). A total of 64 actinomycete isolates were obtained with Streptomyces as the predominant genus (84.4%) followed by Micromonospora (7.8%), Nonomuraea (4.7%) and Actinomadura (3.1%). All isolates were screened for antimicrobial activity against Xanthomonas campestris pv. campestris, Pectobacterium carotovorum and Pseudomonas syringae pv. sesame. Three isolates inhibited the growth of X. campestris pv. campestris during in vitro screening. The crude extracts of two isolates (ASC3-2 and ASC5-7P) had a minimum inhibitory concentration (MIC) of 128 mg L−1against X. campestris pv. campestris. For isolate ACZ2-27, its crude extract showed stronger inhibitory effect with a lower MIC value of 64 mg L−1 against X. campestris pv. campestris. These three active isolates were identified as members of the genus Streptomyces based on their 16S rRNA gene sequences. Phylogenetic analysis based on the maximum likelihood algorithm showed that isolate ACZ2-27, ASC3-2 and ASC5-7P were closely related to Streptomyces misionensis NBRC 13063T (99.71%), Streptomyces cacaoi subsp. cacaoi NBRC 12748T (100%) and Streptomyces puniceus NBRC 12811T (100%), respectively. In addition, representative isolates from non-Streptomyces groups were identified by 16S rRNA gene sequence analysis. High similarities were found with members of the genera Actinomadura, Micromonospora and Nonomuraea. Our study provides evidence of actinomycetes associated with the black dwarf honey bee including members of rare genera. Antimicrobial potential of these insect associated Streptomyces was also demonstrated especially the antibacterial activity against phytopathogenic bacteria.

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Streptomyces sp. M54: an actinobacteria associated with a neotropical social wasp with high potential for antibiotic production

Cited by 12 publications

References 86 publications

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

Antibiotic-producing Micrococcales govern the microbiome that inhabits the fur of two- and three-toed sloths

Apis andreniformis associated Actinomycetes show antimicrobial activity against black rot pathogen (Xanthomonas campestris pv. campestris)

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