Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events

Rizzi, Aurora; Pontiroli, Alessandra; Brusetti, Lorenzo; Borin, Sara; Sorlini, C.; Abruzzese, A.; Sacchi, Gian Attilio; Vogel, Timothy M.; Simonet, Pascal; Bazzicalupo, Marco; Nielsen, Kaare M.; Monier, Jean-Michel; Daffonchio, Daniele

doi:10.1128/aem.02185-07

Cited by 40 publications

(46 citation statements)

References 26 publications

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“…More recently, other markers like the green fluorescent protein have been shown to allow monitoring of individual occurrences of HGT and provide accurate measures of their frequency (Babić et al, 2008;Perumbakkam et al, 2006;Sørensen et al, 2005). In some cases, the transformation frequencies determined from these studies are much greater than cultivation-based selection systems (Rizzi et al, 2008).…”

Section: Experimental Evidencementioning

confidence: 99%

Risks from GMOs due to Horizontal Gene Transfer

Keese¹

2008

Environ. Biosafety Res.

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Horizontal gene transfer (HGT) is the stable transfer of genetic material from one organism to another without reproduction or human intervention. Transfer occurs by the passage of donor genetic material across cellular boundaries, followed by heritable incorporation to the genome of the recipient organism. In addition to conjugation, transformation and transduction, other diverse mechanisms of DNA and RNA uptake occur in nature. The genome of almost every organism reveals the footprint of many ancient HGT events. Most commonly, HGT involves the transmission of genes on viruses or mobile genetic elements. HGT first became an issue of public concern in the 1970s through the natural spread of antibiotic resistance genes amongst pathogenic bacteria, and more recently with commercial production of genetically modified (GM) crops. However, the frequency of HGT from plants to other eukaryotes or prokaryotes is extremely low. The frequency of HGT to viruses is potentially greater, but is restricted by stringent selection pressures. In most cases the occurrence of HGT from GM crops to other organisms is expected to be lower than background rates. Therefore, HGT from GM plants poses negligible risks to human health or the environment.

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Section: Experimental Evidencementioning

confidence: 99%

Risks from GMOs due to Horizontal Gene Transfer

Keese¹

2008

Environ. Biosafety Res.

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“…5) Natural transformation of an engineered A. baylyi BD413 by externally-added DNA was also demonstrated using defrosted and slightly abraded tobacco leaves as a model system for a naturally decaying plant matter (Rizzi et al, 2008).…”

Section: )mentioning

confidence: 99%

“…However, transformation could only be detected if i) the transgenic plant was co-infected with a bacterial plant pathogen, and ii) if the plant transgene had homologous sequences with Acinetobacter, allowing homologous recombination. In absence of these criteria, the probability of natural transformation was very low and fell below the detection limit (Kay et al, 2002).3) Horizontal transfer of a plasmid from a tobacco plant to Acinetobacter (de Vries et al, 2004).4) Spread of recombinant DNA by roots and pollen of transgenic potato to Acinetobacter (de Vries et al, 2003).5) Natural transformation of an engineered A. baylyi BD413 by externally-added DNA was also demonstrated using defrosted and slightly abraded tobacco leaves as a model system for a naturally decaying plant matter (Rizzi et al, 2008).Horizontal genetic transfer to Acinetobacter via transformation was studied in animalia using A. baylyi BD413 and DNA harbouring the kanamycin resistance gene could not be detected in 1) the gut of grass grub larvae (Costelytra zealandica (White); Coleoptera: Scarabaeidae), at least not above the detection limit of 1 transformant per 10 3 cells, possibly due to low population density and limited growth of A. baylyi cells in grass grub guts (Ray et al, 2007), 2) in the gastrointestinal tract (GIT) of mice and rats, even under slightly positive selective pressure, at least not above the detection limit of 1 transformant per 10 3 -10 5 bacteria, possibly because exogenous DNA was readily degraded and absorbed in the GIT and the GIT environments was harsh and not conducive to survival of A. baylyi BD413 (Nordgård et al, 2007), nor 3) the gut of tobacco hornworm Manduca sexta (Lepidoptera) fed transgenic tobacco, even though BD413 survived transfer throught the gut (Deni et al 2005;Brinkmann and Tebbe, 2007). However, fecal matter containing transgenic DNA and DNA extracted from the fecal matter could transform A. baylyi BD413 (Brinkmann and Tebbe, 2007).…”

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confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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“…In one system, two counterselectable markers are used (13), while another involves green fluorescent protein (GFP). Transformation with a plant transgene can restore a promoter for gfp expression (14), or the transgene can restore fluorescence by repairing an inactive gfp allele (15). Fluorescence allows direct in situ visualization of HGT and has indicated the presence of transformation ''hotspots'' in plant tissue (14,16).…”

Section: Monitoring Horizontal Gene Transfermentioning

confidence: 99%

“…Transformation with a plant transgene can restore a promoter for gfp expression (14), or the transgene can restore fluorescence by repairing an inactive gfp allele (15). Fluorescence allows direct in situ visualization of HGT and has indicated the presence of transformation ''hotspots'' in plant tissue (14,16). During coinfection of tomato plants with the pathogen Ralstonia solanacearum, A. baylyi can be transformed with either R. solanacearum or plant transgenic sequences (17,18).…”

Section: Monitoring Horizontal Gene Transfermentioning

confidence: 99%

Acinetobacter baylyi ADP1: Transforming the choice of model organism

Elliott

Neidle

2011

IUBMB Life

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SummaryFor more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology studies that address a broad range of questions. Recently, the rapid accumulation of data from DNA sequencing, gene expression, protein structure, and other highthroughput methodology has increased the ability to tackle complex topics using sophisticated approaches to metabolic and genetic engineering. While the genetic malleability of ADP1 makes it an ideal organism for such investigations, A. baylyi ADP1 has yet to become a common choice for bacterial studies. This review describes examples of ADP1-based studies that exploit its highly efficient system for natural transformation and chromosomal incorporation of exogenous DNA. These studies focus on a wide array of problems, including gene duplication and amplification, horizontal gene transfer, bioreporters, and metabolic reconstruction. Interesting results in these diverse areas highlight the utility of using A. baylyi in laboratory and industrial settings.

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Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events

Cited by 40 publications

References 26 publications

Risks from GMOs due to Horizontal Gene Transfer

Risks from GMOs due to Horizontal Gene Transfer

Safety Assessment of Transgenic Organisms, Volume 4

Acinetobacter baylyi ADP1: Transforming the choice of model organism

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