Strategies for cell membrane functionalization

Armstrong, James R.; Perriman, Adam W.

doi:10.1177/1535370216650291

Cited by 19 publications

(20 citation statements)

References 112 publications

(146 reference statements)

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“…1–6 It provides a powerful means to elucidate molecular mechanisms by which specific biomolecules perform their functions and may find application in cell therapy by targeting therapeutic cells to specific tissues, 2,7–9 endowing cells with new functions such as recruiting/restoring protein binding or directing cell signaling or stem cell differentiation, 10–13 and altering immune cell responses toward cancerous cells. 14,15 …”

Section: Introductionmentioning

confidence: 99%

Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative

Capicciotti¹,

Zong²,

Sheikh³

et al. 2017

J. Am. Chem. Soc.

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Cell-surface engineering strategies that permit long-lived display of well-defined, functionally active molecules are highly attractive for eliciting desired cellular responses and for understanding biological processes. Current methodologies for the exogenous introduction of synthetic biomolecules often result in short-lived presentations, or require genetic manipulation to facilitate membrane attachment. Herein, we report a cell-surface engineering strategy that is based on the use of a CMP-Neu5Ac derivative that is modified at C-5 by a bifunctional entity composed of a complex synthetic heparan sulfate (HS) oligosaccharide and biotin. It is shown that recombinant ST6GAL1 can readily transfer the modified sialic acid to N-glycans of glycoprotein acceptors of living cells resulting in long-lived display. The HS oligosaccharide is functionally active, can restore protein binding, and allows activation of cell signaling events of HS-deficient cells. The cell-surface engineering methodology can easily be adapted to any cell type and is highly amenable to a wide range of complex biomolecules.

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Section: Introductionmentioning

confidence: 99%

Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative

Capicciotti¹,

Zong²,

Sheikh³

et al. 2017

J. Am. Chem. Soc.

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“…For metabolic-mediated modifications, a chemically reactive moiety is first incorporated into target biomolecules that are known as the "chemical reporters" by the endogenous machinery of the cell. In the second step, the reporter group is covalently reacted with a target functional biomolecules through bio-orthogonal ligation (or click chemistry) [33,36]. The most common chemical reporters, including azides and ketones, are used to metabolically incorporate to the cell surface by unnatural sialic acid biosynthesis.…”

Section: Covalent Chemical Conjugationsmentioning

confidence: 99%

“…Non-genetic cell surface engineering can be broadly divided into covalent chemical conjugation and noncovalent physical bioconjugation. Those different non-genetic cell surface engineering strategies are summarized in many recent reviews [10,19,25,26,[31][32][33][34][35]. Among these, the non-genetic cell surface engineering that is used to modulate and investigate cell functions and control cell-cell and cell-microenvironment interactions can be found in previous review articles [29,32,34].…”

Section: Introductionmentioning

confidence: 99%

Advances on Non-Genetic Cell Membrane Engineering for Biomedical Applications

Liu

2019

Polymers

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Cell-based therapeutics are very promising modalities to address many unmet medical needs, including genetic engineering, drug delivery, and regenerative medicine as well as bioimaging. To enhance the function and improve the efficacy of cell-based therapeutics, a variety of cell surface engineering strategies (genetic engineering and non-genetic engineering) are developed to modify the surface of cells or cell-based therapeutics with some therapeutic molecules, artificial receptors, and multifunctional nanomaterials. In comparison to complicated procedures and potential toxicities associated with genetic engineering, non-genetic engineering strategies have emerged as a powerful and compatible complement to traditional genetic engineering strategies for enhancing the function of cells or cell-based therapeutics. In this review, we will first briefly summarize key non-genetic methodologies including covalent chemical conjugation (surface reactive groups–direct conjugation, and enzymatically mediated and metabolically mediated indirect conjugation) and noncovalent physical bioconjugation (biotinylation, electrostatic interaction, and lipid membrane fusion as well as hydrophobic insertion), which have been developed to engineer the surface of cell-based therapeutics with various materials. Next, we will comprehensively highlight the latest advances in non-genetic cell membrane engineering surrounding different cells or cell-based therapeutics, including whole-cell-based therapeutics, cell membrane-derived therapeutics, and extracellular vesicles. Advances will be focused specifically on cells that are the most popular types in this field, including erythrocytes, platelets, cancer cells, leukocytes, stem cells, and bacteria. Finally, we will end with the challenges, future trends, and our perspectives of this relatively new and fast-developing research field.

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“…Protein changes on their surface ensure affinity to specific cell types, thus enhancing efficient and selected target cell communication ( 152 ). Cell modification is generally achieved either by hijacking biosynthesis to favor the production of specific endogenous material or by delivering exogenous species to the cytoplasmic membrane ( 158 ). Main current small EVs engineering strategies are summarized in Table 3 .…”

Section: Small Evs Treatment In the Infarcted Myocardium: Novel Theramentioning

confidence: 99%

Extracellular Vesicle-Mediated Immune Regulation of Tissue Remodeling and Angiogenesis After Myocardial Infarction

Sánchez‐Alonso¹,

Alcaraz‐Serna²,

Sánchez-Madrid³

et al. 2018

Front. Immunol.

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Myocardial ischemia-related disorders constitute a major health problem, being a leading cause of death in the world. Upon ischemia, tissue remodeling processes come into play, comprising a series of inter-dependent stages, including inflammation, cell proliferation and repair. Neovessel formation during late phases of remodeling provides oxygen supply, together with cellular and soluble components necessary for an efficient myocardial reconstruction. Immune system plays a central role in processes aimed at repairing ischemic myocardium, mainly in inflammatory and angiogenesis phases. In addition to cellular components and soluble mediators as chemokines and cytokines, the immune system acts in a paracrine fashion through small extracellular vesicles (EVs) release. These vesicular structures participate in multiple biological processes, and transmit information through bioactive cargoes from one cell to another. Cell therapy has been employed in an attempt to improve the outcome of these patients, through the promotion of tissue regeneration and angiogenesis. However, clinical trials have shown variable results, which put into question the actual applicability of cell-based therapies. Paracrine factors secreted by engrafted cells partially mediate tissue repair, and this knowledge has led to the hypothesis that small EVs may become a useful tool for cell-free myocardial infarction therapy. Current small EVs engineering strategies allow delivery of specific content to selected cell types, thus revealing the singular properties of these vesicles for myocardial ischemia treatment.

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Strategies for cell membrane functionalization

Cited by 19 publications

References 112 publications

Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative

Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative

Advances on Non-Genetic Cell Membrane Engineering for Biomedical Applications

Extracellular Vesicle-Mediated Immune Regulation of Tissue Remodeling and Angiogenesis After Myocardial Infarction

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